Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,237,673	(G)5→6	coding (103/1299 nt)	dadA →	D‑amino acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,237,668	1	.	G	100.0%	214.6 / NA	63	P33R (CCG→CGG)	dadA	D‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (36/27);  total (36/27)

ATTATTATTCTTTTACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCC‑‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTC  >  NC_000913/1237530‑1237802                                                                                                                                            |                                                                                                                                        attattattCTTTTACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCC‑‑g                                                                                                                                       <  1:685882/140‑1 (MQ=255)      tattCTTTTACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCC‑‑gggggg                                                                                                                                  >  1:796887/1‑139 (MQ=255)           ttttACTGTATCTACTGTTATCAGATTGGCTATGCGACTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGgccgcc                                                                                                                              <  1:243337/140‑1 (MQ=255)           ttttACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGAGATGTGGTTACCGTCATGGATCGGGAGCCG‑GGGGgccgcc                                                                                                                              >  2:371371/1‑140 (MQ=255)           ttttACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACAGGAGGTCAACGTCATCGATCGGTAGCCG‑GGGGgccgcc                                                                                                                              >  1:305843/1‑140 (MQ=255)             ttACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCt                                                                                                                            >  2:424767/1‑140 (MQ=255)              tACTGTATCTACCGTTATCGGATTGGCTATGGGAGGTGTTATACTGGGCAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTg                                                                                                                           <  2:305843/140‑1 (MQ=255)               aCTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTgg                                                                                                                          >  2:539350/1‑140 (MQ=255)               aCTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTgg                                                                                                                          >  2:562724/1‑140 (MQ=255)                     tCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCa                                                                                                                    >  2:498312/1‑140 (MQ=255)                          cGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGcc                                                                                                               >  2:664382/1‑140 (MQ=255)                           gTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGgccgcc                                                                                                                              >  2:42878/1‑124 (MQ=255)                           gTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGgccgcc                                                                                                                              <  1:42878/124‑1 (MQ=255)                             tATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAGTCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCcgcg                                                                                                            >  1:500595/1‑140 (MQ=255)                             tATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTAATCGGGACCCG‑GGGGGACGCCCTGGAAACCAGTGCcgcg                                                                                                            >  2:346359/1‑140 (MQ=255)                               tCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGaa                                                                                                          >  1:726999/1‑140 (MQ=255)                               tCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGaa                                                                                                          >  1:210040/1‑140 (MQ=255)                               tCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGaa                                                                                                          >  1:432048/1‑140 (MQ=255)                                 ggAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATg                                                                                                        <  2:210040/140‑1 (MQ=255)                                       gCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGGCCTGGAAACCAGTGCCGCGAATGCCGGGc                                                                                                  >  1:340273/1‑140 (MQ=255)                                       gCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGc                                                                                                  >  2:591554/1‑140 (MQ=255)                                           tGCGAGTTGTCATACGGGGAAGGGGGGTGGTAGGCGTCGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAAt                                                                                              <  1:346359/140‑1 (MQ=255)                                             cGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAAtct                                                                                            >  2:179965/1‑140 (MQ=255)                                               aGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTcc                                                                                          <  1:424767/140‑1 (MQ=255)                                                 ttGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCTTGGAAACAAGTGCCGCGAATGCCGGGCAAATCTcccc                                                                                        >  1:343868/1‑140 (MQ=255)                                                  tGTCATACTGGGAAGTGGTGTGCTAGGCGCTGCCAGCCCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTccccc                                                                                       <  1:374899/140‑1 (MQ=255)                                                    tCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     <  1:664382/140‑1 (MQ=255)                                                    tCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     <  1:179965/140‑1 (MQ=255)                                                    tCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     <  2:726999/140‑1 (MQ=255)                                                    gcATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     <  2:582112/139‑1 (MQ=255)                                                      aTACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGat                                                                                   >  1:491743/1‑140 (MQ=255)                                                            ggAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     <  2:488095/132‑1 (MQ=255)                                                            ggAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCgg                                                                                     >  1:488095/1‑132 (MQ=255)                                                            ggAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGAGCCGCGACTGCGGGGCAAATCCCCCCCGGATAtgctg                                                                             >  1:336772/1‑140 (MQ=255)                                                              aaGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCa                                                                           >  2:534678/1‑140 (MQ=255)                                                              aaGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCa                                                                           >  2:863475/1‑140 (MQ=255)                                                                 tggtgtggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGATACCAGTGCAGCGCAAGACGGGCAAAACTCCATCGGATATGCTGCACCg                                                                        >  2:422540/1‑140 (MQ=255)                                                                 tggtgtggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCg                                                                        >  2:480968/1‑140 (MQ=255)                                                                 tggtgtggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCAGCGAATGGCGGGCAAAGCTCCCCCGGATATGCTGCACCg                                                                        >  2:273488/1‑140 (MQ=255)                                                                    tgtggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTgg                                                                     <  1:591554/140‑1 (MQ=255)                                                                      tggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGggc                                                                   <  1:187834/140‑1 (MQ=255)                                                                      tggtAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGggc                                                                   >  1:82644/1‑140 (MQ=255)                                                                          aGGCGTTGCCAGCGCATGGTACATACATCTGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCa                                                               <  1:371371/140‑1 (MQ=255)                                                                           ggCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATC‑CCCCCGGATATGCTGCACCGTGGGCGGCAcc                                                             <  1:498312/140‑1 (MQ=255)                                                                             cGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCa                                                            >  1:758084/1‑140 (MQ=255)                                                                                tGCCAGCGCCTGGTAGTTAAATCAGGCAGCACATGAGCTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGgt                                                         <  1:326205/140‑1 (MQ=255)                                                                                          tGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTaa                                               <  1:562724/140‑1 (MQ=255)                                                                                           ggTACTTAAATCAGGTAGGACACGAGGTCCCCGTCATGGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTaaa                                              <  1:422540/140‑1 (MQ=255)                                                                                           ggTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTaaa                                              <  1:480968/140‑1 (MQ=255)                                                                                             tACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAg                                            <  2:758084/140‑1 (MQ=255)                                                                                                ttAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGa                                         <  2:432664/140‑1 (MQ=255)                                                                                                   aaTCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTa                                      <  1:534678/140‑1 (MQ=255)                                                                                                       aggcaggACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATg                                  >  2:553603/1‑140 (MQ=255)                                                                                                       aggcaggACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATg                                  >  2:441628/1‑140 (MQ=255)                                                                                                           aggACATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATg                              <  1:539350/140‑1 (MQ=255)                                                                                                             gACATGAGGTCACCGCCATTGACCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGtt                            <  1:273488/140‑1 (MQ=255)                                                                                                              aCATGAGGTCACCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGGGTGCCTTTAAAAGCGATTAAATGGATGTTc                           >  1:623152/1‑140 (MQ=255)                                                                                                                       cACCGTCATTGATCGGGAGCCGGGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTGAAAACGATTAAATAGATGTTCCAGCGCCa                   >  1:332390/1‑140 (MQ=255)                                                                                                                        aCCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATg                 >  1:77175/1‑140 (MQ=255)                                                                                                                        aCCGTCATTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATg                 >  1:467851/1‑140 (MQ=255)                                                                                                                              aTTGATCGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGcgccgc           >  2:662662/1‑140 (MQ=255)                                                                                                                                    cGGGAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCgg     <  1:553603/140‑1 (MQ=255)                                                                                                                                      ggAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGtt   >  2:195957/1‑140 (MQ=255)                                                                                                                                      ggAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGtt   <  1:195957/140‑1 (MQ=255)                                                                                                                                      ggAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGtt   >  2:94915/1‑140 (MQ=255)                                                                                                                                       gAGCCG‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTc  >  2:865221/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        ATTATTATTCTTTTACTGTATCTACCGTTATCGGAGTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCC‑‑GGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTC  >  NC_000913/1237530‑1237802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑