Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,759	T→C	V193A (GTG→GCG)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,759	0	T	C	100.0%	198.1 / NA	57	V193A (GTG→GCG)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/31);  total (26/31)

ATGAAATTCTTGAAGGGAAAAAACCGATTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACC  >  NC_000913/1301624‑1301886                                                                                                                                        |                                                                                                                                atgaAATTCTTGAAGGGAAAAACCCCATTTCCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCa                                                                                                                             <  1:299017/140‑1 (MQ=255) atgaAATTCTTGAAGGGAAAAAACCGATTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCa                                                                                                                             <  2:416265/140‑1 (MQ=255)      aTTCTTGAAGGGAAAAAACCGATTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGc                                                                                                                        >  1:98415/1‑140 (MQ=255)                           aTTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGa                                                                                                   <  1:333775/140‑1 (MQ=255)                           aTTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGa                                                                                                   >  1:32419/1‑140 (MQ=255)                            ttACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGaa                                                                                                  >  2:570498/1‑140 (MQ=255)                                   tctcGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGa                                                                                           <  2:587420/140‑1 (MQ=255)                                    ctcGGCGTGAAAGCTATTGATGTTCGCACATTAGAAGACACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGAc                                                                                          <  1:325541/140‑1 (MQ=255)                                     tcGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGAcc                                                                                         <  2:98415/140‑1 (MQ=255)                                     tcGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGAcc                                                                                         <  2:345078/140‑1 (MQ=255)                                     tcGGCGTGAAAGCTATTAATGATCACACATTCGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGAcc                                                                                         <  2:226243/140‑1 (MQ=255)                                       ggCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCa                                                                                       <  2:542475/140‑1 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAGGCCGCTAGCGATAATTTCGGCCAAAAATGGACCCAGc                                                                                     >  1:201460/1‑140 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGc                                                                                     <  2:607657/140‑1 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGc                                                                                     <  2:378342/140‑1 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGc                                                                                     <  2:104028/140‑1 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCG‑AAAATGGACCCAGcc                                                                                    <  1:587947/140‑1 (MQ=255)                                         cGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGACGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGc                                                                                     >  2:452765/1‑140 (MQ=255)                                          gTGAAAGCTATTGATGTTCACACATTAGAAGACACATTACGTGAACCCCTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGcc                                                                                    <  2:324174/140‑1 (MQ=255)                                             aaaGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTgg                                                                                 >  1:20133/1‑140 (MQ=255)                                                       atgatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAg                                                                                      <  1:445402/125‑1 (MQ=255)                                                       atgatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAg                                                                                      >  2:445402/1‑125 (MQ=255)                                                          atCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATCCGGCGAATAATGGATCCAGCCTGGTAATATCGTCAcc                                                                    >  1:193950/1‑140 (MQ=255)                                                           tCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCa                                                                   >  1:90387/1‑140 (MQ=255)                                                            cacacaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCaa                                                                  >  1:568302/1‑140 (MQ=255)                                                             acacaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCG‑AAAATGGACCCAGCCTGGTAATATCGTCACCAACg                                                                <  2:555582/140‑1 (MQ=255)                                                              cacaTTAGAAGTCACTTTAAGTCAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAGATGGACCCAGCCTGGTAATATCGTCACCAACg                                                                <  1:175733/140‑1 (MQ=255)                                                              cacaTTAGAAGTCACGTTAAGTAAAACCGTTCCGTACATCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACg                                                                <  1:325712/140‑1 (MQ=255)                                                              cacaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACg                                                                >  2:47413/1‑140 (MQ=255)                                                                    aGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAACAATTGACTCAGCCTGGGAATATCGTCACCAACGGTGCCt                                                          >  2:234206/1‑140 (MQ=255)                                                                     gAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCta                                                         <  2:20133/140‑1 (MQ=255)                                                                         acACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCCTCAAAAGCCGCTATCGAGAAATTCGGGGAAAAATGAACCTAGCCTGGGAATATCGTCACCAACGGTGCCTATAcc                                                     >  2:279450/2‑140 (MQ=255)                                                                           aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCtt                                                   >  2:107542/1‑140 (MQ=255)                                                                                  gTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAt                                            <  1:47413/140‑1 (MQ=255)                                                                                     aaCCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAt                                            <  2:98210/137‑1 (MQ=255)                                                                                     aaCCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAt                                            >  1:98210/1‑137 (MQ=255)                                                                                          gTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCTTCACCAATGGTGCCTATACCTTAAAAGATTGGGTCGt                                    >  1:253122/1‑140 (MQ=255)                                                                                            tCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTaa                                  >  1:530659/1‑140 (MQ=255)                                                                                             ccGTACTTCTATAAATTCCTTGGTCACCCATAAACTTCAACGGCGCCATAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTaaa                                 <  2:212612/140‑1 (MQ=255)                                                                                             ccGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTaaa                                 <  1:25482/140‑1 (MQ=255)                                                                                                tACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCGACGGTGCATATACCTTAAAAGATTGGGTCGTAaacga                              >  1:299716/1‑140 (MQ=255)                                                                                                               cTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGa               >  1:116092/1‑140 (MQ=255)                                                                                                               cTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGa               >  1:530957/1‑140 (MQ=255)                                                                                                               cTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGa               >  2:133435/1‑140 (MQ=255)                                                                                                               cTTGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCGTAAAAGACTGGATCATAAACGTACGAATCGTTCTTGa               >  1:316298/1‑140 (MQ=255)                                                                                                                ttGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGaa              <  2:568302/140‑1 (MQ=255)                                                                                                                ttGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGaa              <  2:116092/140‑1 (MQ=255)                                                                                                                ttGTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGaa              >  2:53823/1‑140 (MQ=255)                                                                                                                  gTTCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACg            >  2:468663/1‑140 (MQ=255)                                                                                                                   ttCACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCGTATACCTTTAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAACGc           >  2:267430/1‑140 (MQ=255)                                                                                                                     cACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAaacgaacg                           <  2:635260/122‑1 (MQ=255)                                                                                                                     cACCCATCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAaacgaacg                           >  1:635260/1‑122 (MQ=255)                                                                                                                          aTCAACTTGACCGGCGCCAAAAGCCGCTATCGATAGATTCGGCGAAAACTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGa    <  2:299716/140‑1 (MQ=255)                                                                                                                          aTCAACTTCACCGGCGCCAAAATCCTCTATCGAGAAACTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGa    <  1:267430/140‑1 (MQ=255)                                                                                                                          aTCAACTTCACCGGCGCCAAAATCCGCTATCTAGATATTAGGCGAAAATTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGa    <  2:214982/140‑1 (MQ=255)                                                                                                                          aTCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGa    <  1:133435/140‑1 (MQ=255)                                                                                                                          aTCAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGa    <  1:621651/140‑1 (MQ=255)                                                                                                                            cAACTTCACCGGCGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGAcc  >  1:505282/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                ATGAAATTCTTGAAGGGAAAAAACCGATTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACC  >  NC_000913/1301624‑1301886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑