Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,488	A→G	G46G (GGA→GGG)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,488	0	A	G	100.0%	75.9 / NA	23	G46G (GGA→GGG)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/7);  total (16/7)

GCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTC  >  NC_000913/71357‑71621                                                                                                                                    |                                                                                                                                      gCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTAACCGGTACGGTGATGGTGGCGGGGCTGGGGGCGCTGAt                                                                                                                               >  1:215343/1‑140 (MQ=255)          gAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAgcgg                                                                                                                      <  2:550972/140‑1 (MQ=255)          gAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAgcgg                                                                                                                      <  2:5463/140‑1 (MQ=255)                          aaTCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGGTTGGCAGCGGCGGGTTAAGATTCTgg                                                                                                      >  2:453341/1‑140 (MQ=255)                            tCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTTATTTGCCGCGGCGTGGTAAGTTTCttggc                                                                                                    >  1:271766/1‑136 (MQ=255)                            tCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGGGTTAAGTTTCTGGCa                                                                                                    >  1:268555/1‑140 (MQ=255)                            tCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGGTAAGTTTCTGGCa                                                                                                    >  1:79690/1‑140 (MQ=255)                                  gtgtATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGGTGATTGGCAGCTGCTGGGTAACTTTTTGGCACGCctg                                                                                              >  1:315280/1‑140 (MQ=255)                                     tATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGGGCTGATTGGCAGCGGCGAGGTTAGTTTTTGGCCCGCctggcg                                                                                           >  2:622783/1‑139 (MQ=255)                                      aTTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTCGGGGGGCTGATTGGCACGGGCGAGGATAGTATCTGGGACGCctggctg                                                                                          >  2:278082/1‑140 (MQ=255)                                          aTTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAg                                                                                      >  1:102708/1‑140 (MQ=255)                                             gATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGGTGGGGGCGGTGATTGGCAGCGGCGGGGTTAGTTTCTGGGCGGCCCGGCGGGCAgggg                                                                                   >  2:515102/1‑139 (MQ=255)                                               tGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGCTGGCGGGGGTGGGGGGGGTAATTGGGAGAGGCTGGTCTTGCTTCTAGCCGACCTGGCTGGCAGGGatt                                                                                 >  2:204357/1‑140 (MQ=255)                                                     tggtgTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGGGTTAAGTTTTTGGCGCGCCTGGGTTGCAGGGGTTATTggg                                                                           >  2:548700/1‑139 (MQ=255)                                                                 ccTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGc                                                               >  1:545996/1‑140 (MQ=255)                                                                 ccTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGGTTCTGGCACGCCTGGCTGGCAGGGGTTATTGGCTGCTTGGTgggg                                                               >  2:353725/1‑139 (MQ=255)                                                                 ccTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGGTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATgggg                                                               >  2:616765/1‑139 (MQ=255)                                                                   tttCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGa                                                             <  2:533425/140‑1 (MQ=255)                                                                                   cgctggTCGGTTTGATTCTACCTGGTACGGTTCTGTTGGTGGGGCTGGTGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTctgg                                             <  2:215343/140‑1 (MQ=255)                                                                                   cgctggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTctgg                                             <  2:545996/140‑1 (MQ=255)                                                                                              ttGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCg                                  <  2:116629/140‑1 (MQ=255)                                                                                                  ttCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGtttt                              >  1:478890/1‑140 (MQ=255)                                                                                                                 tGCTGATGGCGGGGCTGGGGGGGGTGTTTGGCAGCGGCGAGTTTAGTTTCCGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGGTGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCAt               >  2:479545/1‑140 (MQ=255)                                                                                                                 tGCTGATGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCAt               >  1:381507/1‑140 (MQ=255)                                                                                                                      aTGGCGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTg          >  2:79585/1‑140 (MQ=255)                                                                                                                              gCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTc  <  1:79585/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTC  >  NC_000913/71357‑71621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑