| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,215 | (G)8→10 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,207 | 1 | . | G | 90.0% | 82.9 / 3.6 | 30 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (13/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
| * | NC_000913 | 4,606,207 | 2 | . | G | 86.7% | 80.4 / 7.2 | 30 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (12/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GA‑‑G‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC > NC_000913/4606083‑4606320 || gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GA‑‑g‑‑gggg‑g‑‑ggggacttg > 1:818494/1‑133 (MQ=255) ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAG‑G‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCAc > 1:653909/1‑140 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTTCGCCACCTTCGCACCGTCATCTTACTTTTTTTGATATCCGCTTGTTTGGTGC‑‑GA‑‑GC‑GGGGAG‑‑GGA‑CTTG‑AACCCCCAc > 2:373448/1‑140 (MQ=18) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCAc > 2:520174/1‑140 (MQ=255) tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGGGCGTCTACCAAGTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGt < 2:172828/140‑1 (MQ=25) ngAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTAGCACAGTCATCTTACTTTTTTTGATATCGCCTCATTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGt < 2:499206/139‑1 (MQ=25) aCCCCCACGTCCGTAAGGACACTAATACCCGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAg < 1:373448/140‑1 (MQ=37) aCCCCCACGTCCGTAAGCACACTAACACCTGGAGCGAGCACGTCTACCCATTCCGCCGCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAg < 2:302467/140‑1 (MQ=25) ccAGGTCCGTAAGGAAACCAACACCGCAAGCTAACACAACTACCAAGTCCGCCACCTTAACACAGTAATATTACTTATTTTGAGATGGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAaca < 2:523031/140‑1 (MQ=19) aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACt < 1:520174/140‑1 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGATCCTACTTTTTTTGTTATCTCTGCGGTTGGTGC‑‑GG‑‑G‑‑GCGC‑G‑‑GGAGCCTGAAATCCCCACGGCCATGAGAACACTAACACCGCAAGcga > 2:348777/1‑138 (MQ=12) ccccTGAAGCTAGCGCGTCTACAAATTCCCCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGGG‑‑G‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg < 1:296470/138‑1 (MQ=25) ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGGGG‑‑G‑‑GGGG‑G‑‑GGA‑GTTG‑AACCCGCACGTCCATAAGAACATTAACACCTGAAGCTAGCGCGTc > 2:296470/1‑140 (MQ=37) gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc > 1:554593/1‑140 (MQ=255) cgcgTCTACCAATTCCGCCACCTTCGCCCAGTCCTCTCACTTTTTTTGATATCCCCCCGACGCGGGC‑‑GA‑‑G‑‑GGGC‑GACGGT‑GTGT‑AACCCCCGCGTCCGGACGAACCCTTAAACCAGAAGCTAGCGCGTCTACCAATTCCg > 2:745349/1‑140 (MQ=255) gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGG‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:554593/140‑1 (MQ=25) aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 1:653660/1‑140 (MQ=255) aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGTGC‑‑GAG‑G‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:653909/140‑1 (MQ=255) aTTCCGCCACCTTCGCACAGTCATCTTTCTTTTTTTGATATGGCCTCGGTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AGCCCCCGCGTCCGTAAGAACGCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:745349/140‑1 (MQ=21) tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTCGCCCa > 1:150185/1‑140 (MQ=255) cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt < 2:653660/140‑1 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:440553/134‑1 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 2:440553/1‑134 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt > 1:510802/1‑138 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt < 2:510802/138‑1 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc > 2:723828/1‑140 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc > 2:208678/1‑140 (MQ=255) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GA‑‑GGCGGGG‑G‑‑GGA‑CTTG‑GACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCACGTCAACCACTTCCGCCACCTCCGCCCaccgc > 1:503757/1‑136 (MQ=21) ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg < 1:593240/140‑1 (MQ=255) ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTT‑AACCCCCACGTCCCTAAAAACACTAACACCCGAAGCTATGTTGTCCACCAAATCCGCCCCCTTCCCCCCGTGGGGGCa > 2:642008/1‑140 (MQ=255) cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:771796/127‑1 (MQ=255) cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 2:771796/1‑127 (MQ=255) aacaGTCATCTTACTTCTTTTGGTAACGCCTAGTTCGGTCC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat < 2:395587/139‑1 (MQ=18) ttttttGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg > 1:434966/1‑140 (MQ=255) tttGATATCGCCTCGTTTGGTGC‑‑GAGGG‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGTGTTTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc > 2:127067/1‑140 (MQ=21) || GCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑‑GA‑‑G‑‑GGGG‑G‑‑GGA‑CTTG‑AACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGC > NC_000913/4606083‑4606320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |