| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,535,988 | C→T | A210A (GCG→GCA) | narV ← | nitrate reductase Z subunit gamma |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,535,988 | 0 | C | T | 95.1% | 130.1 / NA | 41 | A210A (GCG→GCA) | narV | nitrate reductase Z subunit gamma |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (22/17); minor base G (2/0); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGC > NC_000913/1535855‑1536121 | tCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAACTTGATATTGACTAGGAAAGTAGTCAAACGGTGCGCTc > 1:367093/1‑140 (MQ=255) cATTAACGATCTCCTGCTACTGGAGAACTAGTAAAACCTCCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTcc < 2:416224/140‑1 (MQ=255) aCGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCAACGAGTAAAGTACTCAAACGGTGCGCTCcacaca < 1:439897/140‑1 (MQ=255) tctcCTGTGACTGGATAACTATAATAGCCAGCAAGTGTCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCa < 1:392747/140‑1 (MQ=255) ctcCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAAGTTGATATCGACGTGTATAGTACTCAGACGGGGCGCTCCACACATGCAc > 2:236831/1‑140 (MQ=255) aCTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCggg > 2:96790/1‑140 (MQ=255) tGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTg > 1:189569/1‑140 (MQ=255) gaACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGGGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACg < 1:96790/140‑1 (MQ=255) aCTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACggg < 1:17111/140‑1 (MQ=255) cTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGa < 2:6239/140‑1 (MQ=255) tATCATAGCCTGCAAGTGGCCGGAGCGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAGCGGGGCGGTCCACACAGGCACAAGTCGGGGGAACGGGaa > 2:234864/1‑140 (MQ=255) tCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAaga > 2:662079/1‑140 (MQ=255) gCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCTCTCGAAACATGCACCAATCGGCTGAACGGGAAGAGCAgg > 2:398925/1‑140 (MQ=255) cTGCAAGTGGCCGGATAGCGAATGGCTATGCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGaa < 1:277553/140‑1 (MQ=255) tGCAAGTGTCCGGAGAGCGAAGGGCTATCCGGCCAGGGGGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGCCGGGTGAACGGCAAGAgcaggcag > 2:184575/1‑140 (MQ=255) tGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAg > 2:614303/1‑140 (MQ=255) cAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGAt > 1:94792/1‑140 (MQ=255) ggCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCa < 1:459309/140‑1 (MQ=255) ggAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTcc < 2:189569/140‑1 (MQ=255) agagCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCGGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCa > 1:852526/1‑140 (MQ=255) agagCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCa > 1:849512/1‑140 (MQ=255) tATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCa > 1:470583/1‑140 (MQ=255) ggCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCgg < 2:849512/140‑1 (MQ=255) ccAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAACAGAAAGGTGGTCATTGCCAAGACCAGATGCAGGCGGaa > 2:280417/1‑140 (MQ=255) ggTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACg > 2:683108/1‑140 (MQ=255) aaTTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCTGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGc > 2:217261/1‑140 (MQ=255) aTTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCt > 2:160646/1‑140 (MQ=255) ttATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTGATTCCCAACACCAGGTGCAGGCGGAAGACGAACGCTa > 1:396327/1‑140 (MQ=255) ttATCGCCGCGAACGCAAAATTCGATATCGAGGAGTAAAATACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTa < 2:367093/140‑1 (MQ=255) gccgcGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCg < 1:683108/140‑1 (MQ=255) aTTTGATATCGACGACTAAAGTACTGAAACGGTGCGCACCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGa < 1:398925/140‑1 (MQ=255) gATATCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGAt < 2:470583/140‑1 (MQ=255) atatCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATc > 2:466204/1‑140 (MQ=255) atatCGACGAGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATc > 1:495786/1‑140 (MQ=255) atatCGACGAGTAAAGTACCCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAg < 1:814633/126‑1 (MQ=255) atatCGACGAGTAAAGTACCCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAg > 2:814633/1‑126 (MQ=255) atatCGACGAGTAAACTACTCATACCGTGTGCTCCACCCATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATc < 1:234864/140‑1 (MQ=255) cgacgaGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATccacc > 1:23199/1‑140 (MQ=255) cgacgaGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATccacc > 2:697211/1‑140 (MQ=255) gacgaGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATccacca < 1:662079/140‑1 (MQ=255) acgaGTAAAGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCcaccac > 1:709405/1‑140 (MQ=255) aGTACTCAAACGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTg > 2:715338/1‑140 (MQ=255) aaaCGGTGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGc > 2:852045/1‑140 (MQ=255) | TCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTGAAATTATCGCCGCGAACGCACAATTTGATATCGACGAGTAAAGTACTCAAACGGCGCGCTCCACACATGCACCAGTCGGGTGAACGGGAAGAGCAGGAAGATGGTCATTCCCAACACCAGATGCAGGCGGAAGACGAACGCTACGCCGTTGAGCATCTCTGACGATCCACCACGGAAAGTGACAATGC > NC_000913/1535855‑1536121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |