Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,105	A→G	V6A (GTC→GCC)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,105	0	A	G	100.0%	116.1 / NA	35	V6A (GTC→GCC)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

AGTGGGATTCGAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGACATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCG  >  NC_000913/1297976‑1298232                                                                                                                                  |                                                                                                                                aGTGGGATTCGAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAAc                                                                                                                       <  1:140532/140‑1 (MQ=255)     ggATTCGAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGcc                                                                                                                   <  2:103099/140‑1 (MQ=255)      gATTCGAGCATCTGCCGCAGCCTAAACGGGGGCGCGGGAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCa                                                                                                                  <  1:207680/140‑1 (MQ=255)           gAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATg                                                                                                             <  2:583999/140‑1 (MQ=255)           gAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATg                                                                                                             <  2:624528/140‑1 (MQ=255)            aGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGTGTGCGTTCAGTTCAGCGGCATTAGTAACAGTCATAATGc                                                                                                            >  2:297079/1‑140 (MQ=255)            aGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGc                                                                                                            >  2:472210/1‑140 (MQ=255)             gCATCTGCAGCAGCCCGAGCGGTGGGGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGct                                                                                                           <  1:264420/140‑1 (MQ=255)                tCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTATACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCtctc                                                                                                        >  2:291334/1‑140 (MQ=255)                        agcCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAAt                                                                                                <  2:54911/140‑1 (MQ=255)                                                   gTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGc                                                                     <  1:472210/140‑1 (MQ=255)                                                          tGCTCTTGAGTGAAAGTGGCATATTCACGGTGGGCTTGTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCTTGATAATGTTAAACTTTTTTAGTAAAACATCTGCTCGAATa                                                              <  1:297079/140‑1 (MQ=255)                                                            ctctTGAGTGAAACTGGCATATTCAGGCTAGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACg                                                            <  1:240751/140‑1 (MQ=255)                                                            ctctTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACg                                                            <  1:291334/140‑1 (MQ=255)                                                            ctctTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACg                                                            <  2:631841/140‑1 (MQ=255)                                                            ctctTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACg                                                            <  2:19077/140‑1 (MQ=255)                                                            ctctTGAGTGAAACTAGCATATCCAAGCTTGGCTTTTCTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACg                                                            <  1:321595/140‑1 (MQ=255)                                                                  aGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTACACTTTTTTAGTGAAGCATCTGCTCGAATACGagagaa                                                      >  1:315061/1‑138 (MQ=255)                                                                            gCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGc                                            >  2:570084/1‑140 (MQ=255)                                                                                    aCGCTGGGCTTTTTTCACACGCCCTGCGAATGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGtgatga                                    <  1:282624/140‑1 (MQ=255)                                                                                      gCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGAtt                                  >  1:39057/1‑140 (MQ=255)                                                                                       cTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGtg                                        <  2:108995/133‑1 (MQ=255)                                                                                       cTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGtg                                        >  1:108995/1‑133 (MQ=255)                                                                                          ggCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACCTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCt                              >  1:618608/1‑140 (MQ=255)                                                                                                    acacGCTCTACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGa                    >  2:95333/1‑140 (MQ=255)                                                                                                          tctACGAGTGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCa              >  1:147458/1‑140 (MQ=255)                                                                                                                  tGCGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTa      >  2:524567/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCTTTAGTTACAGCCATAATGTTCTCCTGATAATTTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    <  2:315061/140‑1 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATGGTCAGTGCCGTGATGATTTGCTTAACCTATTCAAATCAAGAGCTTACt    >  2:322599/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    >  2:34765/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    >  2:397286/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    >  1:580554/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    >  1:340365/1‑140 (MQ=255)                                                                                                                    cGTTAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACt    >  2:87432/1‑140 (MQ=255)                                                                                                                      ttAAGTTCAGCGGCATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGGGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTcg  >  2:619615/1‑140 (MQ=255)                                                                                                                      ttAAGTTCAGCGGCAGTAGTAAAAGACATAATGCTCTTCTTATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTcg  <  1:292695/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                AGTGGGATTCGAGCATCTGCAGCAGCCAGAGCGGCGGCGCGGAAGATTTTGTCTACTTGCTCTTGAGTGAAACTGGCATATTCACGCTGGGCTTTTTTTACACGCTCTACGAGTGCGTTAAGTTCAGCGACATTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCG  >  NC_000913/1297976‑1298232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑