Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,197	T→C	G118G (GGT→GGC)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,197	0	T	C	100.0%	138.3 / NA	40	G118G (GGT→GGC)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

CCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCA  >  NC_000913/1738067‑1738318                                                                                                                                   |                                                                                                                          ccGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGCGCTTATCTGTCGGTTAAGGCGCAAAAACGCTACGATGGCATGCTGGTg                                                                                                                  >  2:398551/1‑140 (MQ=255)   gagaTCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGAt                                                                                                                <  2:601139/140‑1 (MQ=255)      aTCATTGAAGCAGTTCAAAAAACTTACTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGAtgtg                                                                                                             <  1:398551/140‑1 (MQ=255)          ttGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTTCACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCt                                                                                                         <  2:603361/140‑1 (MQ=255)          ttGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCt                                                                                                         <  2:692091/140‑1 (MQ=255)                   ttGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTc                                                                                                          >  2:668862/1‑130 (MQ=255)                   ttGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTc                                                                                                          <  1:668862/130‑1 (MQ=255)                                                acCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTa                                                                   >  1:443065/1‑140 (MQ=255)                                                    tGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGc                                                               >  2:540303/1‑140 (MQ=255)                                                     gATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGa                                                                                >  1:741640/1‑122 (MQ=255)                                                     gATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGa                                                                                <  2:741640/122‑1 (MQ=255)                                                      aTTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTAt                                                                  >  1:114408/1‑135 (MQ=255)                                                      aTTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTAt                                                                  <  2:114408/135‑1 (MQ=255)                                                      aTTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCa                                                             <  1:680688/140‑1 (MQ=255)                                                      aTTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCa                                                             <  1:62841/140‑1 (MQ=255)                                                            ggCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTa                                                                   >  2:842075/1‑128 (MQ=255)                                                            ggCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTa                                                                   <  1:842075/128‑1 (MQ=255)                                                            ggCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATccc                                                       >  2:719845/1‑140 (MQ=255)                                                            ggCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATccc                                                       >  2:806842/1‑140 (MQ=255)                                                            ggCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATccc                                                       >  2:699142/1‑140 (MQ=255)                                                                 tGCGTGGAACAATCTTTAGAAACAGCGGGCATATCAGTCCATGATGGCTCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtgg                                                  <  2:404339/140‑1 (MQ=255)                                                                 tGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTTCTGGCGATGCTTGACGAGTATAGCCATATCCCAAtgg                                                  >  1:272978/1‑140 (MQ=255)                                                                 tGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtgg                                                  >  1:713071/1‑140 (MQ=255)                                                                 tGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtgg                                                  <  2:443065/140‑1 (MQ=255)                                                                 tGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtgg                                                  >  2:454878/1‑140 (MQ=255)                                                                 tGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAAtgg                                                  >  2:515431/1‑140 (MQ=255)                                                                             tCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGCAGAGTATCGCCAGATCCGAATGGTGGTCATGGAcc                                      >  2:365486/1‑139 (MQ=255)                                                                                  agaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAGGAGTATCTCCATATCCTAATGATGGTCATGGACTggggg                                 >  2:234477/1‑139 (MQ=255)                                                                                  agaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGt                                 >  2:12091/1‑140 (MQ=255)                                                                                  agaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGt                                 <  2:623562/140‑1 (MQ=255)                                                                                  agaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGt                                 <  1:806842/140‑1 (MQ=255)                                                                                       cAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGACGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGc                            >  1:576062/1‑140 (MQ=255)                                                                                          cGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCaaa                         >  1:138564/1‑140 (MQ=255)                                                                                              cTTATCTGTCGATGACGGCGCATAATCGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCt                     <  1:365486/140‑1 (MQ=255)                                                                                                           gatgGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATg        <  2:566018/140‑1 (MQ=255)                                                                                                           gatgGCGCAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATg        <  1:515431/140‑1 (MQ=255)                                                                                                                cgcAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCg      >  1:809537/1‑137 (MQ=255)                                                                                                                cgcAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCg      <  2:809537/137‑1 (MQ=255)                                                                                                                cgcAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTc   >  2:528031/1‑140 (MQ=255)                                                                                                                cgcAAAAACGCGTCGATGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTc   >  1:707270/1‑140 (MQ=255)                                                                                                                 gcAAAAACGCGTCGACGGCCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCa  <  2:576062/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                          CCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCA  >  NC_000913/1738067‑1738318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑