Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,875	A→G	V513V (GTA→GTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,875	0	A	G	100.0%	128.8 / NA	37	V513V (GTA→GTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

GATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACT  >  NC_000913/1746‑2001                                                                                                                                  |                                                                                                                               gATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTa                                                                                                                      <  1:783632/140‑1 (MQ=255)      gCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAATTCGCAGGCTCTGCTCAACAATGTGCATGGCCtctgtctc                                                                                                                 >  2:363434/1‑133 (MQ=255)      gCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTg                                                                                                                 >  2:690344/1‑140 (MQ=255)      gCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTg                                                                                                                 >  2:36910/1‑140 (MQ=255)      gCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTg                                                                                                                 >  1:495708/1‑140 (MQ=255)           ggTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGaaaa                                                                                                            <  1:697833/140‑1 (MQ=255)           ggTGGCGTTGGCGGTCCGCTGCTGGCGCAACTGAAGCTTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGaaaa                                                                                                            <  1:280298/140‑1 (MQ=255)           ggTGGCGTTGGCGGTCCGCTGCCGGCGCAACTGAAGCTTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGaaaa                                                                                                            <  1:413034/140‑1 (MQ=255)                    ggCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGga                                                                                                   <  2:667575/140‑1 (MQ=255)                    ggCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGga                                                                                                   <  2:495708/140‑1 (MQ=255)                         tGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAAc                                                                                              <  2:713522/140‑1 (MQ=255)                               gctgGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGgcgc                                                                                        <  2:669519/140‑1 (MQ=255)                                         cTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAaga                                                                              >  1:691769/1‑140 (MQ=255)                                          tGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag                                                                             <  1:444852/140‑1 (MQ=255)                                          tGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag                                                                             <  2:850720/140‑1 (MQ=255)                                          tGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAagag                                                                             >  1:22708/1‑140 (MQ=255)                                              gCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGt                                                                         <  1:36910/140‑1 (MQ=255)                                                 tCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTa                                                                      >  1:114471/1‑140 (MQ=255)                                                   aGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAAt                                                                    >  1:683358/1‑140 (MQ=255)                                                        aGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCgg                                                               <  1:690344/140‑1 (MQ=255)                                                         gctggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGTAGAACTGACGCCAGCCAAAGAACCGTTTAATCTCggg                                                              >  1:320694/1‑140 (MQ=255)                                                           tggctgAAGAATAAACATATCGACTTACGTGCCTGGGGTGTTGCCAACGCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGgcg                                                            <  2:355357/140‑1 (MQ=255)                                                                     aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGGAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGc                                                  <  1:160388/140‑1 (MQ=255)                                                                      tAAACATATCGACTTACATGTCCGCGGTGTTGCCACCTCCAAGGCTCTTCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGcc                                                 <  2:287518/140‑1 (MQ=255)                                                                         aCATATCGACTTACGTGCCTGCGGTGTCGCCTACGCGAACGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCg                                              <  2:315993/140‑1 (MQ=255)                                                                            tatCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGa                                           <  2:691769/140‑1 (MQ=255)                                                                                gACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGa                                       >  1:638385/1‑140 (MQ=255)                                                                                       gtgtCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatcat                                <  2:683358/140‑1 (MQ=255)                                                                                            tGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgct                           >  2:176688/1‑140 (MQ=255)                                                                                                gtgtTGCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCATAATTCGCCTCGTGAAAAAATATCATCTGCTGAAc                       >  2:310990/1‑140 (MQ=255)                                                                                                    tGCCAACTCGAAGGCGCTGCTCTCCGATGCGCATGGCGTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCgg                   <  1:359987/140‑1 (MQ=255)                                                                                                     gCCAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGt                  >  1:79195/1‑140 (MQ=255)                                                                                                      ccAACTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTCATTGGCCTCGTGAAAGCATATCATCTGCTGAACCCGGTc                 >  2:359987/1‑140 (MQ=255)                                                                                                         aCTCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCAtt              <  2:638385/140‑1 (MQ=255)                                                                                                           tCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCgg                   <  1:75678/133‑1 (MQ=255)                                                                                                           tCGAAGGCTCTGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCgg                   >  2:75678/1‑133 (MQ=255)                                                                                                                     tGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACt  >  2:119747/1‑140 (MQ=255)                                                                                                                     tGCTCACCAATGTGCATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAACCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACt  <  1:802533/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                               GATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACT  >  NC_000913/1746‑2001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑