Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,584	A→G	M70V (ATG→GTG)	gstA →	glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,584	0	A	G	100.0%	162.9 / NA	47	M70V (ATG→GTG)	gstA	glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

CGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCC  >  NC_000913/1714457‑1714719                                                                                                                                |                                                                                                                                        cGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATtgtg                                                                                                                                       >  2:777970/1‑130 (MQ=255)  gTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGCTGACGGTAATTTGCTGACGGAAGGCGTAGCCATTGTGCAGTATCTTGc                                                                                                                            >  2:298778/1‑140 (MQ=255)            aTTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAACGAATGTGCAGTACCTTGCCGACAGCGTc                                                                                                                  >  1:379803/1‑140 (MQ=255)                 aTGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGa                                                                                                             >  1:174204/1‑140 (MQ=255)                 aTGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGa                                                                                                             <  2:841790/140‑1 (MQ=255)                 aTGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGa                                                                                                             >  1:450460/1‑140 (MQ=255)                               cGAAAACGGTGACGATTACTTTTCCGTTAACGCTAAGGGCCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTgg                                                                                               <  2:340030/140‑1 (MQ=255)                               cGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTgg                                                                                               <  1:803957/140‑1 (MQ=255)                               cGAAAACGGTGACGAATACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTgg                                                                                               <  1:347254/140‑1 (MQ=255)                                                  tttGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTc                                                                            >  1:488007/1‑140 (MQ=255)                                                   ttGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTcc                                                                           <  2:174204/140‑1 (MQ=255)                                                    tGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGTCAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTccc                                                                          >  1:734683/1‑140 (MQ=255)                                                      ctgTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGc                                                                        >  1:614578/3‑140 (MQ=255)                                                      ccGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGc                                                                        >  1:785755/1‑140 (MQ=255)                                                      ccGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGc                                                                        >  2:26353/1‑140 (MQ=255)                                                         ttAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCtat                                                                     >  1:550013/1‑140 (MQ=255)                                                           aaCCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATaa                                                                   >  2:619415/1‑140 (MQ=255)                                                                tAAGGGGCAGGTGCCAGCATTGCTGTTGGATGACGGTTCTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCa                                                              <  1:298778/140‑1 (MQ=255)                                                                 aaGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCAt                                                             >  2:76603/1‑140 (MQ=255)                                                                  aGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATc                                                            >  2:702925/1‑140 (MQ=255)                                                                   ggggCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCg                                                           <  1:619415/140‑1 (MQ=255)                                                                   ggggCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCg                                                           <  2:1715/140‑1 (MQ=255)                                                                    gggCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGa                                                          <  1:76603/140‑1 (MQ=255)                                                                        aGGTGCCTGCATTGCTGTTGGAAGACGGTACATTGTTGACGGAAGGCGAAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATgg                                                      <  2:371365/140‑1 (MQ=255)                                                                        aGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATgg                                                      >  2:37828/1‑140 (MQ=255)                                                                         ggTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCtata                                                                    <  1:620626/125‑1 (MQ=255)                                                                         ggTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCtata                                                                    >  2:620626/1‑125 (MQ=255)                                                                              cTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAAt                                                <  2:614578/140‑1 (MQ=255)                                                                              cTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAAt                                                <  2:785755/140‑1 (MQ=255)                                                                               tGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATgg                                                      <  1:130359/133‑1 (MQ=255)                                                                               tGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATgg                                                      >  2:130359/1‑133 (MQ=255)                                                                               tGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGACACCGGTAAACAGTATTTCCCGCTAGAAAAGCATCGAATGGCTGAAtt                                               >  2:257176/1‑140 (MQ=255)                                                                                 cATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTAc                                             >  1:638804/1‑140 (MQ=255)                                                                                  aTTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACGGTACTTCACGCTATAAAACCATCGAATGGCTGAATTACa                                            >  2:330095/1‑140 (MQ=255)                                                                                      ctgctgGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGc                                        >  2:55418/1‑140 (MQ=255)                                                                                        gctgGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCa                                      <  2:488007/140‑1 (MQ=255)                                                                                        gctgGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCa                                      <  2:648813/140‑1 (MQ=255)                                                                                             aTGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAg                                 <  1:26353/140‑1 (MQ=255)                                                                                              tGACGGTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCCATAGAACCATCGAATGGCTGACTTACATCGCCACCGAGc                                >  2:333069/1‑140 (MQ=255)                                                                                                   gTACTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCAt                           >  1:711412/1‑140 (MQ=255)                                                                                                     aCTTTGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATaa                         <  1:37828/140‑1 (MQ=255)                                                                                                         tGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCAt                           >  2:625899/1‑134 (MQ=255)                                                                                                         tGCTGACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCAt                           <  1:625899/134‑1 (MQ=255)                                                                                                             gACGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTca                 <  2:711412/140‑1 (MQ=255)                                                                                                              aCGGAAGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTcac                >  2:54352/1‑140 (MQ=255)                                                                                                                   aGGCGTAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACTGATCTGCATAAAGGTTTCACACctc           >  1:352221/1‑140 (MQ=255)                                                                                                                        tAGCGATTGTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGttt      <  1:702925/140‑1 (MQ=255)                                                                                                                            gATTGTGCAGTATCTTGCCGTCAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGcc  <  2:734683/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCC  >  NC_000913/1714457‑1714719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑