| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,350,184 | (C)7→8 | coding (276/654 nt) | elbB ← | low activity glyoxalase ElbB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,350,177 | 1 | . | C | 85.7% | 120.3 / 13.5 | 42 | coding (283/654 nt) | elbB | low activity glyoxalase ElbB |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (22/14); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATG > NC_000913/3350038‑3350309 | agcaTCGCCGGGGCAATTCACATAAAACCAACCGGTTTTACGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑aaa < 2:348765/140‑1 (MQ=255) caTCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑cc < 1:737096/140‑1 (MQ=255) caTCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑cc < 1:101030/140‑1 (MQ=255) gCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑cccccc < 1:650724/140‑1 (MQ=255) gggCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑cccccc‑ccgg < 2:812857/140‑4 (MQ=255) gggCAATACACATAAAACCAAGCGGTTTTCCCGCCAGATCCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑cccccc‑ccgg < 2:249838/140‑4 (MQ=255) caTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAAc < 2:572015/140‑1 (MQ=255) aTAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACg > 2:86642/1‑140 (MQ=255) aaaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑C‑GGCACAATCAACGCAt > 1:174375/1‑140 (MQ=255) aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACaa > 2:544575/1‑131 (MQ=255) aaaCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACaa < 1:544575/131‑1 (MQ=255) aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCGAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATcc > 2:512081/1‑140 (MQ=255) aCCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATcc > 2:819076/1‑140 (MQ=255) aaGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGt > 1:825520/1‑140 (MQ=255) aGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGtt > 1:52521/1‑140 (MQ=255) gCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTc > 1:191412/1‑140 (MQ=255) gTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGc < 2:581702/140‑1 (MQ=255) tttCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTACGCCGCGCA‑AAA‑ACCCGC‑C‑GGCACAATCAAAGCATCCAGTTCAGCGGc > 2:274488/1‑140 (MQ=255) tCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCa < 2:575323/140‑1 (MQ=255) ccGGCCTGATGCATCTCTTGTGCCAACGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:279547/140‑1 (MQ=255) ccGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCAt < 2:52521/140‑1 (MQ=255) ccGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:86642/140‑1 (MQ=255) ccGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCAt < 1:515694/140‑1 (MQ=255) ccTGATGCATCGCTTCTGCCAGAGCCTTTTATTCACGATCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGcc < 1:274488/140‑1 (MQ=255) tGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑CAGGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCt > 1:319503/1‑140 (MQ=255) aTCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCa < 2:825520/140‑1 (MQ=255) cTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCTACGCATCTAGTTCAACGGCATAGGCCTGGGCCAGAgg > 1:315588/1‑140 (MQ=255) gcCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCAc < 2:191412/140‑1 (MQ=255) cTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACcg < 1:512081/140‑1 (MQ=255) gTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTc < 2:174375/140‑1 (MQ=255) gCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCa > 1:573839/1‑140 (MQ=255) aTTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTg < 1:696432/130‑1 (MQ=255) aTTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTg > 2:696432/1‑130 (MQ=255) tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCa > 2:446887/1‑140 (MQ=255) tCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCa > 2:94367/1‑140 (MQ=255) gCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGc > 2:564943/1‑140 (MQ=255) ggCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGtt > 1:591739/1‑140 (MQ=255) cAAAATTGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGAGCCCCTGCAATCAACACATTGCGCGTTTc > 1:384207/1‑140 (MQ=255) ttGCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGATTCAATGAGCACATTGCGCGTTTCCGTCa > 1:301917/1‑140 (MQ=255) gCTTAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATc > 1:98576/1‑140 (MQ=255) ttAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGTCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGc < 1:819076/140‑1 (MQ=255) ttAAATTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGc > 1:39207/1‑140 (MQ=255) aaaTTCTTCGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCtt < 2:98576/140‑1 (MQ=255) aaTTCTTCGCCGCGCT‑AAA‑CCCCCCGC‑GGTACAATCATCGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTc < 1:281376/140‑1 (MQ=255) ttcttcGCCGCGCCAAAA‑CCCCCC‑C‑GGCACACTCACCGCATCCAGTTCGGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGc < 2:384207/140‑1 (MQ=255) tcGCCGCGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCTTGCCGCTTCAATTAGCACATTGCGCTTTTCCCTCATCGCTTCGCCAGt > 2:281376/1‑140 (MQ=255) cgccgcGCC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGACGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGtt > 1:514699/1‑140 (MQ=255) cgcC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCGCATTGCGGGTTGCCGTCATCGCTTCACCAGTTAAATg > 1:389983/1‑140 (MQ=255) cgcC‑AAACCCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATg > 2:24621/1‑140 (MQ=255) | AGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCC‑AAA‑CCCCCC‑C‑GGCACAATCAACGCATCCAGTTCAGCGGCATCGGCCTGGGCCAGAGGACGGATTTCACCGCGCGTTATTCGTGCCGCTTCAATCAGCACATTGCGCGTTTCCGTCATCGCTTCGCCAGTTAAATG > NC_000913/3350038‑3350309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |