Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	62,678	A→G	L196P (CTC→CCC)	rapA ←	RNA polymerase‑binding ATPase and RNAP recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	62,678	0	A	G	100.0%	133.4 / NA	38	L196P (CTC→CCC)	rapA	RNA polymerase‑binding ATPase and RNAP recycling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/13);  total (25/13)

TGCTGAGCTTCGGCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTAC  >  NC_000913/62546‑62813                                                                                                                                     |                                                                                                                                        tGCTGAGCTTCGGCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTg                                                                                                                                  <  1:440619/140‑1 (MQ=255)      aGCTTCGGCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGAt                                                                                                                             >  1:124896/1‑140 (MQ=255)           cGGCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAgg                                                                                                                        >  2:76189/1‑140 (MQ=255)             gCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGat                                                                                                                      >  1:587218/1‑140 (MQ=255)                                 tAGCGCAAAGCGCAGGTCGAAACGGCGCAGCTTTTATACTAGCCACTGTTGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGttt                                                                                                  <  2:197140/140‑1 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGGCAGAGGGCAGTTTCTGTTGCAGGATAATCCTGGCTTCAATGGTTTTcc                                                                                               >  2:171633/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGGCAGAGGGCAGTTGCTGGTGCAGGATAATCCTGGCTTCAATGGTTTTcc                                                                                               >  2:206000/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGTATTATCCCGGCTTCAATGGTTTTcc                                                                                               >  1:197140/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTcc                                                                                               >  1:427328/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTcc                                                                                               >  1:517183/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTcc                                                                                               >  2:144850/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTcc                                                                                               >  2:4201/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTcc                                                                                               >  2:104046/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGATGCTGATGCAGAATAATCCCGGCTTCAATGGTTTTcc                                                                                               >  1:328376/1‑140 (MQ=255)                                    cgcAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGATGCTGATACAGGATGATCCCGTCTTCAATGGTTTTcc                                                                                               >  1:297912/1‑140 (MQ=255)                                      cAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCt                                                                                             >  1:465965/1‑140 (MQ=255)                                             cAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACTCa                                                                                      <  2:427328/140‑1 (MQ=255)                                                   gAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGt                                                                                <  2:517183/140‑1 (MQ=255)                                                    aaaCGGCGTAGCATTTCCACCAGCCACTGAGTCCGTAAGATTTCCGGGGCGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTc                                                                               <  2:328376/140‑1 (MQ=255)                                                          cgcAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTcagccag                                                                         >  1:565868/1‑140 (MQ=255)                                                                          gCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACgcgcggcgcg                                                         <  2:417297/140‑1 (MQ=255)                                                                               tGATGCTGTAAGGTTTCTGGGAGGATAATTAGCACGCGTGCAGCAGCGGCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTggcg                                                    <  2:237624/140‑1 (MQ=255)                                                                               tGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTggcg                                                    <  2:565868/140‑1 (MQ=255)                                                                                 aTGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTggcggc                                                  <  2:587218/140‑1 (MQ=255)                                                                                     tGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATTCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGAcc                                              >  1:494150/1‑140 (MQ=255)                                                                                         aGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTTAGCCAGCAGGAAGCGCGGCGCATGGCGGCGACCAAca                                          >  1:264586/1‑140 (MQ=255)                                                                                              tCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATg                                     >  2:412134/1‑140 (MQ=255)                                                                                               ccGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGa                                    >  1:97429/1‑140 (MQ=255)                                                                                                    aCGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCTAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGAt                               >  1:130392/1‑140 (MQ=255)                                                                                                     cGATAATTAGCACACGTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATg                              >  1:542302/1‑140 (MQ=255)                                                                                                                    gTTCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCggg               >  2:167517/1‑140 (MQ=255)                                                                                                                     ttCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCCAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGa              <  2:386505/140‑1 (MQ=255)                                                                                                                      tCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGAt             <  2:124896/140‑1 (MQ=255)                                                                                                                      tCAGCAGCGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGAt             <  1:638508/140‑1 (MQ=255)                                                                                                                        agcagcGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCa           <  1:76189/140‑1 (MQ=255)                                                                                                                        agcagcGCCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCa           >  2:637082/1‑140 (MQ=255)                                                                                                                              gcCAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTgg     >  2:113262/1‑140 (MQ=255)                                                                                                                               ccAGAGGGCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGt    >  1:465527/1‑140 (MQ=255)                                                                                                                                 agagggCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTAc  <  2:464244/140‑1 (MQ=255)                                                                                                                                 agagggCGGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTAc  <  2:576939/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TGCTGAGCTTCGGCATAACGCTCATCATCAAATAGCGCAAAGCGCAGGTTGAAACGGCGCAGCATTTCTACCAGCCACTGATGCTGTAAGGTTTCCGGGACGATAATTAGCACACGTTCAGCAGCGCCAGAGAGCAGTTGCTGATGCAGGATCATCCCGGCTTCAATGGTTTTCCCTAAACCCACTTCGTCAGCCAGCAGGACGCGCGGCGCGTGGCGGCGACCAACATCATGAGCGATGTTGAGCTGATGCGGGATCAGGCTGGTAC  >  NC_000913/62546‑62813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑