| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 285,647 | (G)6→7 | coding (253/1383 nt) | yagG → | putative D‑xylonate transporter YagG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 285,641 | 1 | . | G | 97.8% | 155.1 / ‑3.4 | 45 | coding (247/1383 nt) | yagG | putative D‑xylonate transporter YagG |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (12/32); total (13/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTG > NC_000913/285504‑285772 | tCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gg < 2:509734/140‑1 (MQ=255)tCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gg < 1:21850/140‑1 (MQ=255) ttCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTGTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gg‑gg < 2:327681/140‑1 (MQ=255) tCGGCCTGTCGGCGGCGATTATGGGGACGGTGTTTTTCGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑gg‑ggg < 1:314556/140‑1 (MQ=255) gggATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCg > 1:87108/1‑140 (MQ=255) ggCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCt < 2:70493/140‑1 (MQ=255) cGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCg > 1:34845/1‑139 (MQ=255) cGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCg < 2:34845/139‑1 (MQ=255) gCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTg > 2:576931/1‑140 (MQ=255) cccGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTacac > 1:813784/1‑140 (MQ=255) cccGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTacac > 2:689930/1‑140 (MQ=255) cgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCCTCTGCGTGCTGACCTTCTACACGCCg > 2:851702/1‑140 (MQ=255) tGCTCGACGCCGTCACCGACCCGCTGATGTGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa < 1:291924/140‑1 (MQ=255) tGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa < 2:518935/140‑1 (MQ=255) tCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACtt < 1:511291/140‑1 (MQ=255) gACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtct < 1:851702/140‑1 (MQ=255) gACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtct > 2:697552/1‑140 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 2:10327/140‑1 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 1:580461/140‑1 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 1:576931/140‑1 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 2:813784/140‑1 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 2:432774/140‑1 (MQ=255) cGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 2:467129/140‑1 (MQ=255) tCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGcaca < 2:480861/140‑1 (MQ=255) cACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca > 1:836114/1‑137 (MQ=255) cACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca < 2:836114/137‑1 (MQ=255) aCCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAgg < 2:97792/140‑1 (MQ=255) ccGCTGATGGGGCTGGTGGTAGTCCTCACGCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa < 1:357049/140‑1 (MQ=255) ccGCTGATGGGGCTGCTTGTAGACCGCACCCCCACCCGGTACGGCCAGCTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa < 2:301654/140‑1 (MQ=255) ccGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa < 1:14421/140‑1 (MQ=255) gggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTACTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACg < 2:490447/140‑1 (MQ=255) ggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGc > 2:122464/1‑140 (MQ=255) ggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGc < 1:689930/140‑1 (MQ=255) tgctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCCGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTg > 2:54360/1‑140 (MQ=255) gctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGc > 1:121006/1‑140 (MQ=255) tgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGt < 2:121006/140‑1 (MQ=255) tgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGt < 2:196157/140‑1 (MQ=255) tAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGAc < 2:439400/140‑1 (MQ=255) tAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGAc < 1:697552/140‑1 (MQ=255) tAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGAc < 1:169952/140‑1 (MQ=255) cgcacccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACa < 2:549310/140‑1 (MQ=255) gcacccgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACAt < 1:122464/140‑1 (MQ=255) cgcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTcc < 1:500911/140‑1 (MQ=255) cgGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAccc < 1:139006/140‑1 (MQ=255) aCGGCCAGTTCCGCCCGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGt < 1:744771/140‑1 (MQ=255) cccGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGTATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAccc < 2:30796/123‑1 (MQ=255) cccGTTCCTGCTGTGGG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGtt > 2:479340/1‑140 (MQ=255) cccGTTCCTGCTGT‑GGAGGGGCCATCCCGTTCGGTATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAccc > 1:30796/1‑123 (MQ=255) ccTGCTGTGGG‑GGGGGCATCCCTTTGGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTg < 1:346849/140‑1 (MQ=255) | TCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GG‑GGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTG > NC_000913/285504‑285772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |