Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,219,263	(T)5→4	coding (668/1737 nt)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,219,259	0	T	.	100.0%	153.2 / NA	37	coding (664/1737 nt)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/17);  total (20/17)

ATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGC  >  NC_000913/4219121‑4219394                                                                                                                                           |                                                                                                                                        attattCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtt                                                                                                                                        >  1:861261/1‑140 (MQ=255)   tattCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAtttt                                                                                                                                      >  1:489645/1‑140 (MQ=255)     ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgc                                                                                                                                    <  1:192147/140‑3 (MQ=255)     ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgc                                                                                                                                    >  2:192147/1‑138 (MQ=255)     ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgc                                                                                                                                    >  1:753331/1‑138 (MQ=255)     ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgc                                                                                                                                    >  1:6666/1‑138 (MQ=255)      tCGCCATCTGACGGAAACGCTGCGGACAGACCACCTCGCCGAAAGTCACTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgct                                                                                                                                   <  2:229776/140‑4 (MQ=255)       cGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCAttttgctg                                                                                                                                  >  1:492609/1‑136 (MQ=255)          cATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCGGGTAGGCAGACTGATCCCACCTTCCGGCACTTTGCCA‑TTTTGCTGCCg                                                                                                                              >  2:328042/1‑140 (MQ=255)           aTCTGACGGAAACGCTGTGGACTGACAACCTCGCGAAAACTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGa                                                                                                                             <  2:305535/140‑1 (MQ=255)                   gAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAg                                                                                                                     <  2:6666/140‑1 (MQ=255)                    aaaCGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAga                                                                                                                    <  1:491765/140‑1 (MQ=255)                       cGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAgacgg                                                                                                                 >  1:780083/1‑140 (MQ=255)                          tGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAgacggacg                                                                                                              >  2:450975/1‑140 (MQ=255)                            gggACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGgacgac                                                                                                            >  2:49120/1‑140 (MQ=255)                             ggACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGacgacg                                                                                                           <  2:861261/140‑1 (MQ=255)                              gacagacaACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACgg                                                                                                          >  2:652182/1‑140 (MQ=255)                                cagacaACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTGCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCg                                                                                                        <  2:816980/140‑1 (MQ=255)                                  gacaACCTCGCGGAAAGTCATTTACAGGTGGCGTACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAATCTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAg                                                                                                      <  2:547018/140‑1 (MQ=255)                                   acaACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGt                                                                                                     >  1:815303/1‑140 (MQ=255)                                      aCCTCGCGGAAAGTCATTTACATGTAGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                                                  <  1:406605/140‑1 (MQ=255)                                      aCCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                                                  <  2:492609/140‑1 (MQ=255)                                        cTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGGTCCTCCTGACGGATGACGGCGAGttattt                                                                                                >  1:411117/1‑140 (MQ=255)                                        cTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttattt                                                                                                >  2:627250/1‑140 (MQ=255)                                          cgcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGc                                                                                                         >  1:141457/1‑129 (MQ=255)                                          cgcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGc                                                                                                         <  2:141457/129‑1 (MQ=255)                                          cgcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                                                              <  2:815303/140‑1 (MQ=255)                                                aaGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATAc                                                                                        <  1:49120/140‑1 (MQ=255)                                                  gTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATAcct                                                                                      <  1:450975/140‑1 (MQ=255)                                                            ggTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACGCCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgac                                                                            <  1:544780/140‑1 (MQ=255)                                                             gTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgacg                                                                           >  2:514859/1‑140 (MQ=255)                                                              tGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgacga                                                                          <  1:627250/140‑1 (MQ=255)                                                               ggCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCTCCAGACGGACGATGGCGAGTTATTTCTTGATACCTGCCTgacgacgac                                                                         >  2:338739/1‑140 (MQ=255)                                                               ggCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgacgac                                                                         >  1:497278/1‑140 (MQ=255)                                                                      gaacTGTTTTACCGCAATAAAGTCGCCTGTCTGGTAGCCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGaa                                                                  <  1:338739/140‑1 (MQ=255)                                                                                      aTAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACGTGCCTGAGGACGACTGCCGAACCGAGCATTGTTTTTg                                                  >  1:374869/1‑140 (MQ=255)                                                                                        aaaGCCGCCTGGCTGGTAGGCATACTGATCAAACCGTCCAGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGc                                                <  2:374869/140‑1 (MQ=255)                                                                                           gccgccTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCa                                                           <  2:768902/126‑1 (MQ=255)                                                                                           gccgccTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCa                                                           >  1:768902/1‑126 (MQ=255)                                                                                               ccTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCtt                                              <  2:797016/135‑1 (MQ=255)                                                                                               ccTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCtt                                              >  1:797016/1‑135 (MQ=255)                                                                                                                     cacaCCTTCCGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATgc                   >  2:812101/1‑140 (MQ=255)                                                                                                                             ccGGCACATTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGc           >  1:520920/1‑140 (MQ=255)                                                                                                                                  acaTTGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCa      >  2:530564/1‑140 (MQ=255)                                                                                                                                     ttGCCA‑TTTTGCTGCCGATCCACCAAACGTACGACGGTGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAgcg   <  1:262572/140‑1 (MQ=255)                                                                                                                                      tGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTCTATCGTTTATGCGACGCTGCCCGCAgcgc  >  1:293104/1‑140 (MQ=255)                                                                                                                                      tGCCA‑TTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTAATTTATGGTATATGGGCCGCTGCCCGCAgcgc  >  2:262572/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        ATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGC  >  NC_000913/4219121‑4219394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑