| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 977,713 | A→G | Q463R (CAG→CGG) | mukB → | chromosome partitioning protein MukB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 977,713 | 0 | A | G | 96.8% | 103.1 / NA | 31 | Q463R (CAG→CGG) | mukB | chromosome partitioning protein MukB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (14/16); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTA > NC_000913/977576‑977828 | ccAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAATAGCAGGAAGCGACTGAAAAAAGGCTCTCTCTTGAGCGGa > 2:235952/1‑140 (MQ=255)ccAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAGGCGACTGAAAAAAAGCTCTCTCTTGAGCGGa > 2:206508/1‑140 (MQ=255)ccAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCAGGAAGCGACTGAAAAAAAGCTCTCTCTTGAGCGGa > 2:282337/1‑140 (MQ=255) aGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGaaa > 1:638638/1‑140 (MQ=255) gCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAAt > 1:137864/1‑140 (MQ=255) cGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATg > 1:447198/1‑140 (MQ=255) cGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATg > 1:491234/1‑140 (MQ=255) cGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCCCTTGAGCGGAAAATg > 1:254033/1‑140 (MQ=255) aTTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAg > 1:26217/1‑140 (MQ=255) ttGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGc < 1:646773/140‑1 (MQ=255) ttGCTGCGCTTAATCGTCCAAATGAACTGTGGCATATGCCGGACTTAATCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGc < 2:185091/140‑1 (MQ=255) cgcTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGGAAAAATGGTCTGTCATGAGCGGAAAATGAGCATGgcg > 1:182802/1‑140 (MQ=255) cagCCAAAGAACTGTCCCATCTTCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACcgcg < 1:222787/138‑1 (MQ=255) tGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCgcgc > 1:517478/1‑140 (MQ=255) ccAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGATAAAATGCTGTCTGTTGGGCGGAAAAAGAGCATGGCGCAAACCGCGcac > 2:211378/1‑140 (MQ=255) gtgCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTg > 2:450794/1‑140 (MQ=255) tgCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTTTCTTGAGCAGAAAATGTGCATGGCGCAAACCGCGCACAGCCAGttctg > 2:222787/1‑137 (MQ=255) gCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAg < 2:137864/140‑1 (MQ=255) cATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAGCCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCa < 2:447198/140‑1 (MQ=255) aTCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAg < 2:491234/140‑1 (MQ=255) gACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCa < 1:538930/140‑1 (MQ=255) gccgccGAATGGCTGGACACCTACCAGGCGATAGATCTGCAAGCGACTAAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCaa < 2:182802/140‑1 (MQ=255) gccgccGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCaa < 1:597261/140‑1 (MQ=255) gccgccGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCaa < 2:26217/140‑1 (MQ=255) aaTGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCa < 2:638638/140‑1 (MQ=255) aaTGGCTGGAAACCTGCCAGGCTAATGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCa < 1:282337/140‑1 (MQ=255) cTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGtcgc > 1:554715/1‑140 (MQ=255) ggAAGCGACTGAAAACATGCTCTCTCTTGAGCGGAAACTGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGtcgctc < 1:206508/140‑1 (MQ=255) cGACTGAAAAAATGCGCTCTCTTGATCGGCAAATGAGTATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGaa < 1:211378/140‑1 (MQ=255) aCTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACt > 2:340373/1‑140 (MQ=255) cTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTa < 1:450794/140‑1 (MQ=255) cTGAAAAAATGCTCTCTCTTGAGCGGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTa < 2:554715/140‑1 (MQ=255) | CCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGACTTAACCGCCGACTGCGCCGCCGAATGGCTGGAAACCTTCCAGGCGAAAGAGCTGGAAGCGACTGAAAAAATGCTCTCTCTTGAGCAGAAAATGAGCATGGCGCAAACCGCGCACAGCCAGTTTGAGCAGGCTTATCAGCTGGTGGTGGCAATCAACGGCCCACTGGCGCGTAACGAGGCGTGGGATGTCGCTCGCGAACTA > NC_000913/977576‑977828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |