Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,596,091	A→G	pseudogene (1996/5422 nt)	yneO ←	AIDA‑I family autotransporter YneO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,596,091	0	A	G	100.0%	277.8 / NA	79	pseudogene (1996/5422 nt)	yneO	AIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/35);  total (44/35)

TGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCGTAC  >  NC_000913/1595954‑1596225                                                                                                                                          |                                                                                                                                       tGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCt                                                                                                                                      >  1:614497/1‑140 (MQ=255) tGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCt                                                                                                                                      >  1:581216/1‑140 (MQ=255)   cATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTCGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTcc                                                                                                                                    >  1:808067/1‑140 (MQ=255)    aTTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCa                                                                                                                                   >  2:85837/1‑140 (MQ=255)        aaCACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACttt                                                                                                                               >  1:672715/1‑140 (MQ=255)        aaCACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATGGGTATGATGAAAAACAAAAACGCCTGCGCCAAGACCAAGCTCCACttt                                                                                                                               >  2:266311/1‑140 (MQ=255)             cGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACTAAAACGCCTTCACCAAGACCAGGCTCCACTTtcgtcg                                                                                                                          >  1:339442/1‑140 (MQ=255)             cGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCTAGACCAAGCTCCACTTtcgtcg                                                                                                                          >  1:293094/1‑140 (MQ=255)             cGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTtcgtcg                                                                                                                          >  2:833150/1‑140 (MQ=255)             cGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTtcgtcg                                                                                                                          >  2:187110/1‑140 (MQ=255)               tATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCa                                                                                                                        <  1:91111/140‑1 (MQ=255)                  ggCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCa                                                                                                                        >  2:759510/1‑137 (MQ=255)                  ggCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCa                                                                                                                        >  1:669873/1‑137 (MQ=255)                  ggCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCa                                                                                                                        <  1:759510/137‑1 (MQ=255)                  ggCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCa                                                                                                                        <  2:669873/137‑1 (MQ=255)                   gCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTgg                                                                                                                    <  2:502184/140‑1 (MQ=255)                     cTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTg                                                                                                                  <  1:658087/140‑1 (MQ=255)                       gCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGAGAAAAACGCCTTCGCCAGGACCAAGCCCCACATTCGTCGCATTGGTGAt                                                                                                                >  2:275337/1‑140 (MQ=255)                       gCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGAt                                                                                                                >  2:428458/1‑140 (MQ=255)                       gCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGAt                                                                                                                >  1:540381/1‑140 (MQ=255)                         atcatGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATaa                                                                                                              >  1:712984/1‑140 (MQ=255)                             tGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATcc                                                                                                          >  1:218403/1‑140 (MQ=255)                             tGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTACTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGTTCCCCTATCGTCGCATTGGTGATAAATcc                                                                                                          >  1:303210/1‑140 (MQ=255)                                aTCACTTTTCCGTCTTTCTCGTGACCTGTAAGCAGCATAGCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGc                                                                                                       <  2:339442/140‑1 (MQ=255)                                  cACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCAt                                                                                                     >  2:193486/1‑140 (MQ=255)                                            tCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccgccgc                                                                                           >  2:730644/1‑140 (MQ=255)                                             cTTTATCGTCTCCTGTAATCAGTATTTCGTCCTGGTACCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccgccgcc                                                                                          <  1:275337/140‑1 (MQ=255)                                             cTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccgccgcc                                                                                          <  1:193486/140‑1 (MQ=255)                                             cTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccgccgcc                                                                                          <  2:581216/140‑1 (MQ=255)                                             cTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGACATCTgccgccgcc                                                                                          <  1:301676/140‑1 (MQ=255)                                              tttATCGTCACCTGTGATCAGCCTACCGAACTGGTAGACGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCt                                                                                         <  1:417997/140‑1 (MQ=255)                                              tttATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCt                                                                                         <  1:833150/140‑1 (MQ=255)                                                 aTCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccg                                                                                               >  1:88586/1‑131 (MQ=255)                                                 aTCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTgccg                                                                                               <  2:88586/131‑1 (MQ=255)                                                 aTCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCAc                                                                                      >  2:754210/1‑140 (MQ=255)                                                      cACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGa                                                                                 >  1:712662/1‑140 (MQ=255)                                                       aCCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGag                                                                                >  1:855964/1‑140 (MQ=255)                                                        ccTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGagc                                                                               <  2:672715/140‑1 (MQ=255)                                                          tGTAATCAGCATATCCACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGagcag                                                                             <  1:277123/140‑1 (MQ=255)                                                              aTCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCAcc                                                                         <  1:800659/140‑1 (MQ=255)                                                                 aGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAAt                                                                      >  1:108570/1‑140 (MQ=255)                                                                   cATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGGCGCAGCTTCACCGAGAGCAGCACCAATGt                                                                    >  1:361768/1‑140 (MQ=255)                                                                     tatCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGttt                                                                  <  1:730644/140‑1 (MQ=255)                                                                     tatCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGttt                                                                  <  1:428458/140‑1 (MQ=255)                                                                       tCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAg                                                                <  1:187110/140‑1 (MQ=255)                                                                       tCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAg                                                                <  2:25416/140‑1 (MQ=255)                                                                        cGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGc                                                               <  2:712662/140‑1 (MQ=255)                                                                             tGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTcc                                                          >  1:447676/1‑140 (MQ=255)                                                                             tGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTcc                                                          >  2:42755/1‑140 (MQ=255)                                                                               gTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTa                                                        >  2:849027/1‑140 (MQ=255)                                                                                      gCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAgg                                                 <  2:447676/140‑1 (MQ=255)                                                                                         tCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGGGAGCAGCGCCAGTGGTTAGCGTTCCTACAGCAGGCTc                                              >  1:270097/1‑140 (MQ=255)                                                                                              gTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCaaaaa                                         <  1:849027/140‑1 (MQ=255)                                                                                                tATTGGTATGATTAAAGCCAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAaca                                       <  2:540381/140‑1 (MQ=255)                                                                                                tATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAaca                                       <  1:42755/140‑1 (MQ=255)                                                                                                tATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAaca                                       <  2:740203/140‑1 (MQ=255)                                                                                                 aTTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAacac                                      <  2:108570/140‑1 (MQ=255)                                                                                                    ggTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTa                                   >  2:143726/1‑140 (MQ=255)                                                                                                     gTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTaa                                  >  2:69087/1‑140 (MQ=255)                                                                                                       aTGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGt                                >  1:692668/1‑140 (MQ=255)                                                                                                       aTGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGt                                >  2:734483/1‑140 (MQ=255)                                                                                                        tGATTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTa                               <  1:69087/140‑1 (MQ=255)                                                                                                          aTTAAAGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTaaa                             >  1:21567/1‑140 (MQ=255)                                                                                                               aGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACtttt                        >  1:85487/1‑138 (MQ=255)                                                                                                               aGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTtct                        <  2:218403/140‑1 (MQ=255)                                                                                                               aGACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTtct                        >  2:479062/1‑140 (MQ=255)                                                                                                               aGACAAAAACGCCTTAGCCAAGACCCAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTtct                        <  1:266311/140‑1 (MQ=255)                                                                                                                gACAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTtctc                       >  2:786768/1‑140 (MQ=255)                                                                                                                  cAAAAACGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTACCTGCAGGAGGCTCAAAAACATCTAAGTAAACTTCTCCa                     >  2:374778/1‑140 (MQ=255)                                                                                                                    aaaaCGCCTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTGTAGCGTTCGTACTGCAGGCTCAAAAGCACCAAAGTAAACTTCTCCAcc                   >  2:218749/1‑140 (MQ=255)                                                                                                                           cTTCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCGGGCTCAAAAACACCTATGTAAACTTCTCCAACTTCGACa            >  2:401243/1‑140 (MQ=255)                                                                                                                             tCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACAtt          >  2:213366/1‑140 (MQ=255)                                                                                                                             tCGCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACAtt          >  2:739086/1‑140 (MQ=255)                                                                                                                               gCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCa        <  1:143726/140‑1 (MQ=255)                                                                                                                               gCCAAGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCa        <  1:479062/140‑1 (MQ=255)                                                                                                                                  aaGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCg     <  1:213366/140‑1 (MQ=255)                                                                                                                                  aaGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCg     <  2:692668/140‑1 (MQ=255)                                                                                                                                   aGACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGACTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCGt    >  2:515155/1‑140 (MQ=255)                                                                                                                                    gACCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCGTa   >  2:475753/1‑140 (MQ=255)                                                                                                                                     aCCAAGCTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCGTAc  <  2:808067/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACACCTAAGTAAACTTCTCCACCTTCGACATTCAGCGTAC  >  NC_000913/1595954‑1596225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑