Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,564,808	T→C	H309R (CAC→CGC)	dosP ←	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,564,808	0	T	C	100.0%	116.5 / NA	36	H309R (CAC→CGC)	dosP	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/23);  total (13/23)

TATCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTG  >  NC_000913/1564674‑1564933                                                                                                                                       |                                                                                                                              tatCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGGCCAGCTTGGCGTATGTTGAATGTCTGCACCGCGGGaa                                                                                                                          >  1:223953/1‑140 (MQ=255) tatCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACGACCTTTGCGCATATTGAATTTCTGCACCGCGGGaa                                                                                                                          >  2:422520/1‑140 (MQ=255) tatCTGCCACGCGTTCGATAAAGGCGCTGGCTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGCCCAGCTTTGCGGATATTGAATGTCTGCACCGCGGGaa                                                                                                                          >  1:355475/1‑140 (MQ=255)      gCCACGCGTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCCTTTCGAATTTCTGTACCGCGGGAAGATGa                                                                                                                     >  1:386926/1‑140 (MQ=255)            cgTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGGATTACTGCACCGCTGGAAGATGACGCCCa                                                                                                               >  1:227204/1‑140 (MQ=255)                       ggCGCTGGTTTCTGCTCCTGACGAGGTTTTATTTTCCAGGATCCCCGCTGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCa                                                                                                    <  2:227204/140‑1 (MQ=255)                       ggCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCa                                                                                                    <  1:668425/140‑1 (MQ=255)                         cgcTGGTTTCTGCTCCCGACGATGTTTTAATGTCCTGGATCCCCGCAGTCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATc                                                                                                  <  2:380588/140‑1 (MQ=255)                                     cTCCTGACGAGGTTTAAATTTGCAGCATCCCCGCAGGTGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGt                                                                                      <  1:225484/140‑1 (MQ=255)                                        cTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCg                                                                                   >  2:111955/1‑140 (MQ=255)                                        cTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCg                                                                                   <  1:502641/140‑1 (MQ=255)                                                          gCAGGATCCTCGAAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGAt                                                                 <  2:541178/140‑1 (MQ=255)                                                          gCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGAt                                                                 <  1:834303/140‑1 (MQ=255)                                                          gCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGAt                                                                 >  1:605932/1‑140 (MQ=255)                                                          gCAGGATCCCCGCAGCCGCGCCCTCCCGCAGACGAATTGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGAt                                                                 <  1:219492/140‑1 (MQ=255)                                                             ggATCCCCGCAGGCGGGCCATCTCGGTGACGAATGGTGGATGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCg                                                              <  1:331431/140‑1 (MQ=255)                                                               aTCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGtt                                                            <  1:824995/140‑1 (MQ=255)                                                                    cGCAGGCGCGCCATCACTCTGAGGAATGGTCGCTGGCCATCTTTGCGCTTTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTa                                                       <  2:305242/140‑1 (MQ=255)                                                                       gcaggcgcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCCTCCCGTTGCGCACTACTCACAGCGAAACATGGGATTCGTCGAGTACAg                                                    >  2:402577/5‑140 (MQ=255)                                                                         gcgcgcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGAt                                                  >  2:543500/1‑140 (MQ=255)                                                                          cgcgcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGAtt                                                 >  2:485537/1‑140 (MQ=255)                                                                           gcgcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTc                                                >  2:790410/1‑140 (MQ=255)                                                                           gcgcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTc                                                <  1:651828/140‑1 (MQ=255)                                                                           gcgcCATCACGCTGAAGAATGGCCGCTGAGCAGCTTTTCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTc                                                <  1:422520/140‑1 (MQ=255)                                                                             gcCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGa                                              >  1:121992/1‑140 (MQ=255)                                                                                aTCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGt                                           <  1:485537/140‑1 (MQ=255)                                                                                        gACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACaa                                   <  2:605932/140‑1 (MQ=255)                                                                                        gACGAATGGTCGCTGACCAGCTGTGCGCATTTTGAATCTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTGGATGTTACGACaa                                   <  1:372769/140‑1 (MQ=255)                                                                                        gACGAATGGTCGCTGACCAGCTCTGCGCATTTTGAGTTTGTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACaa                                   <  2:298544/140‑1 (MQ=255)                                                                                         aCGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACaaa                                  >  1:579001/1‑140 (MQ=255)                                                                                           gAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATg                                <  1:188946/140‑1 (MQ=255)                                                                                             aTGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt                              <  2:579001/140‑1 (MQ=255)                                                                                                  cGCTGACCAGCTTTGCATATTTAGAATTTCTTCCCTGCGGGAAGATGAGGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTccc                         <  2:394167/140‑1 (MQ=255)                                                                                                   gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGCTGAGTACAGATACGGTGTTACAACAAATGATTTcccc                        >  1:298544/1‑140 (MQ=255)                                                                                                                    aTTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCg       <  2:121992/140‑1 (MQ=255)                                                                                                                    aTTTTGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCg       <  1:543500/140‑1 (MQ=255)                                                                                                                      tttGAATTTCTGCACTGCGGGACGACGACACCCAGTGCATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCggg     <  1:402577/140‑1 (MQ=255)                                                                                                                      tttGAATTTCTGCACCGCGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCggg     <  1:111955/140‑1 (MQ=255)                                                                                                                         gAATTTCTGCACCGCGGCAAGACGACGCCCATTGTTTCGCCATCCCGTCGCGCAGTGCGAACAGCGAAAGATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTGCATGAAATGGCGGgctg  <  2:355475/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              TATCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGCTCCTGACGAGGTTTTAATTTGCAGGATCCCCGCAGGCGCGCCATCACGCTGACGAATGGTCGCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATTTCCCCCATTTCATGAAATGGCGGGCTG  >  NC_000913/1564674‑1564933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑