Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,736	A→G	M72T (ATG→ACG)	ybiA ←	N‑glycosidase YbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,736	0	A	G	100.0%	138.8 / NA	39	M72T (ATG→ACG)	ybiA	N‑glycosidase YbiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/13);  total (26/13)

TGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAA  >  NC_000913/832600‑832865                                                                                                                                         |                                                                                                                                  tGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAg                                                                                                                                <  1:537159/140‑1 (MQ=255)  gCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAgg                                                                                                                               <  2:629155/140‑1 (MQ=255)          gagCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGaaa                                                                                                                       >  1:88135/1‑140 (MQ=255)              gcacgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCg                                                                                                                   <  1:122292/140‑1 (MQ=255)                acgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGAc                                                                                                                 >  1:637874/1‑140 (MQ=255)                  gcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACgg                                                                                                               >  1:461303/1‑140 (MQ=255)                  gcaGTTCTGCATGCTATTCGAAGTTAGCGTGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACgg                                                                                                               <  1:243732/140‑1 (MQ=255)                        cTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAA‑GATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCtt                                                                                                        >  1:76719/1‑140 (MQ=255)                                tGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTa                                                                                                 >  1:364995/1‑140 (MQ=255)                                tGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTa                                                                                                 >  2:14670/1‑140 (MQ=255)                                tGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTa                                                                                                 >  2:123285/1‑140 (MQ=255)                                        tttAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGTGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcat                                                                                         >  1:248006/1‑140 (MQ=255)                                        tttAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcat                                                                                         <  2:637874/140‑1 (MQ=255)                                          tAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcatca                                                                                       >  2:101724/1‑140 (MQ=255)                                            gcgcGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAg                                                                                     >  2:414439/1‑140 (MQ=255)                                            gcgcGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAg                                                                                     >  2:16485/1‑140 (MQ=255)                                             cgcgAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTcgcg                                                                                                    <  2:543684/124‑1 (MQ=255)                                             cgcgAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTcgcg                                                                                                    >  1:543684/1‑124 (MQ=255)                                                       ttttCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTtgtg                                                                          <  1:14670/140‑1 (MQ=255)                                                              aTCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGaaa                                                                   >  1:598454/1‑140 (MQ=255)                                                                 aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATa                                                                <  1:123285/140‑1 (MQ=255)                                                                 aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATa                                                                <  1:417878/140‑1 (MQ=255)                                                                      ttCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAGTTTTCATAAAGGAAGTTTTGTACCTGAAAATAGTGtt                                                           >  1:217384/1‑140 (MQ=255)                                                                       tCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTc                                                          <  2:517509/140‑1 (MQ=255)                                                                          ttGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGa                                                       >  1:138092/1‑140 (MQ=255)                                                                             aCCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGt                                                    >  1:60106/1‑140 (MQ=255)                                                                                gACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAgg                                                 >  2:530480/1‑140 (MQ=255)                                                                                gACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAgg                                                 >  2:57980/1‑140 (MQ=255)                                                                                            ttACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTccc                                     >  1:350610/1‑140 (MQ=255)                                                                                                   gagGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAcc                              >  1:33786/1‑140 (MQ=255)                                                                                                       cTTAGAACGATCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGa                          <  2:598454/140‑1 (MQ=255)                                                                                                              cGATCGCGCCCCATGCGAGCGGGGACCGTAGAGGAAGAACCCCGACGGTTCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCa                   <  2:248006/140‑1 (MQ=255)                                                                                                                aTCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                 >  1:468004/1‑140 (MQ=255)                                                                                                                aTCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                 >  2:546564/1‑140 (MQ=255)                                                                                                                aTCGCGCCCCATGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTATTCCCGTCAACCTTGATGGGCCAgg                 >  1:2818/1‑140 (MQ=255)                                                                                                                          aTGCGTGCGGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGtt       <  1:414439/140‑1 (MQ=255)                                                                                                                               tgcgGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaa  >  2:449486/1‑140 (MQ=255)                                                                                                                               tgcgGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaa  >  2:158893/1‑140 (MQ=255)                                                                                                                               tgcgGCGACCGTAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaa  >  2:9038/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  TGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAA  >  NC_000913/832600‑832865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑