Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	202,893	G→A	G112S (GGC→AGC)	lpxA →	UDP‑N‑acetylglucosamine acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	202,893	0	G	A	100.0%	131.8 / NA	40	G112S (GGC→AGC)	lpxA	UDP‑N‑acetylglucosamine acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/15);  total (25/15)

CTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGAC  >  NC_000913/202757‑203029                                                                                                                                         |                                                                                                                                         cTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCATGGCGGTGGATTGACGAAGGTGAGCa                                                                                                                                       >  1:344099/1‑140 (MQ=255)  tCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAg                                                                                                                                      >  2:565338/1‑140 (MQ=255)   ccATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGagcagc                                                                                                                                     <  1:197976/140‑1 (MQ=255)     aTCGGCGAAGTTAACCATGATCTGAAATATGCTGGTGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGa                                                                                                                                   <  2:312922/140‑1 (MQ=255)     aTCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGa                                                                                                                                   <  1:796028/140‑1 (MQ=255)      tCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGAc                                                                                                                                  >  1:805561/1‑140 (MQ=255)                           tGAAATATGCTGGCGAAGCGACTCGTGTGGACAGCCGCGATCGTAACCCCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAAcgcg                                                                                                             <  2:387981/140‑1 (MQ=255)                               atatGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGcaca                                                                                                         <  2:53226/140‑1 (MQ=255)                                  tGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTg                                                                                                      >  1:863381/1‑140 (MQ=255)                                  tGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTg                                                                                                      >  2:708247/1‑140 (MQ=255)                                  tGCTCGCGAACCGACCCGTCTGGAACTCGCAGTTCGTAACAGCATTCGCGAAAGCGTCACCATGCATAGCGGCACAGCCCAGGGCGGAGGGTTGTCGATGGAGAGCAGCGTCAACTTACTGCTGATCAACGCGCACATTg                                                                                                      >  2:785324/1‑140 (MQ=255)                                   gCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACATACTGATGAACAACGCGCACATTgc                                                                                                     >  1:337741/1‑140 (MQ=255)                                    cTGGCGAACCGACCCGTGTGGACATCGGCGACCGTTACCGCATTCGCGTAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCGACGCGCACATTgcg                                                                                                    <  2:369254/140‑1 (MQ=255)                                    cTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTgcg                                                                                                    <  1:565338/140‑1 (MQ=255)                                    cTGGCGAACCGACCCGTGTGGAAATCGGCGATCATAACCGCATTCGCGAAAGCGTAACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTgcg                                                                                                    <  2:1363/140‑1 (MQ=255)                                      ggCGAACCTACCCGTGACGAAAACGGCGATCTTAATCGCTTTCGCGAATGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCa                                                                                                  <  2:420787/140‑1 (MQ=255)                                       gCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCAc                                                                                                 >  2:189038/1‑140 (MQ=255)                                          aaCCGACCCGTGTGGAAATCGGCGATCGTAACCGGATTCGCGAACGGGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGAt                                                                                              <  1:785324/140‑1 (MQ=255)                                                 ccGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTTCTGATGAACAATGCTCACATTACGCACGATTGTACgg                                                                                       >  1:407214/1‑140 (MQ=255)                                                        gAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTaa                                                                                <  2:805561/140‑1 (MQ=255)                                                         aaaTCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAAc                                                                               >  1:639363/1‑140 (MQ=255)                                                              ggCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTg                                                                          >  2:17132/1‑140 (MQ=255)                                                                     gTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                   <  1:708247/140‑1 (MQ=255)                                                                          cGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCcaa                                                              <  1:17132/140‑1 (MQ=255)                                                                             aTTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                   >  2:63641/1‑132 (MQ=255)                                                                             aTTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATtctc                                                                   <  1:63641/132‑1 (MQ=255)                                                                              ttCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCaacaac                                                          >  1:601340/1‑140 (MQ=255)                                                                                cgcgAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAAcaacgc                                                        >  1:707701/1‑140 (MQ=255)                                                                                         gTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCTACAACTTACTGATGATCAACGCGCACATTGCGCAAGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGc                                               <  2:707701/140‑1 (MQ=255)                                                                                                          cacaGTCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTg                              >  1:657091/1‑140 (MQ=255)                                                                                                               tCCAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTgacgac                         >  1:622531/1‑140 (MQ=255)                                                                                                                ccAGGGCGGTGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACt                        <  1:189038/140‑1 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGatca               >  1:732476/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGatca               >  1:485730/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCCGCTGAAGACTTCGCGatca               >  1:690659/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGGATCGGGTGACGACCTCGCGctca               >  2:708654/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGGCACGGATCGGGTGACGACTTCGCGatca               >  2:24108/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGATCACGGCTCGGTTAGCGGCTTCTCGatca               >  1:423017/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCGGGCGGGTCACGTCTCGGATGACGACTTCGCGaaca               >  2:304305/1‑140 (MQ=255)                                                                                                                         tGGATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACACTGGCGGCTCAAGTATCGGTTGACGACTTCGCGatca               >  1:345929/1‑140 (MQ=255)                                                                                                                           gATTGACGAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGatcatc             >  2:528520/1‑140 (MQ=255)                                                                                                                                  gAAGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCa      <  2:583732/140‑1 (MQ=255)                                                                                                                                   aaGGTGAGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGGCACGTATCGGGTGACGACCTCGCGATCATCCGGGGCAt     >  2:850615/1‑140 (MQ=255)                                                                                                                                      gTGAGCAGTGACAACTTTCTGATGATCAACGAGAAAATTGCGCACGATAGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGAc  <  2:423017/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAACGCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGAC  >  NC_000913/202757‑203029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑