Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,269,425	(C)5→4	intergenic (+36/+278)	rdlA → / ← ldrB	putative antisense RNA regulator of LdrA/small toxic polypeptide LdrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,269,421	0	C	.	100.0%	117.0 / NA	40	intergenic (+32/+282)	rdlA/ldrB	putative antisense RNA regulator of LdrA/small toxic polypeptide LdrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/19);  total (21/19)

CCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCAT  >  NC_000913/1269283‑1269553                                                                                                                                           |                                                                                                                                     ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGcc                                                                                                                                     >  2:57179/1‑140 (MQ=255)     gttgtAATGACAACATTTTGCGGCTATTCTTGAATTGTTGTGGTTCAAGATTAGCCACCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGccccgc                                                                                                                                 <  2:373118/140‑3 (MQ=255)         tAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACa                                                                                                                            <  1:572385/140‑1 (MQ=255)              acaacaTTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTgcg                                                                                                                       >  2:70216/1‑140 (MQ=255)                       tGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCgg                                                                                                              >  2:718321/1‑140 (MQ=255)                       tGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCgg                                                                                                              >  1:673022/1‑140 (MQ=255)                            cTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAAc                                                                                                         >  1:572355/1‑140 (MQ=255)                               ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGcc                                                                                                      >  2:849761/1‑140 (MQ=255)                                  ttGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGc                                                                                                   <  2:28796/140‑1 (MQ=255)                                       ttGTTCTGGTTCAAGGTTAGCCCCCGTTCGGTTGTCAAGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgt                                                                                              <  1:326366/140‑1 (MQ=11)                                       ttGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgt                                                                                              <  1:849761/140‑1 (MQ=255)                                       ttGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgt                                                                                              <  1:57179/140‑1 (MQ=255)                                         gTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgtgt                                                                                            >  2:130675/1‑140 (MQ=255)                                          ttCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg                                                                                           >  1:556250/1‑140 (MQ=255)                                          ttCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg                                                                                           >  2:679927/1‑140 (MQ=37)                                          ttCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg                                                                                           >  2:866081/1‑140 (MQ=255)                                                    aGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGGGCCTCACAGGATAACGGCGGCATGGTGTGGATACTACGa                                                                                 >  2:302646/1‑140 (MQ=11)                                                           cccccGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGa                                                                                         >  2:541573/1‑125 (MQ=17)                                                           cccccGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGa                                                                                         <  1:541573/125‑1 (MQ=11)                                                               cGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATc                                                                      <  2:572355/140‑1 (MQ=255)                                                               cGTTCTGTTGTCAGGCTGTACCTCTGAACGTGCGGGGCTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATc                                                                      <  1:394726/140‑1 (MQ=11)                                                                 ttCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATctt                                                                    <  1:130675/140‑1 (MQ=25)                                                                      ttgtCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcactt                                                               >  1:724254/1‑140 (MQ=18)                                                                             gTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCt                                                        >  1:117865/1‑140 (MQ=18)                                                                              ttGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTg                                                       >  2:690838/1‑140 (MQ=18)                                                                               tGTACCTCTCAACGTGCAGGGGGTTTCTCTTTCCAACAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGa                                                      <  2:391807/140‑1 (MQ=11)                                                                                gTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGaa                                                     >  2:117103/1‑140 (MQ=14)                                                                                gTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACATCATCCCTGaa                                                     >  2:174351/1‑140 (MQ=9)                                                                                    ctctCAACGTGCGGGGGTTTTCGCTCTCCGGCAACCAGTGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt                                                 <  1:302646/140‑1 (MQ=11)                                                                                      ctcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGttt                                               >  1:572524/1‑140 (MQ=11)                                                                                       tcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                              >  2:91849/1‑140 (MQ=11)                                                                                       tcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                              >  1:430789/1‑140 (MQ=11)                                                                                       tcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGCGTGGAAACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGCTTg                                              >  2:684279/1‑140 (MQ=11)                                                                                         aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                            <  2:724254/140‑1 (MQ=17)                                                                                             tGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                        <  2:673022/140‑1 (MQ=11)                                                                                              gCGGGGGTTTTCTCTTTCCAGCGACCAATGGCACCGGGGTTAAAG‑CCCAGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                       <  2:416092/140‑1 (MQ=11)                                                                                               cGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag                                      >  2:146096/1‑140 (MQ=11)                                                                                                           ctTTCCAGCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCGCACCGGATACCGTCGGCTTGGTGTGGATACTACGACTCAATTCATGTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCgg                          <  2:13169/140‑1 (MQ=11)                                                                                                                  gCAACCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCa                   <  1:70216/140‑1 (MQ=255)                                                                                                                    aaCCAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAAc                 <  1:91849/140‑1 (MQ=255)                                                                                                                      ccAATGCCACCAGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACATCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAg               <  1:174351/140‑1 (MQ=255)                                                                                                                            ccaccaGGGATAAAG‑TCCCGCAGCACTGCTCCTCACCTGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTa         <  1:402447/140‑1 (MQ=11)                                                                                                                                caGGGATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGt     <  1:866081/140‑1 (MQ=255)                                                                                                                                   ggATAAAG‑CCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCAt  <  2:572524/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     CCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCAT  >  NC_000913/1269283‑1269553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑