Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,660	T→C	V532A (GTT→GCT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,660	0	T	C	100.0%	132.7 / NA	38	V532A (GTT→GCT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

ATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGG  >  NC_000913/1252526‑1252782                                                                                                                                       |                                                                                                                           atTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGcttctt                                                                                                                       <  1:637723/140‑1 (MQ=255)    tGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTCCGCGGCTGGAAATTGACGACGATGCACTCGGTCGCCTGGCTTCTtgtg                                                                                                                    >  2:242287/1‑140 (MQ=255)           aCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGcc                                                                                                             >  1:379480/1‑140 (MQ=255)           aCCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGcc                                                                                                             >  1:89662/1‑140 (MQ=255)                      tctGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGAtt                                                                                                  >  2:387700/1‑140 (MQ=255)                       ctGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGAttt                                                                                                 >  1:442998/1‑140 (MQ=255)                          cGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTGGCCTGGCATCTAGTCCATGGCAAGGCAACGATTTTGa                                                                                              >  1:245903/1‑140 (MQ=255)                          cGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTg                                                                                               >  2:332523/1‑139 (MQ=255)                          cGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTg                                                                                               <  1:332523/139‑1 (MQ=255)                                    gTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGc                                                                                    >  2:101732/1‑140 (MQ=255)                                            aTTCCGGCGCTGGTGAATAACACATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCga                                                                            <  2:204129/140‑1 (MQ=255)                                            aTTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCga                                                                            <  2:379480/140‑1 (MQ=255)                                            aTTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCga                                                                            <  1:101732/140‑1 (MQ=255)                                                  gcgcTGGTGAATAACAAATTACGGAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCACTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCt                                                                      >  2:414977/1‑140 (MQ=255)                                                     cTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGc                                                                   <  1:514902/140‑1 (MQ=255)                                                      tGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGct                                                                  <  2:585740/140‑1 (MQ=255)                                                      tGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGct                                                                  <  2:38611/140‑1 (MQ=255)                                                      tGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGct                                                                  <  1:619501/140‑1 (MQ=255)                                                      tGGTGAATAACAAATTACGCAGGCTTGAAAAACGCTTTTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGct                                                                  <  1:304420/140‑1 (MQ=255)                                                          gAATAACAAATTACGCATTCTTGAAAAACGCTTCTCTACCCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGa                                                              <  2:316203/140‑1 (MQ=255)                                                           aaTAACAAATTACGCTGTCTTGCAACACGCTTCTCTATGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGag                                                             <  1:319456/140‑1 (MQ=255)                                                                   aTTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATa                                                     <  2:442998/140‑1 (MQ=255)                                                                     tACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAAc                                                   >  1:438061/1‑140 (MQ=255)                                                                       cGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTATGAACTTTTCAGAGTCTTCGAGAATTTTGCTCTGAGTAGTGATAACgg                                                 >  2:230743/1‑140 (MQ=255)                                                                          aGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCTTCATCGAGAATGTTGCTCTGAGTAGTGATAACGGgcg                                              >  1:174222/1‑140 (MQ=255)                                                                              ttGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCAtt                                          >  1:79262/1‑140 (MQ=255)                                                                               tGAAAAGCGCTTCTCGACGCGGTTGTAAATTGATGACCATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTc                                         <  1:278276/140‑1 (MQ=255)                                                                                      cGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAg                                  >  1:101335/1‑140 (MQ=255)                                                                                      cGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAg                                  >  2:42309/1‑140 (MQ=255)                                                                                      cGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAg                                  >  1:26309/1‑140 (MQ=255)                                                                                      cGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAg                                  >  1:404471/1‑140 (MQ=255)                                                                                           agctACGCGGCTGAAAATTGATGACGATGCCTTCGGTCGTCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGAtt                             <  2:229624/138‑1 (MQ=255)                                                                                                   ggCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGaa                     <  2:334082/140‑1 (MQ=255)                                                                                                   ggCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGaa                     >  1:440023/1‑140 (MQ=255)                                                                                                     cTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACa                   >  1:359513/1‑140 (MQ=255)                                                                                                     cTGAAAATTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACa                   >  2:502279/1‑140 (MQ=255)                                                                                                        aaaaTTGATGACGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGAATTCGCGTCAGTGATTTGCCGGAACATCt                >  1:229624/1‑140 (MQ=255)                                                                                                               atgaCGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTAcc         >  1:105183/1‑140 (MQ=255)                                                                                                                   cGATGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGc     <  1:372678/140‑1 (MQ=255)                                                                                                                      tGCCCTCGCTCGCCTGGCTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAgg  >  1:491073/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                           ATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGG  >  NC_000913/1252526‑1252782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑