| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,892,882 | (C)6→7 | coding (354/1911 nt) | yoaA ← | putative 5' to 3' DNA helicase implicated in DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,892,876 | 1 | . | C | 97.1% | 109.0 / ‑3.4 | 35 | coding (360/1911 nt) | yoaA | putative 5' to 3' DNA helicase implicated in DNA repair |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (16/18); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG‑‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAG > NC_000913/1892753‑1893001 | gACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGcc < 1:841901/140‑1 (MQ=255) gCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTc > 1:705505/1‑140 (MQ=255) gCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTc > 1:629296/1‑140 (MQ=255) tgtgAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGtt < 2:617411/140‑1 (MQ=255) gtgAATCTTCCGCCACGCTGACGCAGGTGCTGATATCCCCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTc < 1:144416/140‑1 (MQ=255) aTCTTCCGCCACGCTGACGCAGGTGCTGATTTCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAgg < 2:629296/140‑1 (MQ=255) aTCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAgg < 2:705505/140‑1 (MQ=255) cTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCa < 2:663103/140‑1 (MQ=255) cGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAgg < 1:6367/140‑1 (MQ=255) aCGCAGGTGCTGATATCACCATCGACTGTTTGATTAGGCCAGGAGCGCAGCAGGATCAAATCGCTTAAGATTTGTCCCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAgc < 1:87653/140‑1 (MQ=255) gTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCtt > 2:827266/1‑140 (MQ=255) gTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCtt > 1:142777/1‑140 (MQ=255) aCCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGcc > 2:573860/1‑140 (MQ=255) ccATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTTCCGGCAGATCG‑‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCAc < 2:519212/140‑1 (MQ=255) aCTGTTTGATTAGACAAGGAGCCCATCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGc < 1:351177/140‑1 (MQ=255) cTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGcc > 2:665997/1‑140 (MQ=255) tGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCATCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGccc > 2:58349/1‑140 (MQ=255) ttGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGACCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGta < 1:15704/140‑1 (MQ=255) aTTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTatat < 1:78920/140‑1 (MQ=255) gACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCa < 1:665997/140‑1 (MQ=255) ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCATCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt < 1:58349/140‑1 (MQ=255) ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAAt < 1:573860/140‑1 (MQ=255) cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGTAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag > 2:560388/1‑140 (MQ=255) cagcagGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgag > 2:860995/1‑140 (MQ=255) cagGATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGGCCTTTCAGAAGCGCCACGTTGCCCGGATATTTCAATGCCTTTGAGACt > 2:301388/1‑140 (MQ=255) gATCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGGGCCTCGTGGCCCGTATGTTTCTATGCCTTTGAGACTGtt > 2:283879/1‑140 (MQ=255) tCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTgg > 1:603564/1‑140 (MQ=255) tCACATCGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTCCAGCAGCGTCACGTTGACCGTATATTTCAAGGCCTTTGAGACTGTTgg > 2:262537/1‑140 (MQ=255) cGCTTAAGATTTGTACCGGCAGGTCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgaga < 2:166566/127‑1 (MQ=255) cGCTTAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTgaga > 1:166566/1‑127 (MQ=255) gCTTAAGATTTGTACCGGCAGAACGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcg < 1:283879/140‑1 (MQ=255) cTTAAGATTTGTACCGGCAGATCGCCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATcg > 2:25595/1‑140 (MQ=255) tAAGATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCgg < 1:301388/140‑1 (MQ=255) gATTTGTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTg < 1:827266/140‑1 (MQ=255) gTACCGGCAGATCGC‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGACCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTagag > 2:730871/1‑140 (MQ=255) | GACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG‑‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAG > NC_000913/1892753‑1893001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |