Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,666	A→G	G92G (GGT→GGC)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,666	0	A	G	100.0%	128.2 / NA	38	G92G (GGT→GGC)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/10);  total (28/10)

TCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAAGTCA  >  NC_000913/1108538‑1108786                                                                                                                                 |                                                                                                                         tCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTc                                                                                                               <  1:403102/140‑1 (MQ=255)       gCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACg                                                                                                         >  1:517171/1‑140 (MQ=255)                    atTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAt                                                                                            <  2:137395/140‑1 (MQ=255)                     tttGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATTGGGATGCCTACCCGCTCGACACGTACTTTCCACCAtt                                                                                           >  1:298935/1‑140 (MQ=255)                      ttGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACAGCGGGATGGCTACACGTACGACCCGTACTTTCCACCAttt                                                                                          >  2:189662/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACTTCGGGAGGCCTACACGTTCCACACCTACTTTCCACCAttttt                                                                                        >  2:285536/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  1:406735/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  1:141568/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  1:462906/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  2:24919/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  2:416293/1‑140 (MQ=255)                        gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCAttttt                                                                                        >  2:510187/1‑140 (MQ=255)                           aTACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACATTCGACGCGTACTTTCCACCATTTTTTCa                                                                                     >  1:203543/1‑140 (MQ=255)                             aCAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAg                                                                                   >  1:157660/1‑140 (MQ=255)                                       cccAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGGCTACACGTACGAAACGCACTTTCCAGCATTCTTTCAAGGGATAGCGTa                                                                         >  2:185934/1‑140 (MQ=255)                                            gCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCg                                                                           >  1:587372/1‑133 (MQ=255)                                            gCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCg                                                                           <  2:587372/133‑1 (MQ=255)                                                 cTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCAt                                                               <  2:336674/140‑1 (MQ=255)                                                 cTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTAATTTGCACAATTTTTTCAAGGGATAGCGTAAAAAAAGCAt                                                               >  2:233965/1‑140 (MQ=255)                                                                       atTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGAtata                                         >  2:156502/1‑140 (MQ=255)                                                                       atTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGAtata                                         >  1:636582/1‑140 (MQ=255)                                                                       atTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCACGTAGGAAAAGTAGCCGGAtata                                         >  2:256864/1‑140 (MQ=255)                                                                       atTGCAGCATAATAAATTGCGGTAATGTCAGTAGGCGGGCGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATCTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGAtata                                         <  2:198584/140‑1 (MQ=255)                                                                               ataataAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCGCCATTTTTTCAAGGGATAGCGTAAAGAAAGCATTTAGGAAAAGTAGCCGGATATAACGaaaaa                                 >  1:242046/1‑140 (MQ=255)                                                                                 aataaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATa                               >  2:368325/1‑140 (MQ=255)                                                                                   taaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATAcc                             >  2:36239/1‑140 (MQ=255)                                                                                          cGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGc                      >  1:404864/1‑140 (MQ=255)                                                                                             tAATGTCAGTAGGGGGCTGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGa                   <  2:406735/140‑1 (MQ=255)                                                                                             tAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGa                   <  1:36239/140‑1 (MQ=255)                                                                                                tGTCAGTAGGGGGATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACg                <  1:416293/140‑1 (MQ=255)                                                                                                         ggggGTGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGAGATAACGAAAAATACATGCATGAGGAACGAGTGGATGa       >  1:190132/1‑140 (MQ=255)                                                                                                         ggggATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGa       >  2:644587/1‑140 (MQ=255)                                                                                                         ggggATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGa       >  1:635009/1‑140 (MQ=255)                                                                                                          ggggTGGCTGTTAACATTGGGATGCCTACACTTTCTACATGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGaa      <  2:190132/140‑1 (MQ=255)                                                                                                            gATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCTCCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGa               <  1:574060/129‑1 (MQ=255)                                                                                                            gATGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGa               >  2:574060/1‑129 (MQ=255)                                                                                                              tGGCTGTTAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAAGTCa  >  2:16974/1‑140 (MQ=255)                                                                                                                    ttAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGa          >  2:366267/1‑126 (MQ=255)                                                                                                                    ttAACATCGGGATGCCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGa          <  1:366267/126‑1 (MQ=255)                                                                                                                                 |                                                                                                                         TCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAAGTCA  >  NC_000913/1108538‑1108786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑