Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,705,751	(T)6→5	intergenic (+61/‑16)	ydgK → / → rsxA	conserved inner membrane protein YdgK/SoxR [2Fe‑2S] reducing system protein RsxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,705,746	0	T	.	100.0%	170.9 / NA	41	intergenic (+56/‑21)	ydgK/rsxA	conserved inner membrane protein YdgK/SoxR [2Fe‑2S] reducing system protein RsxA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/23);  total (18/23)

CTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGA  >  NC_000913/1705607‑1705881                                                                                                                                            |                                                                                                                                        cTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCt                                                                                                                                         >  1:45118/1‑140 (MQ=255)           tgttgGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCa                                                                                                                              >  2:517278/1‑140 (MQ=255)            gttgGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAg                                                                                                                             >  2:678919/1‑140 (MQ=255)              tgGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGc                                                                                                                           >  1:122078/1‑140 (MQ=255)                     cGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGaaaaa                                                                                                                    >  2:167263/1‑140 (MQ=255)                        aTTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAAtga                                                                                                                 <  2:135255/140‑1 (MQ=255)                        aTTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAAtga                                                                                                                 <  1:434665/140‑1 (MQ=255)                        aTTGATCGTCCCCTAGTTCAAAGGCACGTAGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAAtga                                                                                                                 <  1:296913/140‑1 (MQ=255)                            aTCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAAtgactga                                                                                                             <  2:182335/140‑1 (MQ=255)                            aTCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAAtgactga                                                                                                             <  1:87489/140‑1 (MQ=255)                                  cccTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCt                                                                                                       >  2:456628/1‑140 (MQ=255)                                              cGCTCGTCGCGGGTAAGCGCGACGTTTGTGAGTCCGTAATAACCTTACCGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  2:388821/140‑1 (MQ=255)                                              cGCTCGTCGCGGGTAAAAGCGAGGTTTGTGACTCCATAACAACCTTACCGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  2:319164/140‑1 (MQ=255)                                              cGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  1:167263/140‑1 (MQ=255)                                              cGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  1:191267/140‑1 (MQ=255)                                              cGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  1:810742/140‑1 (MQ=255)                                                 tcgtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAGATGACTTACTACCCGTTACTGTTTGTCgg                                                                                        >  1:275171/1‑140 (MQ=255)                                                  cgtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGATTGTCTACCTGTTACTGTTTGTCGGa                                                                                       >  1:388821/1‑140 (MQ=255)                                                   gtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           >  1:678061/1‑135 (MQ=255)                                                   gtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGt                                                                                           <  2:678061/135‑1 (MQ=255)                                                   gtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGaa                                                                                      <  1:2593/140‑1 (MQ=255)                                                   gtcgCGGGTAAAAGCGAAGTTTTTGAATCCGTAATAACCCTACAGTTACCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGaa                                                                                      <  2:275171/140‑1 (MQ=255)                                                         ggTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGGACTGTAc                                                                                <  1:517278/140‑1 (MQ=255)                                                         ggTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTAc                                                                                <  1:735853/140‑1 (MQ=255)                                                                gCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCaa                                                                         <  1:665822/140‑1 (MQ=255)                                                                gCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCaa                                                                         <  2:45118/140‑1 (MQ=255)                                                                gCGACGTTTGTGAATACGTAATGACATTAAAGTTAACTTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCaa                                                                         <  1:371801/140‑1 (MQ=255)                                                                                          ttACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCg                                               >  2:668904/1‑140 (MQ=255)                                                                                            aCAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGt                                             <  1:753606/140‑1 (MQ=255)                                                                                            aCAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGt                                             >  2:753606/1‑140 (MQ=255)                                                                                             cAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGtc                                            >  2:605639/1‑140 (MQ=255)                                                                                                  aaCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGt                                       >  1:123375/1‑140 (MQ=255)                                                                                                  aaCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGt                                       >  1:808309/1‑140 (MQ=255)                                                                                                   aCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTc                                      >  1:439571/1‑140 (MQ=255)                                                                                                    ccTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTcc                                     <  2:643725/140‑1 (MQ=255)                                                                                                    ccTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTcc                                     <  2:808309/140‑1 (MQ=255)                                                                                                          gtCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTcc                                     <  2:464741/134‑1 (MQ=255)                                                                                                          gtCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTcc                                     >  1:464741/1‑134 (MQ=255)                                                                                                             gCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTGCGGATCAAGTTTGTCGGTCTCTGTCCCTTTATggg                            >  2:385326/1‑140 (MQ=255)                                                                                                                  ctctGGATTAACGGATAATAGGCGGC‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGttt                       >  2:711431/1‑140 (MQ=255)                                                                                                                                       gcggc‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGa  <  1:541240/140‑1 (MQ=255)                                                                                                                                       gcggc‑TTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGGCCGTTTAGGGGAGTGTCCAAAAAACTGGAAACCGCGa  >  1:396648/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        CTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGA  >  NC_000913/1705607‑1705881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑