Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,394,303	A→G	E359E (GAA→GAG)	mppA →	murein tripeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,394,303	0	A	G	100.0%	85.6 / NA	25	E359E (GAA→GAG)	mppA	murein tripeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/12);  total (13/12)

TGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAACTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGATTGCTGTAGCAT  >  NC_000913/1394170‑1394424                                                                                                                                      |                                                                                                                          tGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTGACGCCGGACCCTTCGCCGTTTGAACCAATGGGTCAGGAAGAGCTGAAt                                                                                                                     >  2:291086/1‑140 (MQ=255)  gACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATg                                                                                                                    >  2:356100/1‑140 (MQ=255)   aCGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATgc                                                                                                                   >  2:334756/1‑140 (MQ=255)                        aCCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAg                                                                                              <  2:566028/140‑1 (MQ=255)                        aCCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAg                                                                                              <  2:134333/140‑1 (MQ=255)                         ccGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGGGGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAgc                                                                                             <  1:476354/140‑1 (MQ=255)                            aaaaaGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTttg                                                                                                   >  1:226283/1‑131 (MQ=255)                            aaaaaGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTttg                                                                                                   <  2:226283/131‑1 (MQ=255)                            aaaaaGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCa                                                                                          <  2:577647/140‑1 (MQ=255)                            aaaaaGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCa                                                                                          >  2:350343/1‑140 (MQ=255)                            aaaaaGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCa                                                                                          <  1:350343/140‑1 (MQ=255)                                       gggACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATgg                                                                               >  2:525758/1‑140 (MQ=255)                                            gggCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGc                                                                          >  1:398262/1‑140 (MQ=255)                                                        aGCGTGGCATTTTACACCAGATGTTACCGTGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAAGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGa                                                              >  2:106562/1‑140 (MQ=255)                                                            tGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCt                                                          <  1:455607/140‑1 (MQ=255)                                                              gCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGa                                                        >  1:337984/1‑140 (MQ=255)                                                                        ccAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGta                                              <  1:356100/140‑1 (MQ=255)                                                                        ccAGATGTTACCGCGCGATTTACGCGGGATCCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGta                                              <  1:234575/140‑1 (MQ=255)                                                                                   cgcgGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAGAAACCGCCGAAGCTGACGCTTCTGTATAACACTTCAg                                   >  1:242394/1‑140 (MQ=255)                                                                                     cgGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGaa                                 >  2:110563/1‑140 (MQ=255)                                                                                       ggATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGaaaa                               >  1:477828/1‑140 (MQ=255)                                                                                                  ggAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAAACGCTGAAGATGAGGCTATTGTATAACACTTGAGAAAACCATCaaaaaa                    >  2:203003/1‑140 (MQ=255)                                                                                                         tCGCCGTTTGAACAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGAt             <  2:444015/140‑1 (MQ=255)                                                                                                                   aaCAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGATTGCTGTAGCa   <  2:477828/140‑1 (MQ=255)                                                                                                                    aCAAATGAGTCAGGAAGAGCTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGATTGCTGTAGCAt  <  1:17774/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                          TGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTGGCATTTTACACCAGATGTTACCGCGGGATTTACGCCGGAACCTTCGCCGTTTGAACAAATGAGTCAGGAAGAACTGAATGCGCAGGCAAAAACTTTGTTGAGCGCAGCTGGTTATGGTCCGCAAAAACCGCTGAAGCTGACGCTTTTGTATAACACTTCAGAAAACCATCAAAAAATTGCGATTGCTGTAGCAT  >  NC_000913/1394170‑1394424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑