Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 871,743 A→G G259G (GGA→GGG)  gsiC → glutathione ABC transporter membrane subunit GsiC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913871,7430AG97.4% 119.9 / NA 38G259G (GGA→GGG) gsiCglutathione ABC transporter membrane subunit GsiC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/16);  minor base T (1/0);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGACGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCGGCTATCAGGT  >  NC_000913/871608‑871879
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cGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCt                                                                                                                                      <  1:445220/140‑1 (MQ=255)
          gTTGTCCTCAAACACGGGCTACGTACCGCCATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGa                                                                                                                            <  1:245965/140‑1 (MQ=255)
            tGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACt                                                                                                                          <  2:423014/140‑1 (MQ=255)
             gTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTCTCAACTGTCCGTGAGTTGGGCACTTACTCGTTGACTc                                                                                                                         >  1:318314/1‑140 (MQ=255)
                     acacGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGaaa                                                                                                                 >  1:640235/1‑140 (MQ=255)
                          ggCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGt                                                                                                            <  1:162484/140‑1 (MQ=255)
                              aCGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGAGTTGGGCGCTTACTCGTAGACTCCGTAGAAATGCGTGAtt                                                                                                        >  2:229544/1‑140 (MQ=255)
                               cGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACATGGGCGCTTACTCGTTAACTCAGTAGAAATGCGTGATTa                                                                                                       >  2:204796/1‑140 (MQ=255)
                               cGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTAGCCGGGACTTGGTCGCATACTCGTTGACTCGGTAGAAATGCGTGATTa                                                                                                       >  2:276913/1‑140 (MQ=255)
                                       gatgatCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGa                                                                                               >  1:627845/1‑140 (MQ=255)
                                           atCCCGGTAGTGACCCTGATGGTCTTACACTTTGGCTCTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCa                                                                                           <  1:276913/140‑1 (MQ=255)
                                                     tGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAAtt                                                                                 >  1:65349/1‑140 (MQ=255)
                                                       aCCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCt                                                                               >  1:166697/1‑140 (MQ=255)
                                                       aCCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTACAAATGCGAGATGACGCGGTGAGTCAGGCGGAAATTCt                                                                               >  1:174155/1‑140 (MQ=255)
                                                       aCCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGCTGACTCCGCAGACACGAGTGATTACTCGGTGATTCAGGCGGAAATTCt                                                                               >  2:304743/1‑140 (MQ=255)
                                                            gatgGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAAtt                                                                                 >  2:156848/1‑133 (MQ=255)
                                                            gatgGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAAtt                                                                                 <  1:156848/133‑1 (MQ=255)
                                                                 gCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCg                                                                     <  2:166697/140‑1 (MQ=255)
                                                                  cTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGAGTACCCGGGGATTCATGCGGGAATTCTGCTTTTCTCGc                                                                    >  1:223287/1‑140 (MQ=255)
                                                                   ttACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTGTTCAACTGGCCGGGACTTTGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCt                                                                   >  1:157405/1‑140 (MQ=255)
                                                                    tACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTg                                                                  >  1:620374/1‑140 (MQ=255)
                                                                           tGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTa                                                           >  2:622360/1‑140 (MQ=255)
                                                                           tGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTa                                                           >  2:573530/1‑140 (MQ=255)
                                                                             gCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTAtt                                                         <  1:622360/140‑1 (MQ=255)
                                                                             gCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTAtt                                                         <  2:620374/140‑1 (MQ=255)
                                                                                  ttGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTAt                                                    <  1:573530/140‑1 (MQ=255)
                                                                                  ttGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTAt                                                    <  2:540279/140‑1 (MQ=255)
                                                                                   tGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGGGGAAATTATGCGTTACTCGCTGGAATTTATTCTTATc                                                   >  1:171560/1‑140 (MQ=255)
                                                                                           ggtTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAgt                                           <  2:627845/140‑1 (MQ=255)
                                                                                            gtTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTGACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAgtg                                          <  2:279587/140‑1 (MQ=255)
                                                                                                  tCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTCCTCGCTGGAATTTACTCTTATAAACTTAGTGGTGGAt                                    >  1:328527/1‑140 (MQ=255)
                                                                                                    gTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGtggtgg                                      >  2:573588/1‑136 (MQ=255)
                                                                                                    gTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGtggtgg                                      <  1:573588/136‑1 (MQ=255)
                                                                                                       gtgGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCt                               >  2:422131/1‑140 (MQ=255)
                                                                                                       gtgGAGAAAGTTTTCAACTGGCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGCGATTACCCGGTGATTCAGGCGGAAATTCAGCTTTTCTAGCTGGCAGTTATTCTTCTCAACTTAGTGGTGGATGTGCt                               >  2:316981/1‑140 (MQ=255)
                                                                                                                           gCCGGGACTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCgg           >  1:472472/1‑140 (MQ=255)
                                                                                                                                 aCTTGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCGGCTATCa     >  1:489146/1‑140 (MQ=255)
                                                                                                                                    tGGGCGCTTGCTCGTTGACTCCGTAGAAATGCGTGATTACCCGTTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCGGCTATCAGGt  <  2:5334/140‑1 (MQ=255)
                                                                                                                                    tGGGCGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCGGCTATCAGGt  <  1:422131/140‑1 (MQ=255)
                                                                                                                                    tGGGCGCTTACTCGTAGACTCCATACAAAAGCGTGATAACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTATCCCGGCTATCAGGt  <  1:316981/140‑1 (MQ=255)
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CGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGGCTTTTTGCTCGGTGGTTCCATCGTTGTGGAGAAAGTTTTCAACTGGCCGGGACTTGGACGCTTACTCGTTGACTCCGTAGAAATGCGTGATTACCCGGTGATTCAGGCGGAAATTCTGCTTTTCTCGCTGGAATTTATTCTTATCAACTTAGTGGTGGATGTGCTTTACGCCGCCATTAACCCGGCTATCAGGT  >  NC_000913/871608‑871879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑