| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,230,806 | A→G | T14A (ACT→GCT) | umuD → | DNA polymerase V protein UmuD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,230,806 | 0 | A | G | 96.1% | 171.6 / ‑4.5 | 51 | T14A (ACT→GCT) | umuD | DNA polymerase V protein UmuD |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (32/17); total (33/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT > NC_000913/1230670‑1230940 | tATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCtt < 2:560848/140‑1 (MQ=255) aGCGACAAATATTGATAGCCTGCATCAGTTTTGATCTACTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCg < 1:314452/140‑1 (MQ=255) cGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCt < 1:49272/140‑1 (MQ=255) gACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTa > 2:436999/1‑140 (MQ=255) aaTATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTa > 2:523823/1‑140 (MQ=255) atatTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAg < 2:615257/140‑1 (MQ=255) aGCCTGAATCAGTATTGATCTGCTGGCAAGATCAGTCTAATGTATATATAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTg < 2:207331/140‑1 (MQ=255) gCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGt > 2:82740/1‑140 (MQ=255) tATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCt > 2:338504/1‑140 (MQ=255) ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCAcc > 2:93040/1‑140 (MQ=255) tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGa > 1:518807/1‑140 (MQ=255) ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCGCCAGCAGCACATTACGTTGAACAgc > 1:314806/1‑140 (MQ=255) aaaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcgc > 1:344122/1‑140 (MQ=255) aaaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgcgc > 2:491907/1‑140 (MQ=255) aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCa < 1:436999/140‑1 (MQ=255) aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt > 2:63358/1‑140 (MQ=255) aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt < 2:518807/140‑1 (MQ=255) cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa > 1:505128/1‑140 (MQ=255) cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa > 1:605230/1‑140 (MQ=255) cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa > 2:408760/1‑140 (MQ=255) gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc < 1:338504/140‑1 (MQ=255) gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc > 2:577313/1‑140 (MQ=255) gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc > 2:460867/1‑140 (MQ=255) tataACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt < 1:460867/140‑1 (MQ=255) ttCAGGCAGATTATTATTTTGTCTATCAAGCCTGCTGATATCCGCGAACTTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCa < 2:199048/140‑1 (MQ=255) ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGTAGATTACATTGTACGGCGCATTGATCTGAATCAACTg > 1:192862/1‑140 (MQ=255) ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg > 2:165598/1‑140 (MQ=255) ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg > 2:137325/1‑140 (MQ=255) ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg > 2:663547/1‑140 (MQ=255) ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCCGAATCAACTg > 1:606603/1‑140 (MQ=255) ggCAGATTATTATGTTGCTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg > 2:47568/1‑140 (MQ=255) attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCa > 2:619977/1‑131 (MQ=255) attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCa < 1:619977/131‑1 (MQ=255) tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc > 1:541077/1‑140 (MQ=255) tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc > 1:335048/1‑140 (MQ=255) tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc > 1:565802/1‑140 (MQ=255) tttATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg > 1:577228/1‑140 (MQ=255) ttATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgc < 2:442804/140‑1 (MQ=255) tATCAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGAGCAACGATCTGAATCAGCTGTCGATCCAGCATCCCAgcg > 2:271808/1‑140 (MQ=255) cAAGCCTGCGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGa < 1:165598/140‑1 (MQ=255) cGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc > 1:528424/1‑140 (MQ=255) cGGATCTCCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTCCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc > 2:131116/1‑140 (MQ=255) cGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTCCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc > 2:501899/1‑140 (MQ=255) aTCTCCGCGAAATTGTGACTTTACCGCTATTTAGCAATCATGTTCAGTATGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc < 2:169973/140‑1 (MQ=255) tctcCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCa < 2:528424/140‑1 (MQ=255) ctcCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc > 1:515153/1‑135 (MQ=255) ctcCGCGAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc < 2:515153/135‑1 (MQ=255) cgAAATTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCaa > 2:372225/1‑140 (MQ=255) aaaTTGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgt > 2:348321/1‑140 (MQ=255) ttGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt < 2:605230/140‑1 (MQ=255) ttGTGGCTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt < 1:491907/140‑1 (MQ=255) | TATGCAGCGACAAATATTGATAGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGT > NC_000913/1230670‑1230940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |