Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	205,353	G→A	L76L (CTG→CTA)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	205,353	0	G	A	100.0%	102.5 / NA	31	L76L (CTG→CTA)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

GCGTTGGGTATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGC  >  NC_000913/205222‑205486                                                                                                                                    |                                                                                                                                      gcgTTGGGTATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGtga                                                                                                                               <  2:840347/140‑1 (MQ=255) gcgTTGGGTATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGtga                                                                                                                               <  2:524627/140‑1 (MQ=255) gcgTTGGGTATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGtga                                                                                                                               <  1:112396/140‑1 (MQ=255)         tATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTATACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTaa                                                                                                                       >  2:861292/1‑140 (MQ=255)                  aCTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGa                                                                                                              <  2:43320/140‑1 (MQ=255)                          tCACCGATTTCACCAACCTTTGTGGTCTGGTTAAGGTCTTCGGAGCGGTACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTg                                                                                                      <  2:296473/140‑1 (MQ=255)                             ccGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCAGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTACCCCACCTGACGGTACTggcg                                                                                                   <  2:22223/140‑1 (MQ=255)                                               gtgGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAATCCACCTGGCGGAACTGGCGGCTAACAATACCGGCTAt                                                                                 >  1:254099/1‑140 (MQ=255)                                                     tGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAAt                                                                           >  2:436027/1‑140 (MQ=255)                                                       gTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCC‑CCTGACGGTACTGGCGGCGAACAATACCGGCTAt                                                                                 >  1:526089/1‑131 (MQ=255)                                                       gTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCC‑CCTGACGGTACTGGCGGCGAACAATACCGGCTAt                                                                                 <  2:526089/131‑1 (MQ=255)                                                        tGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTg                                                                        >  1:740172/1‑140 (MQ=255)                                                             ttCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGtt                                                                   >  1:156142/1‑140 (MQ=255)                                                              tCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTg                                                                  <  1:657772/140‑1 (MQ=255)                                                                tACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCt                                                                <  2:792487/140‑1 (MQ=255)                                                                 aCGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCt                                                                >  1:760100/1‑139 (MQ=255)                                                                 aCGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCt                                                                <  2:760100/139‑1 (MQ=255)                                                                 aCGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTg                                                               >  2:209765/1‑140 (MQ=255)                                                                 aCGGAGCAGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACCGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTg                                                               <  1:487634/140‑1 (MQ=255)                                                                         ggACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCCGCTATCAGAATCTGACGTTGCTGATCTCaaa                                                       >  1:863844/1‑140 (MQ=255)                                                                                           aaGCCTATCGTCGGGGCTGATTTTAACGTCCCGTGGGACCTACTGGGTCATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTa                                     <  2:254099/140‑1 (MQ=255)                                                                                                 aTCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGATAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTgc                               >  1:190953/1‑140 (MQ=255)                                                                                                  tcgtcgGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTgcc                              >  2:75768/1‑140 (MQ=255)                                                                                                   cgtcgGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGGCGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTgccc                             >  1:533157/1‑139 (MQ=255)                                                                                                     tcgGGGCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTgccgcc                           <  1:547019/140‑1 (MQ=255)                                                                                                        gggCAGGTTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTCACGTTGCTAATCGCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCggg                        >  2:331976/1‑140 (MQ=255)                                                                                                         ggCAGATTTTAACGTCCAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGGCGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGc                       <  2:533157/140‑1 (MQ=255)                                                                                                                        ccAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAGAAGCGTATGAGCTCGGTTACGGTGCTGCCGGGCCGATCATCGATcgcg        >  2:289376/1‑140 (MQ=255)                                                                                                                        ccAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTAAGGTGCCGCCGGGCCGATCATCGATcgcg        >  2:60340/1‑140 (MQ=255)                                                                                                                         cAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGa       >  1:441020/1‑140 (MQ=255)                                                                                                                         cAGTGCGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGa       >  2:180595/1‑140 (MQ=255)                                                                                                                              cGACCTACTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGc  <  1:209765/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GCGTTGGGTATGCCAGCACTGGCGATCACCGATTTCACCAACCTTTGTGGTCTGGTGAAGTTCTACGGAGCGGGACATGGCGCAGGGATTAAGCCTATCGTCGGGGCAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGC  >  NC_000913/205222‑205486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑