Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,323	(A)8→7	coding (20/483 nt)	yqeJ →	protein YqeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,316	0	A	.	100.0%	214.7 / NA	51	coding (13/483 nt)	yqeJ	protein YqeJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (23/28);  total (23/28)

AAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAG  >  NC_000913/2989177‑2989451                                                                                                                                            |                                                                                                                                        aaaCATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATtata                                                                                                                                         <  2:189577/140‑1 (MQ=255)       ccGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTATTTAACCCAGTCTTGCAGTGAGGTTAATCGTAATTGATTATaaaaaaa                                                                                                                                   >  2:399177/1‑140 (MQ=255)          tGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTCACCGTGAGGTTAATCGTGATTAATTATaaaaaaactt                                                                                                                                >  1:336784/1‑137 (MQ=255)               ataCTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTtattat                                                                                                                          <  1:835151/140‑1 (MQ=255)                 aCTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTAttattt                                                                                                                        >  1:580496/1‑140 (MQ=255)                    tCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATtatttatt                                                                                                                     >  2:181157/1‑140 (MQ=255)                     cccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTattatt                                                                                                                         >  2:740219/1‑135 (MQ=255)                     cccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTattatt                                                                                                                         <  1:740219/135‑1 (MQ=255)                     cccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTatttattt                                                                                                                    >  2:712211/1‑140 (MQ=255)                     cccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTatttattt                                                                                                                    >  1:785166/1‑140 (MQ=255)                     cccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTatttattt                                                                                                                    >  2:631461/1‑140 (MQ=255)                      ccTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGACTGACTA‑GAAAAAATCTTATTATTTAtttt                                                                                                                   >  1:399406/1‑140 (MQ=255)                       ctctTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTAACGTGAGGTGAATTGTGATTGATTAT‑AAAAATACTTATTATTTATTTTa                                                                                                                  >  2:274074/1‑140 (MQ=255)                          ttGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGtt                                                                                                               >  2:37979/1‑140 (MQ=255)                           tGCAAAATCCTCACCGTCGATGGTTGTTATGGCATGCAATAAAAACTAGAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGttt                                                                                                              <  2:399406/140‑1 (MQ=255)                           tGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGttt                                                                                                              >  1:544754/1‑140 (MQ=255)                                  tCCTCACCGGGGATGCTTGTTACGGTATGCAATAAAATCTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAg                                                                                                       <  1:399177/140‑1 (MQ=255)                                  tCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAg                                                                                                       <  1:712211/140‑1 (MQ=255)                                  tCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAg                                                                                                       <  2:580496/140‑1 (MQ=255)                                  tCCTCACCGGGGATGATTGTTACGGAATGTAATAAAATCTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAg                                                                                                       <  1:274074/140‑1 (MQ=255)                                      cACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCa                                                                                                        <  1:537071/135‑1 (MQ=255)                                      cACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCa                                                                                                        >  2:537071/1‑135 (MQ=255)                                       aCCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGt                                                                                                  >  1:88086/1‑140 (MQ=255)                                         cGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGttt                                                                                                <  2:88086/140‑1 (MQ=255)                                         cGGGGATGATTGTTATGCCATGCAATAAAAACTATGACCAACATGAAGCGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGttt                                                                                                <  2:272208/140‑1 (MQ=255)                                                gatTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTctct                                                                                         <  2:785166/140‑1 (MQ=255)                                                    gTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTACTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCAc                                                                                     <  2:544754/140‑1 (MQ=255)                                                     ttATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                                                                                    <  1:37979/140‑1 (MQ=255)                                                     ttATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                                                                                    >  2:758905/1‑140 (MQ=255)                                                      tATGGCATGCAATAAAAACTATTACCAACATGAAGTGGCAAACTGTTTTACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTg                                                                                   <  2:370826/140‑1 (MQ=255)                                                        tGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTc                                                                                 <  1:796991/140‑1 (MQ=255)                                                        tGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTc                                                                                 <  1:758905/140‑1 (MQ=255)                                                        tGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTc                                                                                 <  1:431652/140‑1 (MQ=255)                                                        tGGCATGCAATAAAAACTATAACCAACATCAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTc                                                                                 <  1:332897/140‑1 (MQ=255)                                                          gCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTa                                                                               >  1:128706/1‑140 (MQ=255)                                                             tGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTc                                                                            >  2:458059/1‑140 (MQ=255)                                                             tGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTc                                                                            >  2:56118/1‑140 (MQ=255)                                                                          ataACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTaca                                                                       >  1:734451/1‑132 (MQ=255)                                                                          ataACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTaca                                                                       <  2:734451/132‑1 (MQ=255)                                                                           taACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGaaa                                                              <  2:128706/140‑1 (MQ=255)                                                                           taACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGaaa                                                              <  1:181157/140‑1 (MQ=255)                                                                              ccAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGaaaaaa                                                           >  2:29089/1‑140 (MQ=255)                                                                                  cATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGat                                                       <  1:631461/140‑1 (MQ=255)                                                                                  cATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCCATACTTACAATGCAGAAAAAATGat                                                       >  1:330096/1‑140 (MQ=255)                                                                                   aTGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGata                                                      <  1:458059/140‑1 (MQ=255)                                                                                       aGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGAtatata                                                  <  1:56118/140‑1 (MQ=255)                                                                                                cTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACAt                                         <  1:29089/140‑1 (MQ=255)                                                                                                             tCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTg                            >  2:797994/1‑140 (MQ=255)                                                                                                             tCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTg                            >  1:852577/1‑140 (MQ=255)                                                                                                                tACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGt                         <  1:665069/140‑1 (MQ=255)                                                                                                                     tGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAAttt                    >  2:82459/1‑140 (MQ=255)                                                                                                                            aaTCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCTCTACAAATTAGGTAATTTTTAATGa             >  1:395310/1‑140 (MQ=255)                                                                                                                                 tgattgatTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTa                 <  1:94825/131‑1 (MQ=255)                                                                                                                                 tgattgatTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTa                 >  2:94825/1‑131 (MQ=255)                                                                                                                                       attAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAg  <  1:82459/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        AAACATCCGTGGTTATACTTCCCTCTTGCAAAATCCTCACCGGGGATGATTGTTATGGCATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAG  >  NC_000913/2989177‑2989451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑