Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,172,093	A→G	R624R (CGT→CGC)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,172,093	0	A	G	100.0%	73.8 / NA	23	R624R (CGT→CGC)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

GCTGCGCGAGAAGGGTGGTAGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAG  >  NC_000913/1171975‑1172225                                                                                                                       |                                                                                                                                     gctgcGCGAGAAGGGTGGTAGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTCCCAAAGCCTACATCGCTGCACACCAGGCGATCCATTGCCAGCGGCTGa                                                                                                                 >  2:280420/1‑140 (MQ=255)  ctgcGCGAGAAGGGTGGTAGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGac                                                                                                                >  2:280311/1‑140 (MQ=255)       cgAGAAGGGTGGTAGGCAGCAGCACCGCCTCCTGTTTGTGGTTATCTACTGTCAGGAAAGCTTCCCGCATCGCCGCTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATg                                                                                                           <  2:572649/140‑1 (MQ=255)       cgAGAAGGGTGGTAGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATg                                                                                                           <  1:520475/140‑1 (MQ=255)       cgAGAAGGATGGTAGGATCCAGTACCGCCAACTCCGTGTAGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATg                                                                                                           <  2:321572/140‑1 (MQ=255)                    aGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCg                                                                                              <  1:503355/140‑1 (MQ=255)                          cAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTaa                                                                                        >  2:155618/1‑140 (MQ=255)                                                ttATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGt                                                                  >  1:533404/1‑140 (MQ=255)                                                    cTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAACTACCGCAATAAGGGGCTGCGCGTGATCCGGCGTGGtt                                                              >  1:193691/1‑140 (MQ=255)                                                        tGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCaa                                                          >  1:640261/1‑140 (MQ=255)                                                         gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGc                                                                    >  1:34739/1‑129 (MQ=255)                                                         gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGc                                                                    <  2:34739/129‑1 (MQ=255)                                                                    tGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTgc                                              >  1:558621/1‑139 (MQ=255)                                                                    tGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGt                                              >  1:651916/1‑140 (MQ=255)                                                                     gcgcgcATCGCCACTTCTGTTTTACCAAAGCTATCATCGCCGCACACCCGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTc                                             <  2:290919/140‑1 (MQ=255)                                                                          cATCGCCACTTCTGTTTTACCAAAGCCAATATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGa                                        <  1:280311/140‑1 (MQ=255)                                                                          cATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGa                                        <  1:155618/140‑1 (MQ=255)                                                                                    tCTGTTTTACCAAAGCCAACGTCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATa                              <  1:539278/140‑1 (MQ=255)                                                                                    tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATa                              <  2:558621/140‑1 (MQ=255)                                                                                              cAAAGCCAACATCGCCGGACACAAGGCGATCGATTACCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGa                    <  2:242579/140‑1 (MQ=255)                                                                                                  gCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCgt                >  1:54833/1‑140 (MQ=255)                                                                                                  gCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCgt                >  2:350552/1‑140 (MQ=255)                                                                                                  gCCAACATCGCCGCACACCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCgt                >  2:498045/1‑140 (MQ=255)                                                                                                                acacCAGGCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAg  <  2:651916/140‑1 (MQ=255)                                                                                                                       |                                                                                                                                     GCTGCGCGAGAAGGGTGGTAGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAG  >  NC_000913/1171975‑1172225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑