| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,394,870 | (G)5→4 | coding (805/1548 nt) | nnr → | NAD(P)HX epimerase/NAD(P)HX dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,394,866 | 0 | G | . | 80.7% | 73.2 / 21.1 | 31 | coding (801/1548 nt) | nnr | NAD(P)HX epimerase/NAD(P)HX dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base . (8/17); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
TTGGCTGGCAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGA > NC_000913/4394728‑4395000 | ttGGCTGGCAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCAcggcgg > 2:530182/1‑140 (MQ=255) ctggcAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCggggct < 1:838321/140‑3 (MQ=255) tggcAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCggggcta > 2:65349/1‑137 (MQ=255) tggcAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCggggcta < 1:547174/140‑4 (MQ=255) gAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACgggggg > 2:139946/1‑139 (MQ=255) gTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCg < 1:530182/140‑1 (MQ=255) ttCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGGCATTCGTTTGACGGGGGGAGCGGCGCTGCgcg > 2:756879/1‑138 (MQ=255) ttCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCgtgc > 1:97627/1‑140 (MQ=255) ttCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCgtgc < 1:192905/140‑1 (MQ=255) cagAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCgtgctggtg < 1:139946/140‑1 (MQ=255) cagAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCgtgctggtg < 1:38648/140‑1 (MQ=255) cagAACAACTTTCTCACAGGCTCAAACCGCGTCGCCCGACTTCGCATACAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCgtgctggtg < 2:309973/140‑1 (MQ=255) caacTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCAATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTgctggtgctggt < 1:713042/140‑1 (MQ=255) gacagctcACTGGCTCAAACCGTGTAGCCAGACTTCGTATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGttt < 1:222381/135‑1 (MQ=255) ttCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGTTGGTGCTGGTTTAGt < 1:692036/140‑1 (MQ=255) tcACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGTG‑TATTCGTGTTGCGGGGGGGGCGGCGGTGCGTGGTGGTGGCGGGTTTGTCCg > 2:494832/1‑140 (MQ=255) tcACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGG‑TATTCGTTTGACGCGGGGAGGAGCGGCGAGGGGCGGGGCTGGTTTTGTccc > 1:424182/1‑139 (MQ=255) aaaCCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGG‑TATTCGTATGACTGGGGGAGGGGCGGTGCGGGCTGGGGGTGGGTTTGTCCGGGTAcctgtt > 2:222381/1‑135 (MQ=255) aCCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCg < 2:97627/140‑1 (MQ=255) aCCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCg < 1:65349/140‑1 (MQ=255) ccGCGTCGACCGACTTTGCATACAGGCGATGGCTGCCGGGTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGc < 2:424182/140‑1 (MQ=255) cgTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTTTTCCTATGGCGGGGGAAGCGGCGCGGGGGGCGGGGGTTGGTTTAGCCCGCGAACTGCCCCGCAgg > 2:86124/1‑139 (MQ=255) cgTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCCATTCGTATGACGGGGGGGGCGGCGCCCCGGACTGAGGCTGGGTGTGTCGCAGGACTGACCCGccgg > 2:222340/1‑137 (MQ=255) tCGCATAATGGCGATCCCGGGCCGCTGGTAAGTGTTGGTTGCGATCACCGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTgcg < 2:423962/140‑1 (MQ=255) cGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGG‑TGTTCGTGTGACGGGGGGAGCGGCGCTGCGCGCTGGTGCTGGGTTATTCCGAGTAGTGGCCCGCAGGGAAAAAATTacgc > 2:442190/1‑140 (MQ=255) ggCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGGAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGctgctg > 1:33435/1‑140 (MQ=255) cggcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGAcc < 1:756879/140‑1 (MQ=255) aaTTATCGGTGGCGATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGGGCTGGGTTTGTCCCCGAACTGACCCCCCGGGAAAACATTGCGCCGCTGCTGACTTGACGGCCGGGAATGAt > 2:122046/1‑140 (MQ=255) aTCGGTGGCGATCACGGCACGGCGGGGCCCA‑TCGTATGACGGGGGGAGGCGCGGGTCGGGCTGGTGCTGGTTTTGTCCGAGGAATTCACCCCAGCGAAAACAATCGCCCGCTCGTGACTGCACGACCGGAAATGAAgggg > 2:19725/1‑138 (MQ=255) tCGGTGGCGATCACGGCACGGC‑GGGGCTATTTGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGc > 1:152604/1‑140 (MQ=255) gTGGCGATCACGGCACGGC‑GGGGATATTTGTATGTAGGGGGGAGCGGCGCGGCGGGCTGGGGCTGGTTTAGTCCGAGGAATCACCCCCCGTGGAAACAATGGGCCGCTGCTGCCTGCCCGACCGGAAGTGATGGTGCATg > 2:756258/1‑140 (MQ=255) gATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTg < 1:19725/140‑1 (MQ=255) gATCACGGCACGGC‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTg < 1:86124/140‑1 (MQ=255) ggcacggc‑GGGGCTAATCGTATGACAGGGTAAGCGGCTCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATg < 1:311600/140‑1 (MQ=255) gcacggc‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATgg < 2:727245/140‑1 (MQ=255) cacggc‑GGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGa < 1:442190/140‑1 (MQ=255) | TTGGCTGGCAGGTCAGGAGACGAAAATTCAGCGGTTTTCAGCAGAACAACTTTCTCACTGGCTAAAACCGCGTCGCCCGACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGA > NC_000913/4394728‑4395000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |