| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 742,314 | (C)5→4 | coding (243/1482 nt) | dtpD ← | dipeptide:H(+) symporter DtpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 742,310 | 0 | C | . | 98.2% | 216.7 / ‑0.5 | 56 | coding (247/1482 nt) | dtpD | dipeptide:H(+) symporter DtpD |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base . (31/23); minor base T (0/1); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATT > NC_000913/742176‑742445 | cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCGAGCACCAGATGACCTATCGCCATCAACAACGccccag > 1:281868/1‑138 (MQ=255)cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccccag > 2:852398/1‑138 (MQ=255)cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccccag > 1:113849/1‑138 (MQ=255)cTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCAAGCACCACATGTCCCATCGCCGTCAACAACGccccag > 1:257865/1‑138 (MQ=255) tCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccccagc > 1:625351/1‑137 (MQ=255) cGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCGCTGGCACCGAGCGCCACATGACCGACCGCCCTCAACAACGccccagca > 2:330449/1‑136 (MQ=255) cTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGa < 2:625351/140‑1 (MQ=255) gTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCTCCCAGCACCACATGACCGATCGCCATCAACAAC‑CCCCCAGCATCACCGCCATGCGATTGcc < 2:21634/140‑1 (MQ=255) aGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCgag > 2:125986/1‑140 (MQ=255) aTTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATGAACGACG‑GCCCAGCATGACCGACATGCGATTGCCGAGaa > 2:233234/1‑140 (MQ=255) cAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATc < 2:69107/140‑1 (MQ=255) ggCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCg > 2:23989/1‑140 (MQ=255) gcgaTAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTGACTGGCACCCAGGACCACATGACCGATCGCGATCAACAACG‑GCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGc > 2:493352/4‑140 (MQ=255) gCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGc > 2:155357/1‑140 (MQ=255) ccATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGGTGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGcc > 2:556781/1‑140 (MQ=255) aGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCaaaa > 1:788893/1‑140 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCa > 1:12937/1‑140 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCa > 1:847912/1‑140 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCa > 1:609303/1‑140 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCa > 2:95325/1‑140 (MQ=255) aaTCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGAt < 2:113849/140‑1 (MQ=255) ccAGGGACGGATAGAGGCATGACGGATGGATCTCAGTGGACCCCAGCAACACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg < 2:393867/140‑1 (MQ=255) ccAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg > 2:593180/1‑140 (MQ=255) ccAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg > 2:124695/1‑140 (MQ=255) ccAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg < 1:125986/140‑1 (MQ=255) ccAGGGACAGATAGAGCAATGACGGATGGATCTCCCTGGCACCCAGCACCACATGACCGATCGCCAACAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGACCTTTATCCGCCAAAAAGCCACCGAGGATTGGCg < 2:29344/140‑1 (MQ=255) gACAGATAGAGGAATCACGGATAGAACTCACTGGCACACAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACa < 1:330449/140‑1 (MQ=255) agagGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCa < 2:12937/140‑1 (MQ=255) agGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGc > 1:33261/1‑140 (MQ=255) ggAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCg > 2:828413/1‑140 (MQ=255) gAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGa > 2:602378/1‑140 (MQ=255) gAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGa > 2:530478/1‑140 (MQ=255) aTGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAAc > 1:861139/1‑140 (MQ=255) gACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAATGCCACCGTGGAATGACGTGACATACACCAGCGAACAg > 1:416000/1‑140 (MQ=255) aCGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGt < 1:124695/140‑1 (MQ=255) aCGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGt < 1:816745/140‑1 (MQ=255) gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAgg > 1:666358/1‑140 (MQ=255) tggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCATCGAGGATTGGCGTGACATACACCAGCGAACAGTAGgcg > 1:562577/1‑140 (MQ=255) tggatCTCACTGGCACCAAGCCTCACATGACTGATAGCCATCAACAAC‑TCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGgcg < 2:257865/140‑1 (MQ=255) cACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCTCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATa < 1:47624/140‑1 (MQ=255) cACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATa > 1:32814/1‑140 (MQ=255) ggCACCCAGCACCACTTGACCGATCTCCAACAACAAC‑CCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTc < 1:233234/140‑1 (MQ=255) gCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTaaa > 2:529451/1‑133 (MQ=255) gCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTaaa < 1:529451/133‑1 (MQ=255) gCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg < 1:95325/140‑1 (MQ=255) cACCCAGCACCACATGACCGATCGCCAACAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCATGACATATACCAGCGACCAGTCGGCGCTAAATAACTCGt > 2:262454/1‑140 (MQ=255) aCCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTa < 1:530478/140‑1 (MQ=255) aGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCg > 2:639776/1‑140 (MQ=255) gCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt < 1:852398/140‑1 (MQ=255) caccacATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGTCGTGACATCCACAAGCAAACAGTAGGCGCTTAATAACTCGTAGGCGTg > 2:279427/1‑140 (MQ=255) ccacATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg < 1:556781/140‑1 (MQ=255) acaTGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa < 1:155357/140‑1 (MQ=255) tGACCGATCGCCCTCAACACCG‑CCCCAGCACCGCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 2:847912/140‑1 (MQ=255) tGACCGATCGCCATCTACAACG‑TCCTAGCTTCACCGCTATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 1:279427/140‑1 (MQ=255) tGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 2:33261/140‑1 (MQ=255) tGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 2:562577/140‑1 (MQ=255) gACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt < 1:23989/140‑1 (MQ=255) gACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt < 1:516537/140‑1 (MQ=255) ccGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTg > 2:639643/1‑140 (MQ=255) gtCGCCGCCAGCAACGCCCCCA‑CCCCCCCCCCATGCGATTCCCGCGAAGTTCATCCGCAACGAATCCCCCGCGGTCTGGCGTTCCATCCCCCAGCGAACATTATGGGCTAAATAACTCGTAGGCGTGCGTATCGttctct < 2:493969/139‑5 (MQ=255) aTCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAt > 1:74019/1‑140 (MQ=255) cGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAttt < 2:666358/140‑1 (MQ=255) ccATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa < 1:595149/140‑1 (MQ=255) cATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAg > 2:145022/1‑140 (MQ=255) aTCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGt < 1:639643/140‑1 (MQ=255) acaacG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt > 2:461270/1‑140 (MQ=255) caacG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAtt > 2:841976/1‑140 (MQ=255) | CTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATT > NC_000913/742176‑742445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |