| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,442,660 | (C)5→6 | coding (897/2007 nt) | mnmC → | fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,442,655 | 1 | . | C | 95.8% | 78.1 / ‑3.1 | 24 | coding (892/2007 nt) | mnmC | fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (11/12); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGG‑CCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCG > NC_000913/2442522‑2442787 | cgcgCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGGTTGCCAACGCGGTGTTGTCGCCGGCGCTATTACCGCGCGGGCGGGAGGGTAAC‑CTTTTTTTGGCGGGGGAGG‑cccccc > 2:166975/1‑139 (MQ=255) ggTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTggg < 1:762885/140‑1 (MQ=255) cACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGG‑CCCCCGCACTGGGTGCTTCCGGCa < 1:506667/140‑1 (MQ=255) agcaAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAgg < 2:541762/140‑1 (MQ=255) caAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGTAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAgggg < 1:166975/140‑1 (MQ=255) aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTTGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAggggg < 1:312345/140‑1 (MQ=255) aCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGgcg < 1:436179/140‑1 (MQ=255) aCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGgcg < 1:861964/140‑1 (MQ=255) gggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCt < 1:30723/140‑1 (MQ=255) gggAAGCGGCGATTATCAGCGGTGGTATTGCTAGCCCGTTGTTGTCGCAGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCt < 2:312325/140‑1 (MQ=255) cgATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGtt < 1:16827/140‑1 (MQ=255) aGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGgcgc > 2:52047/1‑140 (MQ=255) cgcgTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCCGGTGACGCTTTTTTGGGCGGATGGGGCCCCCCGGACTGGGGTCCTCCGGCAATCTCCAGGGGGCTCTGTATCCGTTATTAACCAAACACGTTGACCCGCTa > 2:112017/1‑140 (MQ=255) cgcgTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTa > 1:497885/1‑140 (MQ=255) tgtCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtt < 1:617924/140‑1 (MQ=255) ggCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCTCTAGACCGCTTTTTCTCTAATGCGTTTAc > 1:413954/1‑140 (MQ=255) gcgc‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACt > 1:91892/1‑140 (MQ=255) cgc‑gGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACtt > 1:683665/1‑140 (MQ=255) c‑gGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACtttt > 2:841568/1‑140 (MQ=255) c‑gGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACtttt > 2:21855/1‑140 (MQ=255) gCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGc > 2:593050/1‑140 (MQ=255) gCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGc > 1:572661/1‑140 (MQ=255) aTTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCtcg > 1:813117/1‑125 (MQ=255) aTTGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTCCTCTAATGCGTTTACTTTTGCtcg < 2:813117/125‑1 (MQ=255) tGCGCGGATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAAt > 2:811886/1‑140 (MQ=255) gATGAGGCCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCg < 1:811886/140‑1 (MQ=255) | CGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGC‑GGCTGGCAGGTAACGCTTTATTGCGCGGATGAGG‑CCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCG > NC_000913/2442522‑2442787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |