Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,026	(C)6→5	coding (627/1179 nt)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,021	0	C	.	100.0%	118.2 / NA	29	coding (632/1179 nt)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/17);  total (12/17)

ACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAC  >  NC_000913/2637883‑2638147                                                                                                                                           |                                                                                                                               acGCCGTTAACAACGACTGGAGACGTGTCAATATCCCTCGGACGGTCACTTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGcc                                                                                                                               <  2:264020/140‑1 (MQ=255)      gTTAACAACGACGGGAGCCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTCGGAAATACGCTGCTGCCAAATCATCGGGCCCTGTTCCAGCAGGACTGCGCTTACGCTACCATTATCGcccccac                                                                                                                          >  2:346049/1‑138 (MQ=255)           caacGACGGGAGTCGTGTCAACCTCGCTCGGACGGTCGATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACgg                                                                                                                    <  1:324529/140‑1 (MQ=255)                 cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCAc                                                                                                              >  1:640290/1‑140 (MQ=255)                 cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGC‑GACGCGACCATTATCG‑CCCCCACGACGGCCGCAcc                                                                                                             >  1:611928/1‑140 (MQ=255)                    gAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCaa                                                                                                           >  2:603875/1‑140 (MQ=255)                      gTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGcc                                                                                                                  <  2:567988/131‑1 (MQ=255)                      gTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGcc                                                                                                                  >  1:567988/1‑131 (MQ=255)                              aaCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACTCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGTCGGCCGCACCAAAAGCCGTTGt                                                                                                 >  2:636596/1‑140 (MQ=255)                                   cGCTCAGACGGTCAACTTCGGTGGAACCGATCGCCTGAGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                            <  1:332952/140‑1 (MQ=255)                                            ggTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                   <  1:210588/140‑1 (MQ=255)                                               cAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCAc                                                                                <  2:429565/140‑1 (MQ=255)                                                aaTTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACg                                                                               <  2:611425/140‑1 (MQ=255)                                                  tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCa                                                                             <  1:636596/140‑1 (MQ=255)                                                  tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGC‑GACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCaa                                                                            <  2:611928/140‑1 (MQ=255)                                                      ggTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACACAAaga                                                                         >  1:112776/1‑140 (MQ=255)                                                                gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCGCCAAAAGCCGTTATCGACGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                               >  2:322102/1‑140 (MQ=255)                                                                gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGGTGTCGGCACAGTCTGGCCACGGAAAGAGAGCGAAGGc                                                               >  1:254054/1‑140 (MQ=255)                                                                   gCCTGGGAAATACGCTGCTGCCTAATCATCTTGCCCTGTCCCATCAGCCCTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                            <  2:254054/140‑1 (MQ=255)                                                                   gCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                            <  2:111106/140‑1 (MQ=255)                                                                   gCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                            <  2:763042/140‑1 (MQ=255)                                                                   gCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACACAAAGAGAGCGAAGGCata                                                            <  2:112776/140‑1 (MQ=255)                                                                                                           ccATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGt                    >  1:779518/1‑140 (MQ=255)                                                                                                             aTCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAACCGTTGTCGGCGCAGACTCGCCACGCAAAGTGCGCGAAGGCATATCAAGGATAACTGTCCATTTGACACCGCCGTCAGCTTCGTTc                  >  2:740094/1‑140 (MQ=255)                                                                                                                aGCACTGCGCTGACGCGACCATAATCG‑CCGCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgc               <  1:317465/140‑1 (MQ=255)                                                                                                                   aCTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATGTCGAGGTTCACTTTCCATTTGACAGCGCCGGCAGCTTCGTTCAGCGCt            >  2:317465/1‑140 (MQ=255)                                                                                                                   aCTGCGCTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCt            >  1:774298/1‑140 (MQ=255)                                                                                                                       cgcTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCtt           >  2:544456/1‑137 (MQ=255)                                                                                                                       cgcTGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCtt           <  1:544456/137‑1 (MQ=255)                                                                                                                         cTGACGCGACCATTACCG‑CCCCTACGACGTCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAAc      <  1:244541/140‑1 (MQ=255)                                                                                                                          tGACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACt     >  1:559097/1‑140 (MQ=255)                                                                                                                           gACGCGACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTg    <  2:774298/140‑1 (MQ=255)                                                                                                                             cgcgACCATTATCG‑CCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAc  <  2:775965/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                               ACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAC  >  NC_000913/2637883‑2638147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑