| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,269,672 | (A)7→6 | coding (259/714 nt) | aphA → | acid phosphatase/phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,269,666 | 0 | A | . | 97.4% | 148.4 / ‑2.7 | 38 | coding (253/714 nt) | aphA | acid phosphatase/phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (17/20); minor base C (1/0); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTACGCCGCG > NC_000913/4269534‑4269801 | gCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGGTGACACGGGACTATATTACAGTCCGTGCTTAAGGCGCGGCaaaaaacc > 2:407506/1‑138 (MQ=255) cAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTGCCAGTCCTGGCGTCTGGCGC‑GCCAAAAAACCTTCTCg > 1:388041/1‑140 (MQ=255) cattcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAg < 2:798661/140‑1 (MQ=255) ttcattGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCCGACTTCTGGGGCGGC‑ACAAAACCGTCTCGCCAGAAAGCg > 1:351824/1‑140 (MQ=255) attGGGTTTCGGTCGCCAAAATTGAAAATAGGCACGCAGAGCGTCCGCGAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAg < 2:388041/140‑1 (MQ=255) gggTTTCGGTCGCACAAGTTGACAATAGCCTCGCACGGCATCCGCCAACGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGAtt < 2:276968/140‑1 (MQ=255) ggTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTa > 2:160990/1‑140 (MQ=255) gTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTAt < 2:17420/140‑1 (MQ=255) tttCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATc > 1:580824/1‑140 (MQ=255) tCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTg > 1:192035/1‑140 (MQ=255) ggTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa < 2:1190/140‑1 (MQ=255) cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa > 2:514554/1‑135 (MQ=255) cacaAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGaa < 1:514554/135‑1 (MQ=255) aTAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTggg < 1:160990/140‑1 (MQ=255) tAGCCTCGCAGGGCGTCTGCCACTGGCGGTGCGGTGTGATATCGATGATACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGa < 2:351824/140‑1 (MQ=255) aGCCTCGCAGAGCGTACGCCAAAGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGaa < 1:350493/140‑1 (MQ=255) ccTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAATATTATCTGGAAAAGCCTGTGTTCTGGGaaaa > 2:306756/1‑140 (MQ=255) gCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACaa < 2:192035/140‑1 (MQ=255) cGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCt > 1:131002/1‑140 (MQ=255) cAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg < 2:472172/140‑1 (MQ=255) cAATGGCGGTGGGGTATGATATCGATGACGCGGTACTGTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTggg < 1:407506/140‑1 (MQ=255) ggggTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAg < 2:131002/140‑1 (MQ=255) ttGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTc > 2:601341/1‑140 (MQ=255) tCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAag > 2:442338/1‑140 (MQ=255) cGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAtt > 2:855878/1‑125 (MQ=255) cGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAAtt < 1:855878/125‑1 (MQ=255) tGACACGGTACTTTTTTCCAGTCCGCGCTCCTGGCGCTGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGt < 1:331064/140‑1 (MQ=255) acacGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGtcg < 1:306756/140‑1 (MQ=255) tACTTTTTTCCAGTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGcc > 1:160740/1‑140 (MQ=255) aGTCCGGGCTTCTGGCGCGGC‑ACAAAACCTTCTCGCCAGTAAGCGAAGCTTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCtgattgat < 2:231613/140‑1 (MQ=255) gTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAACAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGata > 1:810390/1‑140 (MQ=255) gTCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTGAGCATTCGAAAGGAGGTCGCTAGCCAGCTGATTGata > 1:231613/1‑140 (MQ=255) tCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGata > 1:659512/1‑139 (MQ=255) tCCGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGata < 2:659512/139‑1 (MQ=255) ccGGGCTTCTGGCGCGGC‑AAAACACTTTCGCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATg < 1:326449/140‑1 (MQ=255) cGGGCTTCTGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGTATGAATTCAGCAGTGCTAAAGAGGTAGCTCAACAGCTGATTGATATGc > 2:391604/1‑140 (MQ=255) tGGCGCGGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTGAGCATTCCTAAAAAGGTCGCTCGCCCGCTGATTGATATGCATGTAcct > 2:326449/1‑138 (MQ=255) cgcgGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTAcgccgc > 2:652857/1‑140 (MQ=255) gcgGC‑AAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTACGCcgcg < 2:580824/140‑1 (MQ=255) | GCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAAGCGAAGATTATCTGAAAAATCCTGTGTTCTGGGAAAAAATGAACAATGGCTGGGATGAATTCAGCATTCCAAAAGAGGTCGCTCGCCAGCTGATTGATATGCATGTACGCCGCG > NC_000913/4269534‑4269801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |