Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,105,994 G→A D59N (GAT→AAT)  ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,105,9940GA96.9% 97.7 / NA 32D59N (GAT→AAT) ymdB2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/13);  minor base C (1/0);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCGATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAA  >  NC_000913/1105857‑1106127
                                                                                                                                         |                                                                                                                                     
ccAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAAt                                                                                                                                     >  1:29030/1‑140 (MQ=255)
ccAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAAt                                                                                                                                     >  1:397816/1‑140 (MQ=255)
     cTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGccc                                                                                                                                >  1:150936/1‑140 (MQ=255)
        gCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTAc                                                                                                                             >  1:625747/1‑140 (MQ=255)
        gCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTAc                                                                                                                             >  1:399169/1‑140 (MQ=255)
              gATGTGATTGTGAATGCGGCTAATCGGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCa                                                                                                                       <  2:58885/140‑1 (MQ=255)
              gATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGACAGGCAACAGAAGGGACATTGCCCCACGGGGCa                                                                                                                       >  2:318970/1‑140 (MQ=255)
                tgtgATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATg                                                                                                                     <  2:421868/140‑1 (MQ=255)
                         gAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGttatta                                                                                                            <  2:29030/140‑1 (MQ=255)
                                   aaTCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAgg                                                                                                  >  1:161916/1‑140 (MQ=255)
                                    aTCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGc                                                                                                 >  1:109448/1‑140 (MQ=255)
                                          cATTAATGGGAGGGGGCCGCGTAGATGGGGCGATTGATGTCGCAGCGGTTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCGGGGCATTTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCtt                                                                                           <  2:187963/140‑1 (MQ=255)
                                           aTTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTc                                                                                          >  1:641497/1‑140 (MQ=255)
                                                tGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCt                                                                                     >  1:135335/1‑140 (MQ=255)
                                                 gggAGGGGGCGGCGTCGATGGGGCCATTCATCGCCCGGCGGGCCCGGCCCTGCTGGATGTTTTTTAAAAAGTCAGGCAACAGCAGGGAAATGCCCCTGGGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTa                                                                                    <  2:482405/140‑1 (MQ=255)
                                                     ggcggcggcGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGc                                                                                >  1:643722/1‑140 (MQ=255)
                                                       gggcggcgTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCg                                                                              <  1:26811/139‑1 (MQ=255)
                                                       cggcggcgTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCg                                                                              <  2:109448/140‑1 (MQ=255)
                                                             cgTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGGATGCCGTTGTTAGGCTTGCAGGCGATCTTCGCGCTAAAGCCGTAGTGc                                                                        >  2:194519/1‑140 (MQ=255)
                                                             cgTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGc                                                                        >  1:477122/1‑140 (MQ=255)
                                                              gTCGATGGGGCCATTCATCGCGGAGGGGGTCCGGCCCTGGTGGATGCTTGTTTAAAAGTCAGGAAACAGCAGGGAAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGca                                                                       <  2:135335/140‑1 (MQ=255)
                                                                                cgcgCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTg                                                     >  1:140696/1‑140 (MQ=255)
                                                                                   gcggggggCCCGGCCCTGTTGGATGTTTGTTAAAAAGTCAGGCAACAGCGGGGAAATTGCCCTCCGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCg                                                  <  2:161916/135‑1 (MQ=255)
                                                                                   gcAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCg                                                  <  2:643722/140‑1 (MQ=255)
                                                                                   gcAGCGGGTCCGGCCCGCTTGGGTGTTTGTTAACACGTCCGGCAACAGGAGGGCATTTGCCCTCGGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCg                                                  <  2:298025/140‑1 (MQ=255)
                                                                                   gcAGCGGGTCCGGCCATGCTGGTTGGTTGATTAAAAGACAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCg                                                  <  1:194519/140‑1 (MQ=255)
                                                                                   cgagggggTCCGGCCCTGCGGAAGGCTTGTTTAAAGGCCAGCCACCAGCAGGGCAATGGCCCTACGGGGTATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCg                                                  <  2:76351/135‑1 (MQ=255)
                                                                                     aGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCgtg                                                >  1:32207/1‑140 (MQ=255)
                                                                                                    gctgGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACg                                 >  2:645998/1‑140 (MQ=255)
                                                                                                     ctgGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACTCCGTCGGGCCAGTCGGGCGTGGTGGTGAACAAAACGa                                >  1:321038/1‑140 (MQ=255)
                                                                                                        gATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTCGGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGa                             <  2:397816/140‑1 (MQ=255)
                                                                                                            tttGTTTAAAAGTCAGGCAACAGCAGGGCAATTCCCCTGCGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAg                         <  2:140696/139‑1 (MQ=255)
                                                                                                               gTTTAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCtt                      >  2:374735/1‑140 (MQ=255)
                                                                                                                 ttAAAAGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCtttt                    <  1:374735/140‑1 (MQ=255)
                                                                                                                      aGTCAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAgg               <  2:32207/140‑1 (MQ=255)
                                                                                                                         cAGGCAACAGCAGGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATg            <  1:645998/140‑1 (MQ=255)
                                                                                                                                  gcagGGCAATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCa   >  1:22642/1‑140 (MQ=255)
                                                                                                                                   cagGGCAATTGCCCTGCTGGGCCTGCCGTTAGTACCCTTACAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCaa  <  2:176483/140‑1 (MQ=255)
                                                                                                                                   cagGACAATTGCCCTAGGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCaa  <  1:156046/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCGCGCAGCGGGTCCGGCCCTGCTGGATGCTTGTTTAAAAGTCAGGCAACAGCAGGGCGATTGCCCTACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAA  >  NC_000913/1105857‑1106127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑