Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,155,557	T→C	V145A (GTT→GCT)	tmk →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,155,557	0	T	C	100.0%	91.4 / NA	27	V145A (GTT→GCT)	tmk	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/10);  total (17/10)

TCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCC  >  NC_000913/1155438‑1155690                                                                                                                        |                                                                                                                                      tCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTg                                                                                                                   >  1:66272/1‑140 (MQ=255)   aGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTgcg                                                                                                                 >  2:584343/1‑140 (MQ=255)       gTATCAGGGCGGCGGACGTGGTGTTGACCAAGATACGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGcgcgcg                                                                                                             <  2:226106/140‑1 (MQ=255)       gTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGcgcgcg                                                                                                             <  2:624964/140‑1 (MQ=255)            aGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAg                                                                                                        <  2:656321/140‑1 (MQ=255)                 ggcggACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGa                                                                                                   >  1:630310/1‑140 (MQ=255)                                      cATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCttt                                                                              >  1:367565/1‑140 (MQ=255)                                        tatGCTGGCAACACTGCCTGATACTGTTCTCCGGGATTTCCGCCCCGAGTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCg                                                                            <  2:219118/140‑1 (MQ=255)                                                cAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGTGCTGGATAGTATTGGGCAAGAATCTTTCGATTTCttt                                                                    >  1:219118/1‑140 (MQ=255)                                                cAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCg                                                                            <  2:103478/132‑1 (MQ=255)                                                cAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCg                                                                            >  1:103478/1‑132 (MQ=255)                                                cAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCttt                                                                    >  1:88583/1‑140 (MQ=255)                                                 aaCACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTa                                                                   >  2:510877/1‑140 (MQ=255)                                                        cGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGGTCGTATTGAGCAAGAAACTTTGGATTTCTTTAATCGCAc                                                            >  2:278787/1‑140 (MQ=255)                                                        cGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCAc                                                            >  1:64338/1‑140 (MQ=255)                                                        cGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCAc                                                            <  2:66272/140‑1 (MQ=255)                                                         gTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGGGAGCTGGAACGTATTGTGCAAGAATGTTTCCATTTCTTTAATCGCAcc                                                           >  2:247946/1‑140 (MQ=255)                                                             tGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCcgcg                                                       >  2:588293/1‑140 (MQ=255)                                                                    ctcGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTa                                                >  2:334287/1‑140 (MQ=255)                                                                     tcGGGGATTTTCGCCGCGACTTGACGCTCCATCTCGACGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTAt                                               <  1:278787/140‑1 (MQ=255)                                                                       ggggATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCt                                             >  2:559878/1‑140 (MQ=255)                                                                       ggggATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCt                                             >  2:452984/1‑140 (MQ=255)                                                                                      gACTTAACGCTCTATCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGAGTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGcaca                              >  2:436897/1‑140 (MQ=255)                                                                                                   aTCTCGATGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCAtt                 <  1:431830/140‑1 (MQ=255)                                                                                                          tGTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCa          <  2:71460/140‑1 (MQ=255)                                                                                                           gTTACCCCGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCAt         <  2:630310/140‑1 (MQ=255)                                                                                                                  cGGAAGCTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACAGGCAGCAAAAGGTAGAAGCATGCATACCATTGATGcc  >  1:203465/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                      TCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCC  >  NC_000913/1155438‑1155690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑