Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,066	C→T	A149V (GCC→GTC)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,066	0	C	T	100.0%	102.1 / NA	31	A149V (GCC→GTC)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

TGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCG  >  NC_000913/1147932‑1148200                                                                                                                                       |                                                                                                                                        tGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACg                                                                                                                                    >  2:92276/1‑140 (MQ=255) tGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACg                                                                                                                                    >  2:594101/1‑140 (MQ=255)   ccGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTc                                                                                                                                  >  2:361188/1‑140 (MQ=255)   ccGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTc                                                                                                                                  >  2:336284/1‑140 (MQ=255)     ggTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGa                                                                                                                                >  1:152814/1‑140 (MQ=255)      gTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGAt                                                                                                                               <  1:55279/140‑1 (MQ=255)                 gcgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑Gcac                                                                                                                   >  2:17447/1‑140 (MQ=255)                    cTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAAt                                                                                                                >  1:167527/1‑140 (MQ=255)                         ggggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTgg                                                                                                           <  2:548616/140‑1 (MQ=255)                         ggggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTgg                                                                                                           <  2:167527/140‑1 (MQ=255)                                         ttaCTCAAGCCCCTGGAAGGGACTGAGCGTCAGGCACTGATGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTAt                                                                                           <  2:193985/140‑1 (MQ=255)                                                 gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCAtt                                                                                             <  1:455882/130‑1 (MQ=255)                                                 gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCAtt                                                                                             >  2:455882/1‑130 (MQ=255)                                                           gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGa                                                                         >  1:405108/1‑140 (MQ=255)                                                                  aGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtg                                                                  >  2:472961/1‑140 (MQ=255)                                                                  aGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtg                                                                  >  2:106823/1‑140 (MQ=255)                                                                  aGCGTCCGGCGCTGGTGACGGTATT‑CCAACTCAGCAAAAGGGCCAAACGGTGGTCCTTGGATTAGGGGACAACGTCGGTTGTGACACGCA‑AAAGGTGGGGGAATGTGCGCATATGGGTTTAGATTTGGCTGAAAAggcgg                                                                 >  2:189572/1‑140 (MQ=255)                                                                       ccGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGa                                                             <  1:361188/140‑1 (MQ=255)                                                                                               caTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGtt                                     <  1:662423/140‑1 (MQ=255)                                                                                                aTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTg                                    <  1:416718/140‑1 (MQ=255)                                                                                                 tCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTcgcg                                            <  2:347610/131‑1 (MQ=255)                                                                                                 tCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGc                                   >  1:251765/1‑140 (MQ=255)                                                                                                 tCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGAGGTGGAAATTCCCAATCCTcgcg                                            >  1:347610/1‑131 (MQ=255)                                                                                                  cAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCt                                  <  1:106823/140‑1 (MQ=255)                                                                                                  cAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCt                                  <  2:110796/140‑1 (MQ=255)                                                                                                  cAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCt                                  <  1:92276/140‑1 (MQ=255)                                                                                                               aaaCGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTgaa                     >  2:67890/1‑140 (MQ=255)                                                                                                                 aCGGTGGTCCTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTgaaga                   <  1:448130/140‑1 (MQ=255)                                                                                                                         ccTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTaa           <  1:593680/140‑1 (MQ=255)                                                                                                                          cTTGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTAGCGTGGCGTTGATCAATATTGGTGAAGAAGAAGTaaa          >  1:300726/1‑140 (MQ=255)                                                                                                                           ttGACTTAGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAg         <  1:521515/140‑1 (MQ=255)                                                                                                                                  aGGGGTCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCg  >  1:57529/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACA‑GCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGAAATTCCCAATCCTCGCGTGGCGTTGCTCAATATTGGTGAAGAAGAAGTAAAGGGTCTCG  >  NC_000913/1147932‑1148200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑