Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	910,426	A→G	C208C (TGT→TGC)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,426	0	A	G	100.0%	69.8 / NA	21	C208C (TGT→TGC)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

TACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAC  >  NC_000913/910313‑910558                                                                                                                  |                                                                                                                                     tACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATa                                                                                                            >  2:482282/1‑140 (MQ=255)      aTTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGCAATAACgca                                                                                                       <  1:640003/140‑1 (MQ=255)        tATCGTATTCGACATTTTCTTTGCCCCGCGGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACgcagc                                                                                                     <  1:221512/140‑1 (MQ=255)         aTCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCGGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACgcagca                                                                                                    >  2:149214/1‑140 (MQ=255)                    cATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGt                                                                                         >  2:358014/1‑140 (MQ=255)                    cATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGt                                                                                         >  2:126144/1‑140 (MQ=255)                          cTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGt                                                                                   >  2:487307/1‑140 (MQ=255)                                 gcgcAGGGCACGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACtctt                                                                            >  2:131001/1‑140 (MQ=255)                                  cgcAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGGGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCACCAGTTTGCGTAACtcttc                                                                           >  2:662111/1‑140 (MQ=255)                                  cgcAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGGGATATTGCTGGAATAACGCAGCAGTGGCGCAAGTTTGCGTAACtcttc                                                                           >  2:170435/1‑140 (MQ=255)                                    cAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACtcttctt                                                                         >  2:349390/1‑140 (MQ=255)                                       ggCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTcttcttctt                                                                      >  1:151236/1‑140 (MQ=255)                                                             aTTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTgg                                                <  1:358014/140‑1 (MQ=255)                                                               tttCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGgcg                                              <  2:157594/140‑1 (MQ=255)                                                                  cccGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCAt                                           <  1:34849/140‑1 (MQ=255)                                                                       cAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATAc                                      <  2:161918/140‑1 (MQ=255)                                                                       cAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATAc                                      <  1:149214/140‑1 (MQ=255)                                                                       cAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTAACGACTGGTTGTGGCGCATGATAc                                      <  1:345815/140‑1 (MQ=255)                                                                                               cccccGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGgc              >  2:590312/1‑140 (MQ=255)                                                                                                  ccGCGCAGCCGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCa           <  1:62410/140‑1 (MQ=255)                                                                                                           cGCTGCCGCACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAc  <  1:131001/140‑1 (MQ=255)                                                                                                                  |                                                                                                                                     TACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGCCCCTTCTGGCGCAGGTTTTAAC  >  NC_000913/910313‑910558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑