Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	843,979	A→G	L501P (CTA→CCA)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	843,979	0	A	G	100.0%	71.9 / NA	22	L501P (CTA→CCA)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

ATGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCCGCGCC  >  NC_000913/843842‑844109                                                                                                                                          |                                                                                                                                   aTGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgc                                                                                                                                  >  1:573924/1‑140 (MQ=255)    ccccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcggg                                                                                                                               <  1:526740/140‑1 (MQ=255)    ccccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcggg                                                                                                                               <  2:509725/140‑1 (MQ=255)     cccGCCACCTGCCAGCACTGGGTCGATATTGTCGCCGATATCCGACAAAACATTCATGATGGTGATGGTACTGATAATCCCCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcgggc                                                                                                                              <  2:268729/140‑1 (MQ=255)      ccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTggcgggcg                                                                                                                             >  2:105238/1‑140 (MQ=255)         gCACCTGCCAGCAATCGCGCGACATCGACTCCGATTTTCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGcc                                                                                                                          <  1:172136/140‑1 (MQ=255)                       tGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAag                                                                                                            >  2:619885/1‑140 (MQ=255)                                      gCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGa                                                                                             <  1:103272/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTCCTGCTAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                        <  2:234214/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGTCGCTCGGTGGCGGCCGGACATGAATATCCGAAGCCACCCGGTTTTCGATCaaa                                                                                        >  2:288267/1‑140 (MQ=255)                                                   aacaCAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAg                                                                                <  2:30031/140‑1 (MQ=255)                                                     cacaATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCa                                                                              >  1:403168/1‑140 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGcc                                                                    >  1:643333/1‑140 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGcc                                                                    >  1:3461/1‑140 (MQ=255)                                                                     atggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAAccgc                                                              >  1:376111/1‑140 (MQ=255)                                                                                    cccGCCAGCGGGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAAt                                               <  1:638465/139‑1 (MQ=255)                                                                                    cccGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAAt                                               <  1:518467/139‑1 (MQ=255)                                                                                             gcgTTACGAAACAGCCTAAGCACGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCTGAGAAGAAAAGAATGAGTGCGAt                                      <  1:219489/140‑1 (MQ=255)                                                                                              cgTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATa                                     <  2:403168/140‑1 (MQ=255)                                                                                                    gAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATc                               <  1:5315/140‑1 (MQ=255)                                                                                                      aaCAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAg                             <  1:128971/140‑1 (MQ=255)                                                                                                           cGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGatta                        >  2:521197/1‑138 (MQ=255)                                                                                                                        cgcgTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGGGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTg           >  2:177048/1‑140 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGTTACGGATCAGGATATCTACGGTTTTCTGCCCcgc     >  2:341036/1‑140 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcgc     >  1:568529/1‑140 (MQ=255)                                                                                                                               gggCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcgcg    <  1:426794/140‑1 (MQ=255)                                                                                                                                 gcgcTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCCGCGcc  <  2:643333/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   ATGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCCGCGCC  >  NC_000913/843842‑844109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑