Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	975,737	A→G	D39G (GAC→GGC)	mukE →	chromosome partitioning protein MukE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	975,737	0	A	G	100.0%	119.7 / NA	35	D39G (GAC→GGC)	mukE	chromosome partitioning protein MukE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

TACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCCCACGTTCCACCACGCTGATCCCT  >  NC_000913/975600‑975873                                                                                                                                          |                                                                                                                                         tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcggcg                                                                                                                                        >  2:449598/1‑140 (MQ=255) tACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcggcg                                                                                                                                        >  1:666191/1‑140 (MQ=255)   cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGaa                                                                                                                                      <  1:107732/140‑1 (MQ=255)   cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGaa                                                                                                                                      >  2:516463/1‑140 (MQ=255)      aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTg                                                                                                                                   >  1:97221/1‑140 (MQ=255)       gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTgg                                                                                                                                  <  1:22323/140‑1 (MQ=255)        ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGa                                                                                                                                 <  2:161383/140‑1 (MQ=255)        ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGa                                                                                                                                 >  1:161383/1‑140 (MQ=255)        ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGa                                                                                                                                 <  2:334301/140‑1 (MQ=255)        ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGa                                                                                                                                 <  2:334293/140‑1 (MQ=255)           aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATaa                                                                                                                              <  1:8142/140‑1 (MQ=255)                cAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATg                                                                                                                         <  1:434654/140‑1 (MQ=255)                       aTGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCt                                                                                                                  >  1:491376/1‑140 (MQ=255)                       aTGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCt                                                                                                                  >  1:648435/1‑140 (MQ=255)                        tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGATGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTg                                                                                                                 >  2:152670/1‑140 (MQ=255)                        tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTg                                                                                                                 >  2:57412/1‑140 (MQ=255)                                          aaCAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACAGGATAATCATGCATACCTGATCGATTTTCAGGAatat                                                                                               >  1:213282/1‑140 (MQ=255)                                           aCAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGATGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATc                                                                                              <  1:152670/140‑1 (MQ=255)                                               gTGATGCCGGTTAAGGTGGCGCAGGCGCTTGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                                                                                          <  1:242684/140‑1 (MQ=255)                                               gTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                                                                                          >  2:474733/1‑140 (MQ=255)                                                         ttAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAc                                                                                >  1:472327/1‑140 (MQ=255)                                                          tAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcg                                                                               <  1:632145/140‑1 (MQ=255)                                                            aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                                             <  1:449598/140‑1 (MQ=255)                                                            aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                                             <  2:491376/140‑1 (MQ=255)                                                                 gcgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTta                                                                        >  1:655588/1‑140 (MQ=255)                                                                                     gTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcgcg                                                    <  2:664369/140‑1 (MQ=255)                                                                                     gTTATTTCCGGCGCTAGACAGCGCCTTACGTTCAGGAGGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcgcg                                                    <  1:244079/140‑1 (MQ=255)                                                                                       tATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCa                                                  >  1:645366/1‑140 (MQ=255)                                                                                           tCCGGCGCTGGACAGCGCCTTATGTTCAGCACGCCATCTTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAg                                              <  1:218285/140‑1 (MQ=255)                                                                                                cgcTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg                                         <  1:57412/140‑1 (MQ=255)                                                                                                cgcTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg                                         <  1:474733/140‑1 (MQ=255)                                                                                                cgcTGGACAGCGCCTCACGTTCCGGGCGCTATATTGGTCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg                                         <  1:197858/140‑1 (MQ=255)                                                                                                            ccTTACGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTa                             <  2:645366/140‑1 (MQ=255)                                                                                                                aCGTTCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGcc                         <  2:666191/140‑1 (MQ=255)                                                                                                                    tCAGGACGCCATATTGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCCCACg                     >  2:561021/1‑140 (MQ=255)                                                                                                                                  tGGCCTCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCCCACGTTCCACCACGCTGa       >  2:373671/1‑140 (MQ=255)                                                                                                                                       tCGGCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAGGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCCCACGTTCCACCACGCTGATcccc  >  1:664169/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                         TACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCCCACGTTCCACCACGCTGATCCCT  >  NC_000913/975600‑975873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑