| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,671,708 | Δ1 bp | intergenic (+24/‑69) | asr → / → ydgU | acid shock protein/uncharacterized protein YdgU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,671,708 | 0 | G | . | 95.5% | 171.0 / 1.9 | 44 | intergenic (+24/‑69) | asr/ydgU | acid shock protein/uncharacterized protein YdgU |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (17/25); minor base C (0/2); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
ATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGC > NC_000913/1671572‑1671841 | aTACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCcccc > 1:173323/1‑140 (MQ=255) aCGAAAGCTGAACAGTAAGCCCCTGCACATAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCt < 1:272579/140‑1 (MQ=255) aCGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCt < 2:780744/140‑1 (MQ=255) aaaGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAGGTTTTCAACAGTAATGCTGGCGC‑CCCACTCGCGCCTAAAAATTa > 1:538071/1‑140 (MQ=255) aaaGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTa < 2:173323/140‑1 (MQ=255) cccTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTg > 1:829234/1‑140 (MQ=255) cccTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTg > 1:598170/1‑140 (MQ=255) tGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCCGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTa > 1:7412/1‑140 (MQ=255) gAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTaa > 1:810821/1‑140 (MQ=255) aaCAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAg > 1:119770/1‑140 (MQ=255) aaCAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAg > 1:187697/1‑140 (MQ=255) aaaaGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCggg < 2:169868/140‑1 (MQ=255) aaaGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCACCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGt > 1:155647/1‑140 (MQ=255) aaGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTa < 2:562352/140‑1 (MQ=255) aaGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTa < 2:841432/140‑1 (MQ=255) cGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCAcc < 2:187697/140‑1 (MQ=255) aaCACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCttt > 2:713439/1‑140 (MQ=255) cacGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAg < 2:598170/140‑1 (MQ=255) cGCCAAGAAACACAGCCACCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCg < 2:155647/140‑1 (MQ=255) ccAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa < 2:680896/140‑1 (MQ=255) ccAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa < 2:573767/140‑1 (MQ=255) ccAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa < 1:471903/140‑1 (MQ=255) acacAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCg > 1:690850/1‑131 (MQ=255) acacAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCg < 2:690850/131‑1 (MQ=255) cacaGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa > 1:89607/1‑132 (MQ=255) cacaGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGa < 2:89607/132‑1 (MQ=255) aTCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTGACGGTTACCACCGCCTTTAGCGGAGGGTAGTATGGTggg > 2:282989/1‑140 (MQ=255) ccGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATc > 1:774809/1‑140 (MQ=255) ccGGCAAAACCTACTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATc > 1:118112/1‑140 (MQ=255) cAAAACCTCCTGCACATCCCGCCGCGTAAGGATACTACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCtt < 2:410775/140‑1 (MQ=255) ccTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGt < 1:147183/140‑1 (MQ=255) ccTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGt < 2:515736/140‑1 (MQ=255) ccTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGt < 1:713439/140‑1 (MQ=255) aaCCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGGACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat < 2:829234/140‑1 (MQ=255) aaCCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGat < 1:654948/140‑1 (MQ=255) aCCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGatc > 1:89013/1‑140 (MQ=255) cccGCAAAGTACGTTTTCAACAGTAATCATGGCGC‑CACCCTCGCGACTGCAAATTATGGTGCTCAGCGGGTAACGTCTAGCACCGCCTTTAGCGGAGGGTAATATGGTGGGCCGTTATCGCTTTGAGCTCATTCTGatca < 2:538071/140‑1 (MQ=255) tttCAACAGTAATGCTGGCGC‑CCCCGTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATgc < 1:394027/140‑1 (MQ=255) ttCAACAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTGGAGTTCATACTGAGCATCCTTATTTTATgcg > 2:317767/1‑140 (MQ=255) cAACAGTAATGCTGGCGC‑CCCCCCCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCa < 2:810821/140‑1 (MQ=255) aaCAGTAATGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCAc < 2:764613/140‑1 (MQ=255) aaCAGTAATGCTGGC‑CCCCCCCGCGCGCCTGAAAATTTCGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCAc < 1:317767/140‑1 (MQ=255) aaCAGTAATGCTGGC‑CCCCCCCACGGGCCCGAAAATTTCGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCACTCTGATCATCCTTATTTTATGCGCAc < 1:282989/140‑1 (MQ=255) aTGCTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTAc > 2:445526/1‑140 (MQ=255) cTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGc < 2:7412/140‑1 (MQ=255) cTGGCGC‑CCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGc < 2:1033/140‑1 (MQ=255) | ATACGAAAGCTGAACAGAAAGCCCCTGAACAAAAAGCGCAGGCAGCGAAGAAACACGCCAAGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCGGAGGGTAGTATGGTGGGCCGTTATCGCTTTGAGTTCATTCTGATCATCCTTATTTTATGCGCACTGATTACCGC > NC_000913/1671572‑1671841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |