Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,417,546	(T)8→7	coding (218/276 nt)	racC ←	Rac prophage; protein RacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,417,539	0	T	.	100.0%	144.8 / NA	35	coding (225/276 nt)	racC	Rac prophage; protein RacC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/18);  total (17/18)

TTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTT  >  NC_000913/1417409‑1417666                                                                                                                                   |                                                                                                                                ttAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCtttttttctg                                                                                                                        >  2:510438/1‑137 (MQ=255)         cGTTTTGTTCAGTGATTGATCTTGCCGGAAACACACTTTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGa                                                                                                               <  1:397934/140‑1 (MQ=255)               gTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCttt                                                                                                         <  2:190937/140‑1 (MQ=255)                      gAATGATCTTGCCGGCTACACACTGTTCATAGCCTGCTCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGatat                                                                                                  <  1:417924/140‑1 (MQ=255)                       aaTGATCTTGCCGGAAACACACAGTTCATTGCCTGCGTCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                                                                 <  1:333223/140‑1 (MQ=255)                                 ccGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGcac                                                                                       >  1:515797/1‑140 (MQ=255)                                               ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTg                                                                              <  2:760984/135‑1 (MQ=255)                                               ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTg                                                                              >  1:760984/1‑135 (MQ=255)                                               ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACAcc                                                                         >  1:149379/1‑140 (MQ=255)                                                         gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcatc                                                                   >  1:634821/1‑136 (MQ=255)                                                         gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcatc                                                                   <  2:634821/136‑1 (MQ=255)                                                         gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAgt                                                               >  2:93299/1‑140 (MQ=255)                                                               tACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGatttt                                                         >  2:821652/1‑140 (MQ=255)                                                                  gCAGGCTATTTCTTTAAGATTTGACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACg                                                      <  1:377463/140‑1 (MQ=255)                                                                      gCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGc                                                  >  1:431280/1‑140 (MQ=255)                                                                       cTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGcc                                                 >  2:55813/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc                                        >  1:449410/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc                                        >  1:555977/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc                                        >  1:593670/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc                                        >  1:741013/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAcc                                        >  2:455858/1‑140 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAc                                         <  1:145527/139‑1 (MQ=255)                                                                                tcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGAc                                         >  2:145527/1‑139 (MQ=255)                                                                                 cAGATTTCACCTTTTTATTTCAGTGTAATTAGAGTTGTCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCa                                       <  1:282760/140‑1 (MQ=255)                                                                                  aGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCAt                                      <  1:529040/140‑1 (MQ=255)                                                                                    aTTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCg                                    <  2:515797/140‑1 (MQ=255)                                                                                        cACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGatat                                >  2:40346/1‑140 (MQ=255)                                                                                         aCCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATc                               <  2:741013/140‑1 (MQ=255)                                                                                              ttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACca                          <  1:40346/140‑1 (MQ=255)                                                                                               tAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACGCCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACcac                         <  1:510438/140‑1 (MQ=255)                                                                                                aaTTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGa                                          >  2:686887/1‑122 (MQ=255)                                                                                                aaTTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGa                                          <  1:686887/122‑1 (MQ=255)                                                                                                        tGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGaa                   >  2:491517/1‑137 (MQ=255)                                                                                                        tGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGaa                   <  1:491517/137‑1 (MQ=255)                                                                                                            aTTAGAGTTGCCAGACATTCGG‑ATTATTTGCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGtt            <  1:403655/140‑1 (MQ=255)                                                                                                                      ccAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAtttt  <  2:431280/140‑1 (MQ=255)                                                                                                                      ccAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAtttt  <  1:55813/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                TTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTT  >  NC_000913/1417409‑1417666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑