| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 915,319 | (A)7→8 | intergenic (‑462/+33) | lysO ← / ← aqpZ | L‑lysine exporter/water channel AqpZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 915,312 | 1 | . | A | 93.5% | 99.4 / ‑0.2 | 31 | intergenic (‑455/+40) | lysO/aqpZ | L‑lysine exporter/water channel AqpZ |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (20/9); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑T‑AAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATG > NC_000913/915196‑915446 | tGCAGGGGGCGGCGGAGATTGGGCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGc < 1:285120/140‑1 (MQ=37) cggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAAGATCT‑T‑AAAAAAGAGCCCTGACGTTACGCCAGGCCTTc > 1:299507/1‑140 (MQ=37) ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCCCCAATGGAAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCg < 2:273150/140‑1 (MQ=38) gccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa > 1:6840/1‑140 (MQ=255) cccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTATCTCATGAATGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc < 2:258741/140‑1 (MQ=255) tGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGt > 1:127817/1‑140 (MQ=255) tGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGt > 2:126890/1‑140 (MQ=255) tGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCg > 1:559671/1‑140 (MQ=255) cTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGAt > 2:781877/1‑140 (MQ=255) cTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAACAGGGTCCGGTAAATCAGACCACCGAt > 2:583156/1‑140 (MQ=255) ttAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATa > 2:792758/1‑140 (MQ=255) aGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAAt > 1:845635/1‑140 (MQ=255) aGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCGTTTCCAGAAGGGTCGGGTAAAGCAGACCACCGATAAt > 1:416544/1‑140 (MQ=255) cTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCATTTGCAGGAGGGTCCGATAAAGCAGACCACCGATAATgc > 2:236932/1‑140 (MQ=255) caacaaGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGca < 1:14743/140‑1 (MQ=255) caacaaGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGca < 1:126890/140‑1 (MQ=255) caacaaGATGATGAATCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGca < 2:6840/140‑1 (MQ=255) gatgaATCAACATATCACAACATCTCA‑AAAAAA‑AGGCGTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCaga < 1:236932/140‑1 (MQ=255) aaTCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAgaagaa > 1:70072/1‑140 (MQ=255) tCAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAAcc > 2:812511/1‑140 (MQ=255) cAACATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACca < 2:559671/140‑1 (MQ=255) aaCATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACcac < 2:845635/140‑1 (MQ=255) aCATATAACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACcaca > 1:847612/1‑140 (MQ=255) taACAACATCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAgttgt > 2:487255/1‑140 (MQ=255) caTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAgtt < 2:685154/132‑1 (MQ=255) caTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAgtt > 1:685154/1‑132 (MQ=255) caTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAAt > 2:649182/1‑140 (MQ=255) caTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAAt > 2:45346/1‑140 (MQ=255) caTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAAt > 1:178017/1‑140 (MQ=255) aTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATg > 2:567054/1‑140 (MQ=255) aTCT‑TAAAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATg > 1:790038/1‑140 (MQ=255) | TGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCT‑T‑AAAAAA‑AGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATG > NC_000913/915196‑915446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |