Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	859,010	T→C	I18V (ATC→GTC)	ybiU ←	DUF1479 domain‑containing protein YbiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	859,010	0	T	C	100.0%	122.7 / NA	37	I18V (ATC→GTC)	ybiU	DUF1479 domain‑containing protein YbiU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

CACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGC  >  NC_000913/858873‑859137                                                                                                                                          |                                                                                                                                 cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCGAGCTGCGCCCGTAGCGCGCGCATCATCTGACGGACAg                                                                                                                                >  1:279468/1‑140 (MQ=255) cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAg                                                                                                                                >  2:400047/1‑140 (MQ=255) cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAg                                                                                                                                >  1:45669/1‑140 (MQ=255) cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGACCGCAGCGCGTGCTTCATCTGACGGACAg                                                                                                                                >  1:298100/1‑140 (MQ=255) cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGCCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAg                                                                                                                                >  1:631289/1‑140 (MQ=255) cACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCAGGACGTCGACAAGCTGGGCCCGCAGAGCGTCCTTCATCTGACGGACAg                                                                                                                                >  2:186830/1‑140 (MQ=255)   ccGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAgct                                                                                                                              <  1:400047/140‑1 (MQ=255)      gCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAgctgct                                                                                                                           >  1:144926/1‑140 (MQ=255)              gCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGAGCGCGCTTCATCCGACGCACAGCTGCTTTGTGATc                                                                                                                   >  2:267777/1‑140 (MQ=255)                         tgCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAAtg                                                                                                        >  1:40599/1‑140 (MQ=255)                         tgCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCCTCATCTGACGGACAGCAGCTTCGTGATCGGCAGGCAAtg                                                                                                        >  2:278115/1‑140 (MQ=255)                                aGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCt                                                                                                 >  1:106139/1‑140 (MQ=255)                                   gCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGt                                                                                              >  2:517878/1‑140 (MQ=255)                                           ttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAg                                                                                      <  1:528790/140‑1 (MQ=255)                                           ttCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAg                                                                                      <  2:397386/140‑1 (MQ=255)                                              aGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGGAGGGAATGTGTCGATGGTAAAAGTAGAAg                                                                                   >  1:213590/1‑140 (MQ=255)                                              aGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAg                                                                                   <  1:141965/140‑1 (MQ=255)                                                ccACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGcc                                                                                 >  2:667143/1‑140 (MQ=255)                                                   cTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATa                                                                              >  1:524515/1‑140 (MQ=255)                                                     cgcgTGGCAATGTCATCGCTTACCTGATTAATGATCTGCGGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAac                                                                            <  2:231049/140‑1 (MQ=255)                                                                     cGCTTAGCTGATTAACGATCTGTTGCACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑Ctt                                                           <  1:186830/140‑1 (MQ=255)                                                                          aGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCAtt                                                      <  2:471498/140‑1 (MQ=255)                                                                           gCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTc                                                     >  1:117760/1‑140 (MQ=255)                                                                                         tgctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGAt                                       >  1:546154/1‑140 (MQ=255)                                                                                         tgctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCGCATAAA‑TTTTCATCCGAAACCATTCTGAt                                       >  1:206623/1‑140 (MQ=255)                                                                                         tgctgGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGAAGAAGCCACAACCACCACTCATTAA‑CTTTCTTTCGAAACCAATCTGAt                                       >  2:238297/1‑140 (MQ=255)                                                                                                   cGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGa                             >  2:370046/1‑140 (MQ=255)                                                                                                         gCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAatat                       <  1:667143/140‑1 (MQ=255)                                                                                                         gCTGCGCCCGCAGCGCGTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAatat                       <  2:39157/140‑1 (MQ=255)                                                                                                              gcCCGCAGCGCGTGCTTCATCTTACGGACAGGTGCCTTGTGATCGGCATGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGtt                  <  2:213590/140‑1 (MQ=255)                                                                                                              gcCCGCAGCGCGTGCATCATCTAACCGACCGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGtt                  <  1:199356/140‑1 (MQ=255)                                                                                                               cccGCAGCTCGTGCTTCCTCTGTCGGACAGCCGCTGTGTGATCGGCAGACAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAACCTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGtt                  <  2:298100/140‑1 (MQ=255)                                                                                                                       gcgTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAg         <  1:566483/140‑1 (MQ=255)                                                                                                                        cgTGCTTCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGt        <  1:517878/140‑1 (MQ=255)                                                                                                                             ttCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATg   <  2:144926/140‑1 (MQ=255)                                                                                                                              tCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGc  >  1:548268/1‑140 (MQ=255)                                                                                                                              tCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGc  <  2:45669/140‑1 (MQ=255)                                                                                                                              tCATCTGACGGACAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGc  <  1:33577/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CACCGGCCAGACGGCATCGCCCTGTGCTTTGAGTGCGTTGATTTCAGCCACTCGCGTGGCAATGTCATCGCTTAGCTGATTAAAGATCTGCTGGACGTCGCCAAGCTGCGCCCGCAGCGCGTGCTTCATCTGACGGATAGCTGCTTTGTGATCGGCAGGCAATGTGTCGCTGGTAAAAGTAGAAGCCATAACCACCTCTCATTAA‑CTTTCATTCGAAACCATTCTGATTTGCATGTGACAATATAAGTTATAAATAAGTTAATGC  >  NC_000913/858873‑859137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑