| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,048,572 | G→A | G40G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,048,572 | 0 | G | A | 98.0% | 159.4 / NA | 49 | G40G (GGC→GGT) | gfcC | capsule biosynthesis GfcC family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/36); minor base C (1/0); total (13/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGT > NC_000913/1048436‑1048694 | caagcTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCa < 2:337189/140‑1 (MQ=255) gtgCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGaa > 1:418782/1‑140 (MQ=255) tgCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGaaa < 2:600771/140‑1 (MQ=255) tgCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGaaa < 2:120515/140‑1 (MQ=255) cATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAgtg > 1:665224/1‑140 (MQ=255) gACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCt > 1:412145/1‑140 (MQ=255) aTGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCtgtt < 1:521790/140‑1 (MQ=255) tGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCtgttg > 2:97926/1‑140 (MQ=255) tGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCtgttg > 2:132510/1‑140 (MQ=255) gTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGc < 2:418782/140‑1 (MQ=255) gTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGc < 1:124999/140‑1 (MQ=255) tCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAgg < 1:28414/140‑1 (MQ=255) tCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAgg < 2:14117/140‑1 (MQ=255) tCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAgg < 1:660847/140‑1 (MQ=255) tCTTTAAGCGCTTTAGCCTTCGCCGCAGAGTCGGTAAGCCAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAgg < 2:205254/140‑1 (MQ=255) tttAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCa > 1:643135/1‑140 (MQ=255) tttAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACAGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCa > 2:551708/1‑140 (MQ=255) tAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGa > 1:621915/1‑140 (MQ=255) gcTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACAGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAAt < 1:551708/140‑1 (MQ=255) cTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:4856/1‑140 (MQ=255) cTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTATCCACGGGCCCAGCGGGAAGTGTCTGTTGTGCGCCAGGCAGATAAATa > 2:189647/1‑140 (MQ=255) tttAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAg < 1:593366/140‑1 (MQ=255) gccgcAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTcc < 2:131227/140‑1 (MQ=255) gcAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTg < 2:371767/140‑1 (MQ=255) agagTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgcg > 1:351737/1‑140 (MQ=255) agTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:632803/140‑1 (MQ=255) agTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 2:412145/140‑1 (MQ=255) agTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 2:643135/140‑1 (MQ=255) agTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 2:665221/140‑1 (MQ=255) agTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCACGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:294618/140‑1 (MQ=255) agTCGGTCAGCAAGGCACCAGGACACCAGAGTCGACCGCGCAGTTGCGCTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 2:179792/140‑1 (MQ=255) tCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:114043/140‑1 (MQ=255) aGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATg < 2:601270/140‑1 (MQ=255) cACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTc < 1:512603/140‑1 (MQ=255) cACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTc < 2:471280/140‑1 (MQ=255) aCCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa < 2:563015/140‑1 (MQ=255) aCCAGGCCACCAGAGGCCATCGTGCAGTTGCAGTTCTGTTACCAACTGAACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa < 2:200765/140‑1 (MQ=255) cAGGCCACCAGAGGCGATCGCGTAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAtt < 1:267898/140‑1 (MQ=255) ggCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTAc < 1:133721/140‑1 (MQ=255) gCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGc > 2:113695/1‑140 (MQ=255) aTCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTgg < 1:132510/140‑1 (MQ=255) gcgcAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCaa < 2:432893/140‑1 (MQ=255) tGCGGTTGTGTCACCTACTGGACAATATTCTCCACGGTACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTAATTACGTAAAGTACGCTGGCAATGAAATa < 1:189647/140‑1 (MQ=255) cGGTTGTGTCACCAACTAGACAGCATTTTCCTCGGAACCTACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACg < 2:301095/140‑1 (MQ=255) ggTTGTGTCACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGa < 1:97926/140‑1 (MQ=255) ttGTGTCACCAACTGGACAACATTTCCCAAGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACt < 1:269170/140‑1 (MQ=255) cACCAACTGGACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAAtt < 1:113695/140‑1 (MQ=255) gACAACATTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGCCATGGGGCGTCGTTACGTAATGTATGCTGGCAATGAAGTACGGCTGTAATTTATTCATgg > 2:175547/1‑140 (MQ=255) acaacaTTTTCCACGGGACCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGt < 2:665224/140‑1 (MQ=255) | CAAGCTGTGCCATGACATGTTGGTAGTCTTTAAGCGCTTTAGCTTTCGCCGCAGAGTCGGTCAGCAAGGCACCAGGCCACCAGAGGCGATCGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTGGCAATGAAATACGACTGTAATTTATTCATGGT > NC_000913/1048436‑1048694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |