Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,185,933	C→T	G30G (GGC→GGT)	pepT →	peptidase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,185,933	0	C	T	100.0%	124.1 / NA	39	G30G (GGC→GGT)	pepT	peptidase T
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/16);  total (23/16)

TTCTTGCTTCTTAATAATGTTGTCACAAAAAGTGAGGGTGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTT  >  NC_000913/1185799‑1186068                                                                                                                                       |                                                                                                                                        ttCTTGCTTCTTAATAATGTTGTCACAAAAAGTGAGGGTGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATg                                                                                                                                    >  2:73669/1‑140 (MQ=255)                     tgtCACAAAAAGTGAGGGTGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGa                                                                                                                >  1:658269/1‑140 (MQ=255)                     tgtCACAAAAAGTGAGGGTGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGa                                                                                                                >  2:594471/1‑140 (MQ=255)                                  gAGGGTGACTACATGGATAAACGACTTGAGCGATTTTTGAACTGCGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGa                                                                                                   <  2:372652/140‑1 (MQ=255)                                       tGACTACATGGATAAACTACTTGAGCGATTTTTTATCTATGTGTCTCTTGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAgaga                                                                                              <  2:314109/140‑1 (MQ=255)                                       tGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAgaga                                                                                              <  2:83505/140‑1 (MQ=255)                                         aCTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATg                                                                                            >  1:368989/1‑140 (MQ=255)                                         aCTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATg                                                                                            >  1:447052/1‑140 (MQ=255)                                                ggATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTa                                                                                     >  2:611951/1‑140 (MQ=255)                                                 gATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTAt                                                                                    <  1:395311/140‑1 (MQ=255)                                                   tAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCa                                                                                  >  2:397091/1‑140 (MQ=255)                                                     aaCTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAAt                                                                                <  2:457238/140‑1 (MQ=255)                                                     aaCTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAAt                                                                                <  1:430055/140‑1 (MQ=255)                                                       ctactTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAAtgt                                                                              >  1:488298/1‑140 (MQ=255)                                                          ctTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGAc                                                                           <  1:611951/140‑1 (MQ=255)                                                            tGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCt                                                                         <  2:450306/140‑1 (MQ=255)                                                            tGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCt                                                                         >  1:154757/1‑140 (MQ=255)                                                            tGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCt                                                                         <  2:154757/140‑1 (MQ=255)                                                             gAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCGTCTGCTGAAAGAGCAGGTCGAAGAGGTGGGTCTTATCAATGTGACCtt                                                                        >  2:198891/1‑140 (MQ=255)                                                                      ttGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGaa                                                               <  2:658269/140‑1 (MQ=255)                                                                                            cccAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTa                                         >  2:496398/1‑140 (MQ=255)                                                                                             ccAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTAACCTTAAGTGAGAAGGGCACTATGATGGCGACGTTAc                                        >  1:187135/1‑140 (MQ=255)                                                                                             ccAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGATTATCAATATGAACTTGAGTGAGAAGGGCAGTTTGATGGCGACGTTAc                                        >  2:176316/1‑140 (MQ=255)                                                                                                aTCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCgg                                     >  1:553608/1‑140 (MQ=255)                                                                                                aTCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCgg                                     >  2:642019/1‑140 (MQ=255)                                                                                                aTCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCgg                                     >  2:456374/1‑140 (MQ=255)                                                                                                    aaaGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTaa                                 <  1:642019/140‑1 (MQ=255)                                                                                                    aaaGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTaa                                 <  1:569942/140‑1 (MQ=255)                                                                                                                agaCAGGTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGa                     >  2:620597/1‑140 (MQ=255)                                                                                                                     ggTTCCCAGCACGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATcc                >  1:72272/1‑140 (MQ=255)                                                                                                                      gTTCCCAGCACGGAATGTCAATCGACGTTCTTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATccc               <  1:176316/140‑1 (MQ=255)                                                                                                                            aGCACGGAAGGTCAATGGAAGTCATTGCAACTGCTGACAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGAt         <  1:198891/140‑1 (MQ=255)                                                                                                                            aGCACGGAAGGTCAATGCAAGTCATTGCATCCGCTTAAACAGCAGCTCTAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGAt         <  2:187135/140‑1 (MQ=255)                                                                                                                               aCGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTgg      <  1:456374/140‑1 (MQ=255)                                                                                                                                cGGAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTTATGGCGACGTTAACGGCTAACGTCCCTGGCGATATCCCGGCGATTGGc     >  2:175591/1‑140 (MQ=255)                                                                                                                                 ggAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGCTGGCGACGGTACAGGCAAACGTCCCTGGCGATATCCCGGCGATTGGCt    >  2:247458/1‑140 (MQ=255)                                                                                                                                 ggAAGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCt    >  2:3844/1‑140 (MQ=255)                                                                                                                                   aaGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCttt  >  1:505554/1‑140 (MQ=255)                                                                                                                                   aaGGTCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCttt  >  1:407076/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCTTGCTTCTTAATAATGTTGTCACAAAAAGTGAGGGTGACTACATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCAATCAAAAGCAGGGGTGAGACAGGTTCCCAGCACGGAAGGCCAATGGAAGTTATTGCATCTGCTGAAAGAGCAGCTCGAAGAGATGGGGCTTATCAATGTGACCTTAAGTGAGAAGGGCACTTTGATGGCGACGTTACCGGCTAACGTCCCTGGCGATATCCCGGCGATTGGCTTT  >  NC_000913/1185799‑1186068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑