Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,359	(T)5→6	coding (1052/1053 nt)	ccmH ←	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,354	1	.	T	100.0%	84.0 / NA	25	intergenic (+207/+4)	narP/ccmH	DNA‑binding transcriptional dual regulator NarP/holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (13/12);  total (13/12)

CTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTC‑TTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAA  >  NC_000913/2291216‑2291486                                                                                                                                            |                                                                                                                                     cTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCctc‑t                                                                                                                                     >  2:199867/1‑140 (MQ=255)      cAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTC‑tttttt                                                                                                                                <  1:466162/140‑2 (MQ=255)              cGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTC‑tttt                                                                                                                                  >  2:436412/1‑130 (MQ=255)              cGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTC‑tttt                                                                                                                                  <  1:436412/130‑1 (MQ=255)                     cgatcAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTgcg                                                                                                                  <  2:560392/140‑1 (MQ=255)                                 tGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGtt                                                                                                      >  1:784503/1‑140 (MQ=255)                                       tACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCg                                                                                                >  1:194564/1‑140 (MQ=255)                                           caATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTg                                                                                            <  1:208078/140‑1 (MQ=255)                                               tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGcgcg                                                                                        <  2:7982/140‑1 (MQ=255)                                                ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGa                                                                                       <  1:536561/140‑1 (MQ=255)                                                  gTAGGCCGGATAAGGCGTTCACGCCGCATTCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAta                                                                                     <  1:307981/140‑1 (MQ=255)                                                     gacaggATAAGGCGTTCCCGCCTCATCCGGCATAATCTACGCGCACTTGGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtacta                                                                                  <  2:364768/136‑1 (MQ=255)                                                          gATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtac                                                                                    >  1:787279/1‑133 (MQ=255)                                                          gATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtac                                                                                    <  2:787279/133‑1 (MQ=255)                                                              aGGCGTTCACGCCGCTTCCGGCGTAGACTAAGCGCACGTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATc                                                                         <  1:297861/140‑1 (MQ=255)                                                                    tCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtac                                                                                    <  2:199551/123‑1 (MQ=255)                                                                    tCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGAtac                                                                                    >  1:199551/1‑123 (MQ=255)                                                                     cACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCAc                                                                  <  1:199867/140‑1 (MQ=255)                                                                     cACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGGTGCATCGCCTGAGCGATACTACGTTCAATCAcctgtc                                                                  >  1:364768/1‑136 (MQ=39)                                                                      aCGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACg                                                                 <  2:760886/140‑1 (MQ=255)                                                                          cgcATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGAAAAATGTTGGATCCCCTGAGCGATACGACGTGAAATCACCACACGGCGa                                                             >  2:415937/1‑140 (MQ=255)                                                                                             gcgcACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAaga                                          >  2:147546/1‑140 (MQ=255)                                                                                                             gTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCAtctc                          <  1:147546/140‑1 (MQ=255)                                                                                                             gTCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCAtctc                          >  1:51666/1‑140 (MQ=255)                                                                                                              tCTAGCCCGATGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTcc                         >  1:178776/1‑140 (MQ=255)                                                                                                                        tGGCATCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCaa               >  2:178456/1‑140 (MQ=255)                                                                                                                             tCACCATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCt          >  2:57899/1‑140 (MQ=255)                                                                                                                             atgccATCGGGCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCt          >  2:661706/4‑140 (MQ=255)                                                                                                                                     gggCCTCTTTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCaaa  >  2:588051/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                     CTTATCAGGCCTACGTGGATCGATCAATTTATTGAATTTACACAATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACTAAGCGCACTTTGTCAACAGTCTAGCCCGATGGCATCACCATCGGGCCTC‑TTTTTATTTACTCTCCTGCGGCGACAAATGTTGCATCGCCTGCGCGATACTACGTTCAATCACCGCACGGCGAGTATCGTTGGCAGGTAAGAGTTTCAACATCATCTCCCACGCGGCAACGGCTTCGCCAAA  >  NC_000913/2291216‑2291486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑