Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	950,957	T→C	T42A (ACC→GCC)	pflA ←	pyruvate formate‑lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	950,957	0	T	C	100.0%	100.7 / NA	29	T42A (ACC→GCC)	pflA	pyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/15);  total (14/15)

ACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCAATAACTGACATTGC  >  NC_000913/950822‑951083                                                                                                                                        |                                                                                                                               aCTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTcg                                                                                                                            >  1:370781/1‑140 (MQ=255)                 tGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTc                                                                                                                             <  1:281312/123‑1 (MQ=255)                 tGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTc                                                                                                                             <  1:360682/123‑1 (MQ=255)                 tGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTc                                                                                                                             >  2:281312/1‑123 (MQ=255)                 tGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTc                                                                                                                             >  2:360682/1‑123 (MQ=255)                   cTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAgg                                                                                                          <  2:32072/140‑1 (MQ=255)                     tCACCGCCGGATGCGGTGACGCGGCCGCCGGCAGCTTTCCTAAAGTGGTGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCa                                                                                                        <  2:206434/140‑1 (MQ=255)                     tCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCa                                                                                                        <  2:130613/140‑1 (MQ=255)                                tGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGc                                                                                             >  2:610628/1‑140 (MQ=255)                                                         ttCATAAATTGGCGATGCGTCACCACTTCCATCATCAAATCTTCAACGTTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCg                                                                    <  2:321478/140‑1 (MQ=255)                                                                   ggCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACGGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTggg                                                          <  2:370781/140‑1 (MQ=255)                                                                    gCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGc                                                         >  2:440067/1‑140 (MQ=255)                                                                    gCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGc                                                         >  1:88559/1‑140 (MQ=255)                                                                    gCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCATGGAAAAACGTGACAAAGCGAATACCTGGGc                                                         >  2:286131/1‑140 (MQ=255)                                                                        tAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTc                                                     <  2:540838/140‑1 (MQ=255)                                                                                  cttccttcATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca                                           <  1:429057/140‑1 (MQ=255)                                                                                   ttccttcATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCcac                                          <  2:62844/140‑1 (MQ=255)                                                                                      cttcATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAgg                                       >  1:148770/1‑140 (MQ=255)                                                                                           tcaAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCa                                  <  1:74101/140‑1 (MQ=255)                                                                                            caAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGAATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCaa                                 <  1:413891/140‑1 (MQ=255)                                                                                               aTCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAgg                              >  2:634292/1‑140 (MQ=255)                                                                                                            aCTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGAcc                 <  1:62569/140‑1 (MQ=255)                                                                                                            aCTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGAcc                 >  2:452647/1‑140 (MQ=255)                                                                                                            aCTTCTTTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGAcc                 <  1:390935/140‑1 (MQ=255)                                                                                                             cttcttTACCGCCATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCa                <  1:452647/140‑1 (MQ=255)                                                                                                                    accaccATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCAATAACTg         >  1:100778/5‑140 (MQ=255)                                                                                                                        ccATGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCAGAGGATTCGAAGCAGTGAATGCAACCAATAACTGACAt     >  1:254287/1‑140 (MQ=255)                                                                                                                          aTGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGGGAATGCGACCAATAACTGACATTg   >  2:129525/1‑140 (MQ=255)                                                                                                                           tGCGTGTCCCAGGCGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGACTGCGACTAATAACTGACATTGc  >  1:203900/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               ACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGGAGTGAATGCGACCAATAACTGACATTGC  >  NC_000913/950822‑951083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑