| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,602,375 | +G | coding (558/2019 nt) | hyfB → | hydrogenase 4 component B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,602,371 | 1 | . | G | 97.4% | 132.5 / ‑3.5 | 39 | coding (554/2019 nt) | hyfB | hydrogenase 4 component B |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (22/16); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGAT‑GG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAG > NC_000913/2602232‑2602503 | gTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGAt < 2:748318/140‑1 (MQ=255) tgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCgg > 2:542445/1‑140 (MQ=255) tgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCgg < 1:49866/140‑1 (MQ=255) tgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGAT‑g > 1:712535/1‑125 (MQ=255) tgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGAT‑g < 2:712535/125‑1 (MQ=255) cTGTCTTCCTGGTTTGTGGTCATTGTCAGCCAGGATACAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGcc < 1:420331/140‑1 (MQ=255) tGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCt > 2:183156/1‑140 (MQ=255) tCCTGGTTTCTGGTCATGGCCAGGCAGGATACAACTTCGGTCAACGCTCGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGAtt < 2:342943/140‑1 (MQ=255) cTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGAtttt > 1:467241/1‑140 (MQ=255) gTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTcc > 1:5472/1‑140 (MQ=255) tCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCg < 2:5472/140‑1 (MQ=255) tCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCg < 2:467241/140‑1 (MQ=255) cATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGc < 1:542445/140‑1 (MQ=255) tGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCAc < 2:612541/139‑1 (MQ=255) tGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCAc > 1:612541/1‑139 (MQ=255) ccAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCt < 1:183156/140‑1 (MQ=255) ggcaggATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTc > 2:490958/1‑140 (MQ=255) ggATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCt < 1:92656/133‑1 (MQ=255) ggATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCt > 2:92656/1‑133 (MQ=255) gATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACttt > 2:19170/1‑140 (MQ=255) gATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACttt > 1:508462/1‑140 (MQ=255) aaaaCGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTtctc < 1:490958/140‑1 (MQ=255) cGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCt > 2:819414/1‑140 (MQ=255) tCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGc > 1:639138/1‑140 (MQ=255) gCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGgt > 1:811305/1‑140 (MQ=255) cATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCTCACGCTTGCACATTGTCCGGGACTGGCGTCGGCGgtg > 1:258569/1‑140 (MQ=255) ctctACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTcc > 1:63711/1‑140 (MQ=255) cTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCctgctg > 1:57730/1‑140 (MQ=255) ttttATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGcc < 2:63711/140‑1 (MQ=255) aTCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGcgcg < 2:508462/140‑1 (MQ=255) aTCGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGcgcg < 2:57730/140‑1 (MQ=255) cGGTGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGaa < 1:19170/140‑1 (MQ=255) tGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGTTGTTCCTGCTGGCCGTTTTCGATTTTGGCGCGAAAGc > 1:263997/1‑140 (MQ=255) tGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAg > 1:69304/1‑139 (MQ=255) tGCTGATAATGATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAg < 2:69304/139‑1 (MQ=255) aTGATCGCCTTCTTGCTCAT‑GGCGGCGCGAACGCGGCAGCCTCGATTTCGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGgatga < 2:263997/140‑1 (MQ=255) gATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGgatga < 2:133185/138‑1 (MQ=255) gATCGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGgatga > 1:133185/1‑138 (MQ=255) cGCCTTCTTGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCg > 2:866225/1‑140 (MQ=255) cGCCTTCTTGCTGATCGG‑GGCGTGACAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCg < 1:411230/140‑1 (MQ=255) cttcttGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTACTGCTGGCCTTTTTCGTGTTTGGCGGGAAAACCGGGATGTTGCCGttt > 2:411230/1‑139 (MQ=255) cttGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTATTCGGTTTTGGCGCGATAGCCACGATGATGGCGTTGcac > 2:404742/1‑140 (MQ=255) tGCTGATGGG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCTGTTTTGGGGCGAAAGCCGGGATGATGCCGTTGCACAg > 1:292720/1‑140 (MQ=255) | GTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGAT‑GG‑GGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAG > NC_000913/2602232‑2602503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |