| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 422,427 | (C)5→7 | intergenic (+68/‑91) | proY → / → malZ | putative transporter ProY/maltodextrin glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 422,422 | 1 | . | C | 97.7% | 147.8 / NA | 45 | intergenic (+63/‑96) | proY/malZ | putative transporter ProY/maltodextrin glucosidase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (15/28); minor base G (1/0); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 422,422 | 2 | . | C | 97.8% | 146.3 / ‑3.5 | 45 | intergenic (+63/‑96) | proY/malZ | putative transporter ProY/maltodextrin glucosidase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (16/28); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑‑C‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGC > NC_000913/422284‑422528 || cgcgTGGATTGTTGTTCTGTTGCTTAGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑‑c < 1:354995/140‑1 (MQ=255) gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑‑c‑‑c < 1:10822/140‑1 (MQ=255) ttgtGCTGTTGATTGGCGGGATTTTTAAACGCCGCCACGCTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑‑c‑‑cc‑‑ccccgtcct < 2:357102/140‑8 (MQ=255) gCTGTTGATTGGCTGCATGTTTCAACGCCCCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGtcctcctc < 1:237508/140‑1 (MQ=255) cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGtcctcctcc > 1:682582/1‑140 (MQ=255) tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCAATTACGAAAGTTATCCGGCC‑‑CC‑‑CCGTCCTCCTccc > 2:270066/1‑140 (MQ=255) ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATTCTGATCCGACTACTCCCAAAGTTATCCG‑‑CCTCC‑‑CCGTCCACCTccccc > 2:410516/1‑140 (MQ=255) ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCGATTCCGAAAGTAATACGCCC‑‑CC‑‑CCGtcctcctcctcc > 1:275155/1‑140 (MQ=255) tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAata > 1:37698/1‑140 (MQ=255) ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAatat < 2:797190/140‑1 (MQ=255) gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCAGGCTATTCCGAATGTTGGCCG‑‑C‑‑CCCTCCGTTCTCCTCCCCCAAATATc > 2:410964/1‑140 (MQ=255) cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATcc > 2:144274/1‑140 (MQ=255) ggATGTTTAAACGCCGCTACGAGCGTCAGCTCGCTAAAATCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCtt < 2:309008/140‑1 (MQ=255) ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCtt < 2:18850/140‑1 (MQ=255) aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCa < 2:539513/140‑1 (MQ=255) gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAga < 2:682582/140‑1 (MQ=255) ttAAACGCCGCCACGATTGTCACCTGGCTGACAACTAGTTATCCTTGCATTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAgatg < 2:268010/140‑1 (MQ=255) tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAgatga > 2:716470/1‑140 (MQ=255) tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCCCCTCCCCCAAATACCCCTctcttga > 2:156840/1‑134 (MQ=255) cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg < 2:708454/140‑1 (MQ=255) cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg < 1:58079/140‑1 (MQ=255) cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg < 1:632553/140‑1 (MQ=255) cgccgcCACGATCGTCAGCTGGATGCAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg < 1:410516/140‑1 (MQ=255) gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGt > 2:161820/1‑140 (MQ=255) cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATcc > 1:441950/1‑124 (MQ=255) cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATcc < 2:441950/124‑1 (MQ=255) ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc < 2:37698/140‑1 (MQ=255) ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc < 1:144274/140‑1 (MQ=255) ccACGATCGTCAGCTAGCTGAAGACGAGTGATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc < 1:410964/140‑1 (MQ=255) gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa > 2:450247/1‑140 (MQ=255) cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg < 1:101839/140‑1 (MQ=255) cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg < 1:727119/140‑1 (MQ=255) aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc < 1:161820/140‑1 (MQ=255) tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa < 1:156840/140‑1 (MQ=255) cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt < 1:606648/140‑1 (MQ=255) cAGTAATCCTTGCGTTCCGTAATCCGGTTCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCTCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt < 2:275155/140‑1 (MQ=255) tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca > 2:215829/1‑140 (MQ=255) gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca < 1:215829/140‑1 (MQ=255) cTGTAAGCCGGTTCAGGAGGAACTCCTGACCCGGCTAGTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg < 1:307152/140‑1 (MQ=255) cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg < 1:736759/140‑1 (MQ=255) tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGCGTGATCCTTCATCAGGTTATGGCAATGTGATCAGATTTTCATCACAgg > 2:307152/1‑140 (MQ=255) tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca > 2:434764/1‑138 (MQ=255) tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca < 1:434764/138‑1 (MQ=255) gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg < 1:450247/140‑1 (MQ=255) ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg < 2:185676/129‑1 (MQ=255) ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg > 1:185676/1‑129 (MQ=255) ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga > 1:544756/1‑140 (MQ=255) aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa < 2:130915/140‑1 (MQ=255) aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCC‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTactgtc > 1:498951/1‑135 (MQ=255) || CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCG‑‑C‑‑CC‑‑CCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGC > NC_000913/422284‑422528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |